Engineering an Improved IgG4 Molecule with Reduced Disulphide Bond Heterogeneity and Increased Fab Domain Thermal Stability

Peters, S., et al., 287(29), 24525-24533, J Biological Chemistry, 2012

The authors present an IgG4 molecule with increased Fab thermal stability and reduced product heterogeneity that potentially offers advantages for the production of IgG4 molecules. The Octet system with Protein A biosensors was used to determine expression levels for IgG1 wild-type, IgG4 wild-type and IgG4 mutants.

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