Endoglin Requirement for BMP9 Signaling in Endothelial Cells Reveals New Mechanism of Action for Selective Anti-Endoglin Antibodies

Nolan-Stevaux, O., et al., 7(12):e50920, PloS One, 2012

This study utilized various techniques to analyze the requirement of Endoglin for endothelial SMAD activation. The Octet RED system was used for a series of Endoglin-binding competition assays with the anti-Endoglin antibodies M999, TRC105, SN6 and SN6h. For the binding competition assays, biotinylated recombinant human Endoglin was loaded onto Streptavidin biosensors. The authors found that M999 and TRC105 competed with each other for binding to their epitope on Endoglin, and that both antibodies significantly inhibited BMP9 binding to Endoglin.

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