Efficient Production of Antibodies Against a Mammalian Integral Membrane Protein by Phage Display

Hotzel, I., et al., 24 (9), 679-689, Protein Engineering Design and Selection, 2011

The authors report on a phage display method for selecting antibody fragments specific for mammalian multispan membrane proteins. The model protein used was claudin-1 (CLDN1), an entry co-receptor used by the hepatitis C virus. For kinetic measurements, biotinylated CLDN1 was loaded onto Streptavidin biosensors on the Octet RED platform and incubated with anti-CLDN1 Fab fragments derived from the screen. Affinity maturation experiments utilized the Octet platform in a similar fashion.

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