Dynamic Evolution and Immunoreactivity of Aptamers Binding to Polyclonal Antibodies Against MPT64 Antigen of Mycobacterium Tuberculosis

Qin LH, et al., 33(7):1199-209, Eur J Clin Microbiol Infect Dis, 2014

In this study, the authors systematically monitored the selection process of aptamers, short single-stranded nucleic acids with high affinity to target molecules, in this case specifically against anti-MPT64 antibodies of Mycobacterium tuberculosis as potential substitutes in the serological diagnosis of TB. The binding affinity of candidate aptamers and the dissociation constant of preponderant aptamers were tested on the Octet platform using Streptavidin biosensors.

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