Disassembly of All SNARE Complexes by N-Ethylmaleimide-sensitive Factor (NSF) is Initiated by a Conserved 1:1 Interaction Between α-Soluble NSF Attachment Protein (SNAP) and SNARE Complex

Vivona S, et al., 288(34), 24984-91, J Biol Chem, 2013

This work presents comparative studies of binding constants and disassembly kinetics for four ternary SNARE complexes and one binary (t-SNARE) complex with NSF and α-SNAP. Interaction studies were performed using the Octet RED system. Biotinylated SNARE complex was loaded onto Streptavidin biosensors for binding kinetics experiments.

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