Direct Transactivator-Transcription Factor IID (TFIID) Contacts Drive Yeast Ribosomal Protein Gene Transcription

Layer, J.H., et al., 285(20), 15489-15499, J Biological Chemistry, 2010

The Rap1 binding domains (RBD) of TATA-binding protein associated factors subunits Taf4 and Taf5 were identified and characterized. The Octet system was used to determine the binding affinities Rap1 to wild-type and mutated Taff4 and Taf5. Biotinylated Rap1 was immobilized on Streptavidin biosensors for these measurements. All the mutants of Taf4 and 5 exhibited lower binding affinities to Rap1 compared to WT.

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