Differential Polyubiquitin Recognition by Tandem Ubiquitin Binding Domains of Rabex-5

Shin, D., et al., 423(4), 757-62, Biochem Biophys Res Commun, 2012

Mutations were introduced in the two ubiquitin binding domains (A20_ZF and MIU) of Rabex-5 protein in an effort to characterize Rabex-5 binding affinities and selectivity for polyubiquitin structures. Tetraubiquitin chains were immobized by coupling to Amine Reactive biosensors on the Octet RED system. Kinetic constants for wild-type and mutant Rabex 5 domain binding to polyubiquitin structures were reported.

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