Detection of Deoxynivalenol using Biolayer Interferometry

Maragos, C.M., 27, 157-165, Mycotoxin Research, 2011

Deoxynivalenol (DON) is a toxin produced by Fusarium graminearum and F. culmorum. These fungi can infest wheat, barley, and corn and cause Fusarium Head Blight, a disease of substantial economic significance worldwide. Because of this, and the toxic nature or DON, extensive monitoring is conducted of commodities and foods. As part of efforts to improve detection of the toxin, the author examined the applicability of the Octet RED system. DON spiked in whole wheat flour was successfully measured, suggesting further development of a rapid quantitative Octet assay for DON in wheat may be possible. For these studies, a DON-BSA conjugate was non-covalently immobilized on aminopropylsilane (APS) biosensors.

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