Crystal Structure and Biochemical Analysis of the Heptameric Lsm1-7 Complex

Zhou L, et al., 24(4):497-500, Cell Res, 2014

The authors present binding affinity results of the Lsm1-7, a heptameric protein complex that functions in mRNA degradation pathway regulation. A crystallized structure of the S. cerevisiae Lsm1-7 complex components were determined and using the Octet system the differential binding affinity and specificity to oligo repeat sequences were analyzed. This work summarizes the LSM1-7 RNA-binding properties as demonstrated by mutational analysis and provides a better understanding of the mechanistic regulation in RNA metabolism.

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