Cross-neutralization of Influenza A Viruses Mediated by a Single Antibody Loop

D.C. Ekiert, et al. , 489(7417), 526-32, Nature, 2012

An antibody (C05) that neutralized strains from multiple subtypes of influenza A virus was isolated by phage display . Functional and structural characterization showed that C05 neutralized multiple subtypes by inserting a single extended CDR loop into the receptor binding pocket of the HA1 subunit. The Octet RED system and ITC were used to measure KDs for C05 Fab binding do a panel of influenza HA subtypes. Biotinylated HAs and Streptavidin biosensors were used for the binding studies.

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