Construction of pH-sensitive Her2-binding IgG1-Fc by Directed Evolution

Traxlmayr MW, et al., 9(8):1013-22, Biotechnol J, 2014

The authors utilized an evolutionary design strategy and a yeast display library to screen for crystallizable fragments of immunoglobulin G1 (Fcab) that were engineered for pH-dependent antigen binding as a means of generating more effective therapeutic proteins that possess longer half-lives. Binding studies were performed on the Octet QK system using Streptavidin and Protein A biosensors.

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