Construction of an Antimyoglobin Single-Chain Variable Fragment with Rapid Reaction Kinetics

Jang J, et al., DOI: 10.1002/bab.1349, Biotechnol Appl Biochem, 2015

This article describes the development of an IgG-type monoclonal anti-human myoglobin antibody using the hybridoma technology. The cloned gene for a single-chain variable fragment (scFv) was originated from IgG-Myo2-7Ds. The gene product is fused to Protein-A (ProA) tag at the C-terminus and expressed in E. coli. The purified scFv was characterized for binding with immobilized Myo using a Pall ForteBio Octet RED system equipped with aminopropyl silane (APS) biosensor probes.

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