Comparable Antigenicity and Immunogenicity of Oligomeric Forms of a Novel, Acute HIV-1 Subtype C gp145 Envelope for Use in Preclinical and Clinical Vaccine Research

Wieczorek L, et al., 89(15):7478-93, J Virol, 2015

The production of a subtype C HIV-1 envelope immunogen from mammalian cells is described. It also demonstrates assembly of gp145 protein into trimeric forms without any engineered trimerization motifs. The gp145 binding to several broadly neutralizing MAbs were measured using a Pall ForteBio Octet RED system equipped with Streptavidin biosensors. Biotinylated gp145 (unfractionated, dimer, trimer) and gp120 were captured onto Streptavidin biosensors and dipped into dilution series of MAbs and sCD4.

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