Cleavage of a Putative Metal Permease in Chlamydia Trachomatis Yields an Iron-dependent Transcriptional Repressor

Thompson, C., et al., 109 (26), 10546-10551, Proc Natl Acad Sci USA, 2012

The authors demonstrate that the C-terminal domain of the CT069 protein in Chlamydia trachomatatis serves as an iron-dependent DNA-binding repressor protein. The Octet QKe system was used to quantitatively characterize binding of the repressor domain of the CT069 protein to a DNA operator sequence. The biotinylated DNA fragment was immobilized using Streptavidin biosensors, and then incubated in solutions containing the repressor domain supplemented with various divalent metals. Only the addition of Fe2+ led to DNA binding. The Octet system then was used to characterize iron-dependent DNA-protein binding kinetics and the dissociation constant.

Read More