Characterization and Epitope Mapping of the Polyclonal Antibody Repertoire Elicited by Ricin Holotoxin-based Vaccination

Cohen O, et al., 21(11):1534-40, Clin Vaccine Immunol, 2014

This is an investigation to obtain information and a plausible strategy for the development of an improved holotoxin-based vaccine against ricin. Ricin is one of the most lethal toxins known to date and yet, there is no cure against ricin exposure despite less effective therapies based on neutralizing antibodies elicited by active vaccination or those given passively are available. An epitope mapping analysis (using phage display random peptide libraries) performed on anti-ricin antibodies revealed immunodominant epitopes in both subunits A and B of ricin. The study also helped to determine the roles of these epitopes in ricin neutralization. An Octet RED instrument equipped with SA biosensors was used to capture biotinylated ricin. These titrations were carried out using serial dilutions of ricin-purified fractions of antibodies.

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