Cellular Uptake and Ultrastructural Localization Underlie the Pro-Apoptotic Activity of a Hydrocarbon-Stapled BIM BH3 Peptide

Edwards A, et al., DOI: 10.1021/acschembio.5b00214, ACS Chem Biol, 2015

This article demonstrates a stepwise approach to the development of clinically relevant stapled peptides by comparing biochemical, biophysical, cellular, and mechanistic investigations. Binding measurements were made using a Pall ForteBio Octet RED384 system equipped with Super Streptavidin (SSA) biosensors. The biotinylated peptides captured onto SSA biosensors were dipped into a serial dilution of BCL-XL ΔC protein.

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