Calcium-independent Inhibition of PCSK9 by Affinity-improved Variants of the LDL Receptor EGF(A) Domain

Zhang Y, et al., 422(5), 685-96, J Molecular Biology, 2012

To generate more potent EGF(A) domain inhibitors of PCSK9, the authors constructed and screened a phage display library. Several EGF variants with improved binding affinities were identified, with one clone displaying significantly increased inhibition and prevention of LDLR degredation. The Octet system was used to determine binding affinities of EGF-Fc fusion proteins to PCSK9.

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