BMI1-RING1B is an Autoinhibited RING E3 Ubiquitin Ligase

Taherbhoy A, Huang O, Cochran A, 5.542361111, Nat Commun, 2015

The authors investigated enzymatic properties of all six RING-RING heterodimers (RING1B-PCGF1 through RING1B-PCGF6) involved in the ubiquitanation process. Affinity measurements carried out using a Pall ForteBio Octet RED384 system equipped with anti-GST biosensors helped reveal subtle differences. Binding curves were generated by capturing GST-RING1B-PCGF constructs and titrating against UbcH5c(S22R/C85K) or UbcH5c(S22R/C85K)~Ub. A steady state analysis of the binding curves was used to obtain KD values.

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