Biophysical Measurement of the Balance of Influenza A Hemagglutinin and Neuraminidase Activities

Benton D, et al., 290(10):6516-21, J Biol Chem, 2015

The application of Bio-Layer Interferometry to study the interdependence of hemagglutinin (HA) and neuraminidase (NA) activities pertaining to Influenza A virus (IAV) is reported. In order to mitigate pandemic strains, it is important to have a better understanding of characteristics such as the HA/NA balance. Particularly the binding and release of virus from receptor analogs immobilized onto a biosensor surface were investigated. An Octet RED system equipped with Streptavidin biosensors was used to capture biotinylated receptor analogs (sugars). The binding of virus monitored in real time in the presence and absence of NA inhibitors allowed understanding of the mechanistic aspects of virus transmissibility.

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