Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Shah NB and Duncan TM, (84):e51383, J Vis Exp, 2014

This video highlights the use of BLI to study inhibitory interactions of the subunit e with the catalytic complex of E. coli ATP synthase as a means to better generate new antibacterial drugs. Binding studies were performed on the Octet RED96 system and Streptavidin biosensors were used.

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