Binding Rate Screen - A High-Throughput Assay in Soluble Lysate for Prioritizing Protein Expression Constructs

Tian-Yu, J., et al., 399(2), 276-283, Analytical Biochemistry, 2010

This publications reports on the Binding Rate Screen, a functional screen for prioritizing expression constructs in crude soluble lysates. In this approach, the association rate of hexahistinine tags provides information on both the tagged protein concentration and the tag accessibility (a probe of protein folding and/or aggregation). A biotinylated penta-His antibody was used with Streptavidin biosensors for binding measurements on the Octet system.

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