Bactericidal Monoclonal Antibodies Specific to the Lipopolysaccharide O Antigen from Multidrug-Resistant Escherichia coli Clone ST131-O25b: H4 Elicit Protection in Mice

Szijarto V, et al, 59:3109-3116, Antimicrob Agents Chemother, 2015

Described herein is the generation and selection of a humanized Monoclonal Antibody (MAb) capable of binding to a conserved antigen called lipopolysaccharide O25b. In particular, the development of an antibody therapeutic against multidrug-resistant bacteria (gram-negative) species is discussed. Bio-Layer Interferometry (BLI) measured the binding of candidate antibodies. The polysaccharide antigen O25b was biotinylated and captured onto Streptavidin biosensors. The monitoring of association and dissociation of MAbs at varying concentrations allowed determination of the KD. Additionally, the BLI evaluation of polyreactivity (against commercially available unrelated antigens) was achieved by immobilizing the MAbs via Anti-human Capture Biosensors.

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