Aptamers as a Sensitive Tool to Detect Subtle Modifications in Therapeutic Proteins

R Zichel, et al., 7(2), e31948, PLoS ONE, 2012

This study demonstrate proof-of-principle that DNA aptamers to thromin can be used as surrogate antibodies to characterize thrombin conformational changes. Thrombin binding afinities and kinetic rate constants for six anti-human thrombin aptamers were determined using the Octet RED96 system. Biotinylated aptamers were immobilized on Streptavidin biosensors for these studies. Following heat treatment of recombinant thromin, changes in dissociation rate were reported for 4 of the 6 aptamers.

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