Aptamers as a Replacement for Antibodies in Enzyme-Linked Immunosorbent Assay

Toh s, et al., 64:392-403, Biosens Bioelectron, 2015

This is a review article describing the utility of aptamers in place of antibodies in Enzyme-Linked Apta-Sorbant Assay (ELASA). Authors are drawing parallels between ELISA and ELASA in terms of their capability to identify target molecules such as antigens on cellular surfaces, allergens in environmental samples, and contaminants in food. A special emphasis is given to Bio-Layer Interferometry (BLI) based ELASA due to its applicability to aptamer based diagnostic assay development.

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