Application of Bio-Layer Interferometry for the Analysis of Protein/Liposome Interactions

Wallner, J, et al., 72, 150-4, J Pharm Biomed Anal, 2013

A method to characterize protein/liposome binding interactions is described. Binding of three liposome formulations (DDPC, egg-PC and POPC) to recombinant human erythropoietin (rh-Epo) on the Octet QK platform was the model system. Binding experiments used Streptavidin biosensors loaded with biotinylated rh-Epo, incubated with various liposomes at different concentrations to measure the corresponding association and dissociation profiles.

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