Antigenicity and Immunogenicity of a Trimeric Envelope Protein from an Indian Clade C HIV-1 Isolate

Sneha Priya R, et al., 290(14):9195-208, J Biol Chem, 2015

This article characterizes gp145 from a CCR5 Indian clade C HIV-1 (93IN101) isolated from 293 cells. Data suggest the ability of gp145 to induce broadly neutralizing antibodies by exposing conserved regions of the protein. The findings also suggest the potential of trimeric gp145 to be used as a multiclade candidate HIV-1 vaccine. The characterization of binding affinities between 93IN101 glycoproteins with CD4 or various monoclonal antibodies was achieved by a Pall ForteBio Octet RED system. The glycoproteins (gp120 and gp145) were biotinylated and captured onto Streptavidin Biosensor probes. These binding assays involved screening against varying concentrations of IgG.

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