Allosteric Peptide Activators of Pro-Hepatocyte Growth Factor Stimulate Met Signaling

Landgraf, K.E., et al., 285, 40362-40372, J Biological Chemistry, 2010

Hepatocyte growth factor (HGF) activates Met receptor tyrosine kinase in a therapeutically important signaling sequence. The authors performed Met binding assays on the Octet RED system to show that an 8 amino acid peptide allosterically enhances pro-HGF beta binding to Met protein with an apparent affinity constant of 1.6 micromolar. For these experiments, biotinylated Met ECD was immobilized on Streptavidin biosensors.

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