Acetylproteomic Analysis Reveals Functional Implications of Lysine Acetylation in Human Spermatozoa (sperm)

Yu H, et al., 14(4):1009-23, Mol Cell Proteomics, 2015

This investigation has focused on identifying the global lysine acetylation profile (acetyl proteome) of human sperm with the help of a pan-anti-acetyllysine monoclonal antibody. The authors discuss several factors correlating lysine acetylation and sperm functions. An Octet RED96 system equipped with Streptavidin Biosensor probes was utilized to characterize the binding between mAb and BSA conjugated acetyllysine peptide. After synthesis, BSA-P1 and BSA-P1(Ac) biotinylated and captured onto Streptavidin Biosensor probes. A serial dilution of antibody 1G5 was used as the analyte.

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