A Non-Active-Site SET Domain Surface Crucial for the Interaction of MLL1 and the RbBP5/Ash2L Heterodimer within MLL Family Core Complexes

Shinsky SA, et al., pii: S0022-2836(14)00156-9, J Mol Biol, 2014

The authors performed mutagenic analysis on mixed lineage leukemia-1 (MLL1) enzyme, a histone H3 lysine 4 monomethyltransferase which has been implicated in many human developmental disorders and cancers in order to better understand its mechanism of action and involvement with the SET1 family core complexes. Binding studies were performed on the Octet RED system and Streptavidin biosensors were used.

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