A Human Monoclonal IgG That Binds Aβ Assemblies and Diverse Amyloids Exhibits Anti-Amyloid Activities In Vitro and In Vivo

Levites Y, et al., 22;35(16):6265-76, J Neurosci, 2015

This article reports isolation of an affinity matured human monoclonal antibody with the ability to recognize pan-amyloid epitope, and with a demonstrated specificity for aggregated amyloid forms. The authors used an affinity matured IgG known as 3H3 in this study. Several techniques including the ELISA, immunohistochemistry, and competitive binding assays employed in this investigation. An Octet RED 384 system equipped with Streptavidin biosensors was used to carry out affinity determinations and off-rate screening assays. The monomeric forms of Aβ40 or Aβ42 were biotinylated and captured via Streptavidin biosensors. Binding experiments involved various forms of 3H3 or pan-Aβ antibody dilutions prepared in a 384-well plate.

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