A High Cell Density Transient Transfection System for Therapeutic Protein Expression Based on a CHO GS-Knockout Cell Line: Process Development and Product Quality Assessment

Rajendra Y, et al., 112(5):977-86, Biotechnol Bioeng, 2015

This article describes the optimization of transfection and protein expression in CHO K1SV GS KO cells by using a variety of strategies such as the optimization of DNA, polyethyleneimine (PEI), cell density as well as several other process-development strategies. The protein concentration estimations in culture media were carried out using an Octet QK system.

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