14-3-3 Proteins Interact with a Hybrid Prenyl-Phosphorylation Motif to Inhibit G Proteins

Riou, P, et al., 153(3), 640-53, Cell, 2013

This study identifies 14-3-3 proteins as Rnd interaction partners, and further shows that 14-3-3 proteins can act as solubilizing factors for phosphorylated and prenylated proteins from the Ras superfamily. The authors use the Octet RED96 system to characterize binding between 14-3-3 proteins and various phosphorylated, isoprenylated, geranylated, farnesylated and geranylgeranylated peptides. Biotinylated peptides were loaded onto Streptavidin biosensors and interacted with 14-3-3 proteins to generate kinetic and affinity constants.

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