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Eight filter options provide simplified assay setup

The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to each reading.

  • Cover a wide range of applications

    Cover a wide range of applications

    Eight filters cover a wide range of applications—ELISAs and immunoassays; protein quantitation such as Bradford, Lowry, BCA, and DC protein assays; phosphatases and kinases; cell viability.

  • Simplify assay setup

    Simplify assay setup

    SoftMax® Pro Software simplifies assay setup with predefined protocols, standard data reduction settings, automatic data recovery, and result visualization and analysis.

  • Customize options

    Customize options

    Customize a variety of options such as discontinuous kinetics feature for pausing and resuming kinetic reads, predefined calculation options for common data analysis functions, and easy data export.

ELISA Workflow using the EMax plus Microplate Reader

ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer

Features

  • Easy data visualization

    Powerful curve fitting protocols and statistical analysis features are included for easy visualization of acquired data as grayscale or color map images, 3D graphs, kinetic plots, or reaction rates.

  • Compact design

    The small footprint and low profile saves space on the bench.

Applications of EMax Plus Microplate Reader

  • Absorbance

    Absorbance

    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

    Learn more 

    ELISA

    Elisa

    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

    Learn more 

  • Protein Quantitation (Bradford, Lowry, BCA, DC)

    Protein Quantitation

    Protein concentration can be measured directly, via absorbance at 280 nm in a UV spectrophotometer, or indirectly, using colorimetric methods such as BCA or Bradford assays. Absorbance quantitation is easy to do as it does not require additional reagents, but colorimetric methods offer greater sensitivity and are often preferred when samples are precious. Both can be performed in a UV-vis spectrophotometer or in an absorbance microplate reader.

    Read Application Note 

Specifications & Options of EMax Plus Microplate Reader

 

*Using lowest settings and speed read when available.

Resources of EMax Plus Microplate Reader

Presentations
Videos & Webinars
ELISA Workflow using the EMax plus Microplate Reader

ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer

  • Citation
    Dated: Dec 14, 2020
    Publication Name: Brain, Behavior, and Immunity

    Cytokine Dysregulation Associated with Exam Stress in Healthy Medical Students

    The mechanisms of stress-related immune alterations have not been fully elucidated. Cell-mediated immune responses as well as antibody and certain cytokines are reported as being suppressed during times of high stress. However, the role of suppression vs dysregulation has not been established in human stress models. The effect of exam stress on… View more

    The mechanisms of stress-related immune alterations have not been fully elucidated. Cell-mediated immune responses as well as antibody and certain cytokines are reported as being suppressed during times of high stress. However, the role of suppression vs dysregulation has not been established in human stress models. The effect of exam stress on regulatory cytokines in 16 healthy medical students was assessed by measuring type-1 (IFN-γ) and type-2 (IL-10) cytokines from 72-h PHA/PMA-stimulated PBMC 4 weeks before and 48 h after exams. Results demonstrated decreased IFN-γ accompanied by increased IL-10 during exam stress that resulted in a decreased IFN-γ:IL-10 ratio. There was a significant correlation between the cytokine response to PHA/PMA and number and subjective adjustment to daily hassles. Additionally, students who reported greater levels of loneliness also reported greater numbers of and poorer subjective adjustment to hassles. The differences were consistent in both males and females but did not correlate with AM cortisol levels. Additionally, when individuals were grouped into high vs low preexam hassle levels, the type-1/type-2 shift in the IFN-γ:IL-10 ratio occurred in the low hassles group only. These data suggest that psychologically stressful situations shift type-1/type-2 cytokine balance toward type-2 and result in an immune dysregulation rather than overall immunosuppression. This may partially explain the increased incidence of type-2-mediated conditions such as increased viral infections, latent viral expression, allergic/asthmatic reactions, and autoimmunity reported during periods of high stress.

    Contributors: Gailen D.Marshall Jr, Sandeep K. Agarwal, Camille Lloyd, Lorenzo Cohen, Evelyn M. Henninger, Gloria J. Morris  
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  • Citation
    Dated: Jun 07, 2008
    Publication Name: J. Microbiol. Biotechnol

    Investigation of the Antifungal Activity and Mechanism of Action of LMWS-Chitosan

    Chitosan, a cationic polysaccharide, has been widely used as a dietary supplement and in a variety of pharmacological and biomedical applications. The antifungal activity and mechanism of action of low molecular weight water-soluble chitosan (LMWS-chitosan) were studied in fungal cells and vesicles containing various compositions of fungal lipids… View more

    Chitosan, a cationic polysaccharide, has been widely used as a dietary supplement and in a variety of pharmacological and biomedical applications. The antifungal activity and mechanism of action of low molecular weight water-soluble chitosan (LMWS-chitosan) were studied in fungal cells and vesicles containing various compositions of fungal lipids. LMWS-chitosan showed strong antifungal activity against various pathogenic yeasts and hyphae-forming fungi but no hemolytic activity or cytotoxicity against mammalian cells. The degree of calcein leakage was assessed on the basis of lipid composition (PC/CH; 10:1, w/w). Our result showing that LMWS-chitosan interacts with liposomes demonstrated that chitosan induces leakage from zwitterionic lipid vesicles. Confocal microscopy revealed that LMWSchitosan was located in the plasma membrane. Finally, scanning electron microscopy revealed that LMWS-chitosan causes significant morphological changes on fungal surfaces. Its potent antibiotic activity suggests that LMWS-chitosan is an excellent candidate as a lead compound for the development of novel anti-infective agents

    Contributors: Park, Yoonkyung, Mi-Hyun Kim, Seong-Cheol Park, Hyeonsook Cheong, Mi-Kyeong Jang, Jae-Woon Nah, and Kyung-Soo Hahm  
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  • Citation
    Dated: Oct 01, 1995
    Publication Name: The Journal of Infectious Diseases

    Drug Cytotoxicity Assay for African Trypanosomes and Leishmania Species

    The trypanosomes and Leishmania species are parasitic protozoa that afflict millions of people throughout the world. If not treated, African trypanosomiasis and visceral leishmaniasis are fatal. The available drugs are severely limited by toxicity, marginal efficacy, the requirement for parenteral administration, and spreading drug resistance. In… View more

    The trypanosomes and Leishmania species are parasitic protozoa that afflict millions of people throughout the world. If not treated, African trypanosomiasis and visceral leishmaniasis are fatal. The available drugs are severely limited by toxicity, marginal efficacy, the requirement for parenteral administration, and spreading drug resistance. In this study, a spectrophotometric assay was developed and validated for measuring the cytotoxicity of test compounds against axenically cultured bloodstream-form Trypanosoma brucei (African trypanosomes) and promastigotes of Leishmania donovani. Enzymatic hydrolysis of p-nitrophenyl phosphate, monitored by a microtiter plate reader, is a reliable surrogate for parasite cell counts. The assay is simple, inexpensive, and highly reproducible. The coefficient of variation for EC50 values is <10% for determinations obtained over several months. This method permits the rapid screening of candidates for much-needed new drugs against these parasites.

    Contributors: Annette L. Bodley, Michael W. McGarry, Theresa A. Shapiro  
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