Next generation OneStep® SPR injections enables users to identify hits faster, obtain more information-rich data, and eliminate sample dilutions

Pioneer SPR systems provide high quality kinetic data (ka, kd) and affinity measurements (KD) to chracterize a wide range of biomolecular interactions from small molecule fragments to biologics. OneStep SPR injections enable faster assay workflows where a single analyte concetration is sufficient for accurate kinetics and affinity determination.   

  • Simplify kinetic chracterizations

    Simplified kinetic chracterizations

    With OneStep injections, users can obtain accurate kinetic and affinity values from one analyte concentration eliminating the need to run multiple analyte titrations saving time and error associated with making dilutions. NextStep injections provide a simple workflow to characterize competitive binders against a ligand to capture the mechanism of action.

  • Robust fluidics design

    Robust fluidics design

    Pioneer fluidic system is made up of inert materials such as PEEK, ceramic and Tefzal providing durability and low maintenance.

  • Low baseline noise

    Low baseline noise, minimal drift, high sensitivity and up to 40 Hz data collection rate

    Extremely low baseline noise and drift enable measurement of reliable kinetics and affinity values for high affinity and challenging binders. With up to 40 Hz of data acquisition capabilities, Pioneer FE systems are capable of capturing very fast kinetic association and dissociation phases of a wide range of biomolecules and small molecule fragments.

One Step SPR

One Step SPR

Features

  •  fully automated SPR system

    3 channel, fully automated SPR system

    With three sensing channels, two target proteins or ligands can be analyzed simultaneously for binding with one surface utilized as a reference channel.

  • Screen 768 fragments in 24 hours

    Screen 768 fragments in 24 hours

    Pioneer FE platform's high-throughput screening feature enables kinetic screening of 768 small molecule fragments in a single experiment in 24 hours.

  • unattended run time

    Up to 72 hours of unattended run time

    Offers a variety of sample vessel formats with up to two 384 well plate capacity with an unattended runtime of about 72 hrs for performing large binding screens.

  • Rapid data analysis with QDat

    Rapid data analysis with QDat software and hit selection feature

    Pioneer SPR systems are equipped with an integrated, industry-proven data analysis software based on Scrubber and Clamp platforms. Raw data across multiple runs, days, and sensors can be combined and analyzed using binding algorithms such as single site, multi-site binding, mass transport, and irreversible inhibitor analysis as required for analysis. Hit-selection software feature enables rapid identificatoin of frgment hits that are acquired by Pioneer FE SPR systems.

  • Gain more data with less

    Gain more data with less

    As the analyte gradient is generated in OneStep injections, the diffusion coefficient is calculated that provides an assessment of aggregation enabling users to gain information not only on kinetics and affinity but also on aggregation in just one injection. 

Applications for Pioneer and Pioneer FE Systems

  • Characterization of high affinity biologic interactions

    Characterization of high affinity biologic interactions

    Target binding characterization is an essential analytical step for the selection of high affinity (KD <1 nM) and highly specific biologics regardless of the types of molecules. A kinetic analysis further describes the components of association and dissociation that comprise the overall affinity interaction. For example, two lead compounds may possess a similar affinity (KD) to its target, but their differences in kinetic rate constants of association and dissociation can be used to estimate which will be more useful in vivo. Accurate analysis of these kinetic rate constants is therefore important information for lead selection and predicting the efficacy of protein therapeutics. The Pioneer SPR system with next generation SPR injections improves the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. The next-generation OneStep® gradient injection featured on the Pioneer platform dramatically increases the speed of affinity characterization while maintaining accuracy and high confidence in results.

    Read Application Note  

  • Characterize Irreversible Inhibitors

    Characterize irreversible inhibitors and measure commitment to covalency

    The majority of small molecule inhibitor assays tested with label-free, real-time biosensor technologies are reversible interactions, characterized by commonly used kinetic rate models. However, a significant fraction of therapeutic enzyme inhibitors on the market functions through covalent modification of the target. ForteBio's Pioneer FE is a SPR platform that can be used with regenerable Streptavidin biosensors to reversibly capture protein targets and quantify the efficiency of covalent inhibitors binding to the target. The Pioneer FE system's irreversible inhibitor applications method can be used to determine inhibitor compound's commitment to covalency (Cc) as a metric for irreversible inhibitors.

    Read Application Note  

  • Fragment screening

    Fragment screening

    Fragment-based drug design has become an increasingly popular platform for the identification of lead candidates in drug discovery programs. The detection and characterization of fragment binding events is facilitated by sensitive biophysical technologies capable of detecting low affinity interactions of low molecular weight compounds. Surface plasmon resonance (SPR) has become one of the core technologies used in the identification of these low-affinity fragment compounds. SPR-based biosensors such as the Pioneer FE have the necessary sensitivity and throughput to provide complete fragment screens on libraries of several thousand compounds in just a few weeks per target.

    Read Application Note  

Specification & Options of Pioneer and Pioneer FE Systems

Resouces of Pioneer and Pioneer FE Systems

Ordering information of Pioneer and Pioneer FE Systems

Product Part
Number
Pioneer Biosensors – CDL 19-0127
Pioneer Biosensors – CDH 19-0128
Pioneer Biosensors – PCH 19-0129
Pioneer Biosensors – SADH 19-0130
Pioneer Biosensors - HisCap PS05AFB
Pioneer Biosensors - COOH1 PS00AFB
Accessories and Adapters
Plate Adaptor Greiner 384DW, 225 µL, each PM113FB
Plate Adaptor Greiner 384wp, 120 µL, each PM120FB
Plate Adaptor, Axygen 384DW, 240 µL, each PM018FB
Plate Adaptor, Axygen P96450 450 µL, each PM015FB
Plate Adaptor, Axygen PCR96, each PM046FB
Plate Adaptor, Axygen PDW11 1 mL, each PM016FB
Plate Adaptor, Greiner 96ubot Plate 280 µL PM114FB
Plate Adaptor, Greiner 96ubtCW Plate 300 µL PM115FB
Consumables
Cartridge Buffer Filter Replace, 10 µm PM107FB
Clean Station Cover, each PM081FB
Microbeaker, 7.5 mL PM004FB
Seal, 384-Well Precut, Pack of 5 PM009FB
Seal, 8 mm 96 Well Rack Precut, Pack of 5 PM008FB
Seal, Front Vial Holder, Pack of 5 PM104FB
Seal, Pierce Teflon 384SqW Plate, Pack of 20 PM134FB
Seal, Pierce Teflon 96wp Plate, Pack of 20 PM135FB
Seal, Rear Vial Holder, Pack of 5 PM103FB
Seal, Varied Sample Rack, Precut, Pack of 5 PM010FB
Seals, PCR96 Microplates Precut, Pack of 5 PM007FB
Vials, 12 mm, Box of 100 PM005FB
Vials, 8 mm, Set of 200 PM006FB

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.