Personal assay BLItz System
Label-free assays in a drop
Drop. Read. Done.
The BLItz® System brings label-free analysis to individual researchers, and is affordably priced so everyone can have one. With this personal assay system, you can run protein quantitation and kinetics experiments in only 4 µL of sample—right at your own lab bench.
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Simple and rapid
The BLItz System enables the detection of specific mAbs, even in unpurified samples, in as little as 30 seconds.
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Save sample
Affinity, concentration, and binding kinetics can be measured right at the bench in a 4 µL drop of sample.
Label-Free Analysis on BLItz System
Features
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Instant protein presence
Detect the presence or absence of active proteins in just a drop of sample, and easily identify specific proteins in complex solutions. Data from the BLItz System complements existing methods like blots and gels, and its real-time binding curves give instant visual confirmation.
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Faster protein quantitation
Measure protein concentration without labeling or secondary reagents — even in crude media. With a wide dynamic range and sensitivity down to a few ng/mL, the BLItz System gives you accurate quantitation in seconds compared to traditional methods that can often take hours.
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Easy reagent QC testing
Rapidly identifying specific proteins with immunoassay-based techniques like ELISA or HPLC add many hours to the process and delay results. With the BLItz System, proteins and antibodies can be quantitated in a matter of seconds with high specificity and sensitivity, even in crude samples.
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Effortless kinetics
Get rate and affinity constants for binding interactions (ka, kd, KD) in minutes using only 4µL of sample. With both a small price and a small size, the BLItz System can be your own personal label-free kinetics system. Ready to go right out of the box, its simple workflows let you run assays right at your lab bench with ease.
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Smarter assay development
Monitoring the effects of changing conditions on binding interactions in real time, the BLItz System lets you develop better immunoassays in only a few minutes. Find the best antibody pairs, identify the best assay parameters, and even build and optimize an ELISA step by step.
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Application Note
Best practices for performing quantitation assays using the BLItz System
Best practices for performing quantitation assays using the BLItz System
The BLItz® system provides a simple, rapid Dip and Read™ approach for protein quantitation in a small, affordably priced personal assay system. With BLItz, proteins and antibodies can be…
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Application Note
Instant Determination of Protein Presence Using the BLItz System
Instant Determination of Protein Presence Using the BLItz System
The BLItz system provides a simple, rapid Dip and Read™ approach to protein analysis in an affordably priced personal assay system. Protein and antibody detection can be performed in a…
Applications for personal assay system – BLItz System
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Easily Determine Interactions Kinetics
Get rate and affinity constants for binding interactions (ka, kd, KD) in minutes using only 4 µL of sample. With both a small price and a small size, the BLItz system can be your own personal label-free kinetics system. Ready to go right out of the box, its simple workflows let you run assays right at your lab bench with ease.
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Rapidly Quantify your Protein with Confidence
Measure protein concentration without labeling or secondary reagents — even in crude media. With a wide dynamic range and sensitivity down to a few ng/mL, the BLItz system gives you accurate quantitation in seconds compared to traditional methods that can often take hours.
-
Instantly Detect Presence of Specific Protein
Detect the presence or absence of active proteins in just a drop of sample, and easily identify specific proteins in complex solutions. Data from the BLItz system complements existing methods like blots and gels, and its real-time binding curves give instant visual confirmation.
Resources for personal assay system – BLItz System
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Application Note
Best practices for performing quantitation assays using the BLItz System
Best practices for performing quantitation assays using the BLItz System
The BLItz® system provides a simple, rapid Dip and Read™ approach for protein quantitation in a small, affordably priced personal assay system. With BLItz, proteins and antibodies can…
-
Application Note
Instant Determination of Protein Presence Using the BLItz System
Instant Determination of Protein Presence Using the BLItz System
The BLItz system provides a simple, rapid Dip and Read™ approach to protein analysis in an affordably priced personal assay system. Protein and antibody detection can be performed in a…
-
Application Note
Fast Quantitation of Proteins and Antibodies Using the BLItz System
Fast Quantitation of Proteins and Antibodies Using the BLItz System
Traditional techniques for determining concentration of a target protein such as ELISA and HPLC are both elaborate and timeconsuming, especially when analyzing complex matrices. For…
Data Sheet
Qualification and Compliance Tools for the BLItz® System
Qualification and Compliance Tools for the BLItz® System
Affordably priced and easy to use, the BLItz system is enabling labelfree detection and analysis across protein manufacturing, purification, and processing workflows. To support require…
Newsletter
NEW Dip and Read™ Anti-Mouse IgG (Fc) Capture (AMC) Biosensors
NEW Dip and Read™ Anti-Mouse IgG (Fc) Capture (AMC) Biosensors
Monoclonal antibodies are key tools for basic research as well as for diagnosis and treatment of human diseases. Therapeutic monoclonal antibodies approached $50 billion in worldwide sa…
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Newsletter
NEW Protein G Biosensor Available for the Octet Platform!
NEW Protein G Biosensor Available for the Octet Platform!
Immunoglobulin-binding proteins such as Protein A, Protein G and Protein L are commonly used to purify, immobilize or detect immunoglobulins. They have different binding profiles with r…
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Newsletter
NEW Ni-NTA Biosensors for HIS-tagged Biomolecule Analysis
NEW Ni-NTA Biosensors for HIS-tagged Biomolecule Analysis
The robustness of the HIS-tag and its ease of use have established it as one of the most commonly used tags in the biopharmaceutical industry. It’s commonly fused to recombinant protein…
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Newsletter
New Protein L Biosensor For Antibody Fragment Quantitation
New Protein L Biosensor For Antibody Fragment Quantitation
Antibodies are so rich in biochemical features that nearly every piece and parcel of their structure has been harvested and put to use, leading to widespread adoption of whole molecules…
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Newsletter
Anti-human IgG (Fc) Capture Biosensors for Immobilization and Kinetic Characterization of Human IgG and Other Fc-containing Proteins
Anti-human IgG (Fc) Capture Biosensors for Immobilization and Kinetic Characterization of Human IgG and Other Fc-containing Proteins
The Anti-Human IgG Fc Capture (AHC) biosensor is designed for kinetic characterization of macromolecular interactions between proteins containing an Fc region and a target analyte. This…
Brochure
BLItz Label-free Assay in a Drop
BLItz Label-free Assay in a Drop
The BLItz® system finally makes label- free analysis available to individual researchers. Priced so everyone can have one, you can now monitor protein binding interactions, quantify pro…
Scientific Poster
Label-free Analysis from Microliter Amounts of Sample
Label-free Analysis from Microliter Amounts of Sample
BLItz instrument – A need was identified for an easy to use, compact, bench-top instrument for performing protein characterization in microliter amounts of sample. Utilizing label-free…
Presentations
The Octet and Blitz Systems: Obtaining Kinetics From Crude Media
The Octet and Blitz Systems: Obtaining Kinetics From Crude Media
We have seen many examples of how the Octet systems can measure concentrations directly from crude samples.
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Presentations
Antibody investigations with immobilised HIV antigen and Fc receptors
Antibody investigations with immobilised HIV antigen and Fc receptors
We are a group of around 28 in the Department of Medicine, Division of Infectious Disease based at St Mary’s Hospital in Paddington.
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Presentations
Application of Label Free Technology for Biologics Discovery
Application of Label Free Technology for Biologics Discovery
Global biologics research and development arm of AstraZeneca Approximately 2,500 full-time employees in the US and UK Robust pipeline of more than 120 biologics in research and developm…
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Presentations
In Vitro Assay to Predict Human IgG Half-Life
In Vitro Assay to Predict Human IgG Half-Life
For therapeutic mAbs, longer half-life enhances: Safety Efficacy Cost Convenience For diagnostic mAbs, shorter half-life is usually desired. Lack of cost effective, high-throughput ass…
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Presentations
BLI Technology to Assess Quality and Compatibility of Commercial Antibodies from Multiple Vendors
BLI Technology to Assess Quality and Compatibility of Commercial Antibodies from Multiple Vendors
Founded in 2013 and resident of the Marcus Institute of Nanotechnology at Georgia Institutes of Technology in Atlanta Annular’s research team has over thirty years combined experience i…
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Presentations
Antigen and antibody development for IVD immunoassay using Octet Biolayer Interferometry
Antigen and antibody development for IVD immunoassay using Octet Biolayer Interferometry
Viral, bacterial and parasitological culture and purification Synthetic peptides and recombinant antigen production and purification Rabbit, goat, guinea pig and lamb polyclonal antibod…
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Presentations
Bio-Layer Interferometry: Reporting Kinetic Effects of Small Ligands on Protein-Protein Interactions
Bio-Layer Interferometry: Reporting Kinetic Effects of Small Ligands on Protein-Protein Interactions
Bacterial system as a model for studying all F-type ATP synthases simplest subunit composition all subunits have homologs in mitochondrial complex best system for genetic, mutagenic stu…
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Presentations
A Method to Determine the Reversibility of Inhibition for Glutamate Carboxypeptidase II Inhibitors using BioLayer Interferometry
A Method to Determine the Reversibility of Inhibition for Glutamate Carboxypeptidase II Inhibitors using BioLayer Interferometry
Most commonly diagnosed cancer among US men and accounts for the second leading cause of death
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Presentations
Fc-receptor Binding Assays
Fc-receptor Binding Assays
Fc receptors belong to the immunoglobulin superfamily and bind to the Fc-part of antibodies.
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Presentations
Getting the Best Data from your BLItz™ Label-Free System
Getting the Best Data from your BLItz™ Label-Free System
Bio-Layer Interferometry (BLI) Light is emitted from a lamp and directed through a biosensor. Reflected light is transmitted back to a spectrophotometer. As molecules bind to the tip of…
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Presentations
Bio-Layer Interferometry Technology Applied to Fragment Screening
Bio-Layer Interferometry Technology Applied to Fragment Screening
Most of human proteins do not work on their own but in complex with other protein(s), PPIs are central to all the biological processes and often dysregulated in disease – the possibilit…
Technical Guide
Anti-Human Fab-CH1 Biosensor Kinetics Assays
Anti-Human Fab-CH1 Biosensor Kinetics Assays
The Anti-Human Fab-CH1 Biosensor consists of a high-specificity anti-Human Fab-CH1 antibody fragment pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ sy…
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Technical Guide
Anti-Human Fab-CH1 (FAB) Biosensor Quantitation Assays
Anti-Human Fab-CH1 (FAB) Biosensor Quantitation Assays
The Anti-Human Fab-CH1 Biosensor consists of a high affinity antihuman CH1 affinity ligand pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ systems, the…
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Technical Guide
Biotinylate antibody from stock which contains carrier protein
Biotinylate antibody from stock which contains carrier protein
Biotinylate antibody from stock which contains carrier protein (Recommended procedure for use with FortéBio streptavidin biosensors)
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Technical Guide
Octet kinetics assay: method development guideline
Octet kinetics assay: method development guideline
The purpose of this document is to describe the procedure for developing a ligand binding kinetics assay on Octet® QKe, RED96, RED96e, RED384 and HTX instruments. The procedures describ…
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Technical Guide
Enabling Depth Filter Selection with Rapid Detection of Monoclonal Antibodies in Culture Using the BLItz System
Enabling Depth Filter Selection with Rapid Detection of Monoclonal Antibodies in Culture Using the BLItz System
Harvesting and recovering monoclonal antibodies (mAb) from mammalian cell culture bioreactor samples is just the first step of a closely coordinated chain of purification and processing…
Publications
Conserved Role of an N-Linked Glycan on the Surface Antigen of Human Immunodeficiency Virus Type 1 Modulating Virus Sensitivity to Broadly Neutralizing Antibodies against the Receptor and Coreceptor Binding Sites
Conserved Role of an N-Linked Glycan on the Surface Antigen of Human Immunodeficiency Virus Type 1 Modulating Virus Sensitivity to Broadly Neutralizing Antibodies against the Receptor and Coreceptor Binding Sites
Earlier studies have reported that the removal of a potential N-linked glycan (PNLG) site at amino acid residue 197 (N7) in the conserved region of gp120 of HIV-1 envelope glycoprotein…
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Publications
The Structural Basis of DNA Binding by the Single-stranded DNA-binding Protein from Sulfolobus Solfataricus
The Structural Basis of DNA Binding by the Single-stranded DNA-binding Protein from Sulfolobus Solfataricus
Single-stranded DNA-binding proteins (SSBs) are a class of proteins that are present in all organisms. SSBs are known to have one or more characteristic conserved oligonucleotide/oligos…
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Publications
A Search for Synergy in the Binding Kinetics of Trastuzumab and Pertuzumab Whole and F(ab) to Her2
A Search for Synergy in the Binding Kinetics of Trastuzumab and Pertuzumab Whole and F(ab) to Her2
It has been reported that the survival rate of Her2 overexpressing breast cancer patients can be improved significantly by combining the antibodies Trastuzumab and Pertuzumab in their t…
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Publications
Antibody Mimetic Receptor Proteins for Label-Free Biosensors
Antibody Mimetic Receptor Proteins for Label-Free Biosensors
The authors describe a detailed characterization of a model receptor protein based on a non-antibody scaffold protein (which binds to anti-myc tag antibody) as the target specific recep…
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Publications
Bacterial Cytoplasmic Display Platform Retained Display (ReD) Identifies Stable Human Germline Antibody Frameworks
Bacterial Cytoplasmic Display Platform Retained Display (ReD) Identifies Stable Human Germline Antibody Frameworks
Discussed in this article is the development of a novel cytoplasmic display platform namely, the Retained Display (ReD). The methodology eliminates certain difficulties inherent to conv…
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Publications
Characterization and Epitope Mapping of the Polyclonal Antibody Repertoire Elicited by Ricin Holotoxin-based Vaccination
Characterization and Epitope Mapping of the Polyclonal Antibody Repertoire Elicited by Ricin Holotoxin-based Vaccination
This is an investigation to obtain information and a plausible strategy for the development of an improved holotoxin-based vaccine against ricin. Ricin is one of the most lethal toxins…
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Publications
Crystal Structure of the Hendra Virus Attachment G Glycoprotein Bound to a Potent Cross-reactive Neutralizing Human Monoclonal Antibody
Crystal Structure of the Hendra Virus Attachment G Glycoprotein Bound to a Potent Cross-reactive Neutralizing Human Monoclonal Antibody
This study reports the crystal structure of the complex of Hendra Virus G (HeV-G) glycoprotein with human mAb m102.3, an m102.4 derivative, and describes NiV and HeV escape mutants. Bin…
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Publications
Enhancement of Tumor-specific T Cell-mediated Immunity in Dendritic Cell-based Vaccines by Mycobacterium Tuberculosis Heat Shock Protein X
Enhancement of Tumor-specific T Cell-mediated Immunity in Dendritic Cell-based Vaccines by Mycobacterium Tuberculosis Heat Shock Protein X
This report details the use of Heat Shock Protein (Hsp) X from Mycobacterium tuberculosis as a potent and strongly selective agonist for CTLs and is highly effective in the induction of…
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Publications
Following Natures Lead: On the Construction of Membrane-Inserted Toxins in Lipid Bilayer Nanodiscs
Following Natures Lead: On the Construction of Membrane-Inserted Toxins in Lipid Bilayer Nanodiscs
The article highlights the use of BLI (or the BLItz system in particular) to demonstrate important prerequisites such as the immobilization, orientation, the state of folding (unfolding…
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Publications
HIV-1 Receptor Binding Site-directed Antibodies Using a VH1-2 Gene Segment Orthologue are Activated by Env Trimer Immunization
HIV-1 Receptor Binding Site-directed Antibodies Using a VH1-2 Gene Segment Orthologue are Activated by Env Trimer Immunization
This investigation aims at finding possible explanations for the lack of broadly neutralizing activity in broadly neutralizing antibodies (bNAbs) following vaccination. They isolated a…
Videos and Demos
Flexible Monitoring of Small Molecules with Aptamer Affinity Reagents Using Bio-Layer Interferometry
Kinetics on the Octet Systems What Lies Beneath the Curves
Protein Concentration Measurements in Crude Samples in a Dip and Read Format
Using the BLItz Personal Assay System to Overcome Bioprocess Development Bottleneck
Related Products & Services of personal assay system – BLItz System
Easily Determine Interactions Kinetics
Get rate and affinity constants for binding interactions (ka, kd, KD) in minutes using only 4 µL of…
Read More
Instantly Detect Presence of Specific Protein
Detect the presence or absence of active proteins in just a drop of sample, and easily identify…
Read More
Rapidly Quantify your Protein with Confidence
Measure protein concentration without labeling or secondary reagents — even in crude media. With a…
Read MoreReady to get started?
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