Personal assay BLItz System
Label-free assays in a drop
Drop. Read. Done.
The BLItz® System brings label-free analysis to individual researchers, and is affordably priced so everyone can have one. With this personal assay system, you can run protein quantitation and kinetics experiments in only 4 µL of sample—right at your own lab bench.
-
Simple and rapid
The BLItz System enables the detection of specific mAbs, even in unpurified samples, in as little as 30 seconds.
-
Save sample
Affinity, concentration, and binding kinetics can be measured right at the bench in a 4 µL drop of sample.
Label-Free Analysis on BLItz System
Features
-
Instant protein presence
Detect the presence or absence of active proteins in just a drop of sample, and easily identify specific proteins in complex solutions. Data from the BLItz System complements existing methods like blots and gels, and its real-time binding curves give instant visual confirmation.
-
Faster protein quantitation
Measure protein concentration without labeling or secondary reagents — even in crude media. With a wide dynamic range and sensitivity down to a few ng/mL, the BLItz System gives you accurate quantitation in seconds compared to traditional methods that can often take hours.
-
Easy reagent QC testing
Rapidly identifying specific proteins with immunoassay-based techniques like ELISA or HPLC add many hours to the process and delay results. With the BLItz System, proteins and antibodies can be quantitated in a matter of seconds with high specificity and sensitivity, even in crude samples.
-
Effortless kinetics
Get rate and affinity constants for binding interactions (ka, kd, KD) in minutes using only 4µL of sample. With both a small price and a small size, the BLItz System can be your own personal label-free kinetics system. Ready to go right out of the box, its simple workflows let you run assays right at your lab bench with ease.
-
Smarter assay development
Monitoring the effects of changing conditions on binding interactions in real time, the BLItz System lets you develop better immunoassays in only a few minutes. Find the best antibody pairs, identify the best assay parameters, and even build and optimize an ELISA step by step.
-
Application Note
Best practices for performing quantitation assays using the BLItz System
Best practices for performing quantitation assays using the BLItz System
The BLItz® system provides a simple, rapid Dip and Read™ approach for protein quantitation in a small, affordably priced personal assay system. With BLItz, proteins and antibodies can be…
-
Application Note
Instant Determination of Protein Presence Using the BLItz System
Instant Determination of Protein Presence Using the BLItz System
The BLItz system provides a simple, rapid Dip and Read™ approach to protein analysis in an affordably priced personal assay system. Protein and antibody detection can be performed in a…
Applications for personal assay system – BLItz System
-
Easily Determine Interactions Kinetics
Get rate and affinity constants for binding interactions (ka, kd, KD) in minutes using only 4 µL of sample. With both a small price and a small size, the BLItz system can be your own personal label-free kinetics system. Ready to go right out of the box, its simple workflows let you run assays right at your lab bench with ease.
-
Rapidly Quantify your Protein with Confidence
Measure protein concentration without labeling or secondary reagents — even in crude media. With a wide dynamic range and sensitivity down to a few ng/mL, the BLItz system gives you accurate quantitation in seconds compared to traditional methods that can often take hours.
-
Instantly Detect Presence of Specific Protein
Detect the presence or absence of active proteins in just a drop of sample, and easily identify specific proteins in complex solutions. Data from the BLItz system complements existing methods like blots and gels, and its real-time binding curves give instant visual confirmation.
Resources for personal assay system – BLItz System
-
Application Note
Best practices for performing quantitation assays using the BLItz System
Best practices for performing quantitation assays using the BLItz System
The BLItz® system provides a simple, rapid Dip and Read™ approach for protein quantitation in a small, affordably priced personal assay system. With BLItz, proteins and antibodies can…
-
Application Note
Instant Determination of Protein Presence Using the BLItz System
Instant Determination of Protein Presence Using the BLItz System
The BLItz system provides a simple, rapid Dip and Read™ approach to protein analysis in an affordably priced personal assay system. Protein and antibody detection can be performed in a…
-
Application Note
Fast Quantitation of Proteins and Antibodies Using the BLItz System
Fast Quantitation of Proteins and Antibodies Using the BLItz System
Traditional techniques for determining concentration of a target protein such as ELISA and HPLC are both elaborate and timeconsuming, especially when analyzing complex matrices. For…
Data Sheet
Qualification and Compliance Tools for the BLItz® System
Qualification and Compliance Tools for the BLItz® System
Affordably priced and easy to use, the BLItz system is enabling labelfree detection and analysis across protein manufacturing, purification, and processing workflows. To support require…
Brochure
BLItz Label-free Assay in a Drop
BLItz Label-free Assay in a Drop
The BLItz® system finally makes label- free analysis available to individual researchers. Priced so everyone can have one, you can now monitor protein binding interactions, quantify pro…
Scientific Poster
Label-free Analysis from Microliter Amounts of Sample
Label-free Analysis from Microliter Amounts of Sample
BLItz instrument – A need was identified for an easy to use, compact, bench-top instrument for performing protein characterization in microliter amounts of sample. Utilizing label-free…
Presentations
The Octet and Blitz Systems: Obtaining Kinetics From Crude Media
The Octet and Blitz Systems: Obtaining Kinetics From Crude Media
We have seen many examples of how the Octet systems can measure concentrations directly from crude samples.
-
Presentations
Antibody investigations with immobilised HIV antigen and Fc receptors
Antibody investigations with immobilised HIV antigen and Fc receptors
We are a group of around 28 in the Department of Medicine, Division of Infectious Disease based at St Mary’s Hospital in Paddington.
-
Presentations
Application of Label Free Technology for Biologics Discovery
Application of Label Free Technology for Biologics Discovery
Global biologics research and development arm of AstraZeneca Approximately 2,500 full-time employees in the US and UK Robust pipeline of more than 120 biologics in research and developm…
-
Presentations
In Vitro Assay to Predict Human IgG Half-Life
In Vitro Assay to Predict Human IgG Half-Life
For therapeutic mAbs, longer half-life enhances: Safety Efficacy Cost Convenience For diagnostic mAbs, shorter half-life is usually desired. Lack of cost effective, high-throughput ass…
-
Presentations
BLI Technology to Assess Quality and Compatibility of Commercial Antibodies from Multiple Vendors
BLI Technology to Assess Quality and Compatibility of Commercial Antibodies from Multiple Vendors
Founded in 2013 and resident of the Marcus Institute of Nanotechnology at Georgia Institutes of Technology in Atlanta Annular’s research team has over thirty years combined experience i…
-
Presentations
Antigen and antibody development for IVD immunoassay using Octet Biolayer Interferometry
Antigen and antibody development for IVD immunoassay using Octet Biolayer Interferometry
Viral, bacterial and parasitological culture and purification Synthetic peptides and recombinant antigen production and purification Rabbit, goat, guinea pig and lamb polyclonal antibod…
-
Presentations
Bio-Layer Interferometry: Reporting Kinetic Effects of Small Ligands on Protein-Protein Interactions
Bio-Layer Interferometry: Reporting Kinetic Effects of Small Ligands on Protein-Protein Interactions
Bacterial system as a model for studying all F-type ATP synthases simplest subunit composition all subunits have homologs in mitochondrial complex best system for genetic, mutagenic stu…
-
Presentations
A Method to Determine the Reversibility of Inhibition for Glutamate Carboxypeptidase II Inhibitors using BioLayer Interferometry
A Method to Determine the Reversibility of Inhibition for Glutamate Carboxypeptidase II Inhibitors using BioLayer Interferometry
Most commonly diagnosed cancer among US men and accounts for the second leading cause of death
-
Presentations
Fc-receptor Binding Assays
Fc-receptor Binding Assays
Fc receptors belong to the immunoglobulin superfamily and bind to the Fc-part of antibodies.
-
Presentations
Getting the Best Data from your BLItz™ Label-Free System
Getting the Best Data from your BLItz™ Label-Free System
Bio-Layer Interferometry (BLI) Light is emitted from a lamp and directed through a biosensor. Reflected light is transmitted back to a spectrophotometer. As molecules bind to the tip of…
-
Presentations
Bio-Layer Interferometry Technology Applied to Fragment Screening
Bio-Layer Interferometry Technology Applied to Fragment Screening
Most of human proteins do not work on their own but in complex with other protein(s), PPIs are central to all the biological processes and often dysregulated in disease – the possibilit…
Technical Guide
Anti-Human Fab-CH1 Biosensor Kinetics Assays
Anti-Human Fab-CH1 Biosensor Kinetics Assays
The Anti-Human Fab-CH1 Biosensor consists of a high-specificity anti-Human Fab-CH1 antibody fragment pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ sy…
-
Technical Guide
Anti-Human Fab-CH1 (FAB) Biosensor Quantitation Assays
Anti-Human Fab-CH1 (FAB) Biosensor Quantitation Assays
The Anti-Human Fab-CH1 Biosensor consists of a high affinity antihuman CH1 affinity ligand pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ systems, the…
-
Technical Guide
Biotinylate antibody from stock which contains carrier protein
Biotinylate antibody from stock which contains carrier protein
Biotinylate antibody from stock which contains carrier protein (Recommended procedure for use with FortéBio streptavidin biosensors)
-
Technical Guide
Octet kinetics assay: method development guideline
Octet kinetics assay: method development guideline
The purpose of this document is to describe the procedure for developing a ligand binding kinetics assay on Octet® QKe, RED96, RED96e, RED384 and HTX instruments. The procedures describ…
-
-
Technical Guide
Enabling Depth Filter Selection with Rapid Detection of Monoclonal Antibodies in Culture Using the BLItz System
Enabling Depth Filter Selection with Rapid Detection of Monoclonal Antibodies in Culture Using the BLItz System
Harvesting and recovering monoclonal antibodies (mAb) from mammalian cell culture bioreactor samples is just the first step of a closely coordinated chain of purification and processing…
Publications
Conserved Role of an N-Linked Glycan on the Surface Antigen of Human Immunodeficiency Virus Type 1 Modulating Virus Sensitivity to Broadly Neutralizing Antibodies against the Receptor and Coreceptor Binding Sites
Conserved Role of an N-Linked Glycan on the Surface Antigen of Human Immunodeficiency Virus Type 1 Modulating Virus Sensitivity to Broadly Neutralizing Antibodies against the Receptor and Coreceptor Binding Sites
Earlier studies have reported that the removal of a potential N-linked glycan (PNLG) site at amino acid residue 197 (N7) in the conserved region of gp120 of HIV-1 envelope glycoprotein…
-
Publications
The Structural Basis of DNA Binding by the Single-stranded DNA-binding Protein from Sulfolobus Solfataricus
The Structural Basis of DNA Binding by the Single-stranded DNA-binding Protein from Sulfolobus Solfataricus
Single-stranded DNA-binding proteins (SSBs) are a class of proteins that are present in all organisms. SSBs are known to have one or more characteristic conserved oligonucleotide/oligos…
-
Publications
A Multidimensional Analytical Comparison of Remicade and the Biosimilar Remsima
A Multidimensional Analytical Comparison of Remicade and the Biosimilar Remsima
Biosimilars are biological drugs that are “equivalent” with regard to quality, efficacy, and safety to an original innovator product. Due to their low market price, biosimilars are mu…
-
Publications
A von Willebrand Factor Fragment Containing the D'D3 Domains is Sufficient to Stabilize Coagulation Factor VIII in Mice
A von Willebrand Factor Fragment Containing the D'D3 Domains is Sufficient to Stabilize Coagulation Factor VIII in Mice
This article reports the effect of a series of von Willebrand factor (VWF) fragments on plasma factor VIII (FVIII) binding in vitro as well as their effect in vivo using mouse models. T…
-
Publications
Application of the Log-normal Model for Long Term High Affinity Antibody/Antigen Interactions using Bio-Layer Interferometry
Application of the Log-normal Model for Long Term High Affinity Antibody/Antigen Interactions using Bio-Layer Interferometry
Optical biosensors are commonly used to characterize biomolecular interactions and are an important tool for quality control and process development groups. In this report, the author…
-
Publications
Bio-Layer Interferometry of a Multivalent Sulfated Virus Nanoparticle with Heparin-Like Anticoagulant Activity
Bio-Layer Interferometry of a Multivalent Sulfated Virus Nanoparticle with Heparin-Like Anticoagulant Activity
With the intention of finding an alternative to heparin, the authors investigated the feasibility of sulfated virus-like nanoparticles (sulf-VLP) using Pall ForteBio BLItz platform in t…
-
Publications
Chemical Biology. A bump-and-hole Approach to Engineer Controlled Selectivity of BET Bromodomain Chemical Probes
Chemical Biology. A bump-and-hole Approach to Engineer Controlled Selectivity of BET Bromodomain Chemical Probes
The article describes an approach to obtain selective inhibitors for Bromo and Extra-terminal (BET) bromodomain proteins. The BET proteins are important targets due to their roles in tr…
-
Publications
Cyclic di-GMP Sensing via the Innate Immune Signaling Protein STING
Cyclic di-GMP Sensing via the Innate Immune Signaling Protein STING
The structural characteristics of STING (stimulator of interferon genes) cytosolic domain are investigated in this publication. The BLItz system was used to study binding between STING…
-
Publications
Envelope Variants Circulating as Initial Neutralization Breadth Developed in Two HIV-infected Subjects Stimulate Multiclade Neutralizing Antibodies in Rabbits
Envelope Variants Circulating as Initial Neutralization Breadth Developed in Two HIV-infected Subjects Stimulate Multiclade Neutralizing Antibodies in Rabbits
Based on the hypothesis that the B cells are developing broad antibodies by exposure to ever evolving viral envelope population, the authors tested the ability to use multiple envelops…
-
Publications
Glypican-3-targeting F(ab')2 for 89Zr PET of Hepatocellular Carcinoma
Glypican-3-targeting F(ab')2 for 89Zr PET of Hepatocellular Carcinoma
This article reports the development of a 89Zr PET imaging probe that only uses the F(ab')2 region of anti GPC3 mAb (αGPC3) to address several limitations shown by the whole-αGPC3 targe…
Related Products & Services of personal assay system – BLItz System
Easily Determine Interactions Kinetics
Get rate and affinity constants for binding interactions (ka, kd, KD) in minutes using only 4 µL of…
Read More
Instantly Detect Presence of Specific Protein
Detect the presence or absence of active proteins in just a drop of sample, and easily identify…
Read More
Rapidly Quantify your Protein with Confidence
Measure protein concentration without labeling or secondary reagents — even in crude media. With a…
Read MoreReady to get started?
We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.