Affordability and unmatched assay performance in filter-based microplate readers

 

The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements.

  • Get great performance with LEDs

    Get great performance with LEDs

    The readers employ patented high-power light emitting diodes (LED) that are high powered at critical wavelengths, with an optimized light source for a specific dye or application.

  • Count single photons

    Count single photons

    The photomultiplier tube (PMT) detector operates in photon counting mode, affording increased sensitivity for red dyes with a linear dynamic range of over six orders of magnitude.

  • Get tailored data analysis

    Get tailored data analysis

    Save time from exporting data to external applications. SoftMax Pro Software provides more than 160 predefined yet powerful experiment protocols and programming capabilities for quick data analysis.

Features

  • Visible absorbance

    This reader provides visible absorbance, top-reading fluorescence intensity, and glow luminescence modes in 96- to 384-well plates with wavelength selection options from 340-650 nm.

  • UV and visible absorbance

    This reader provides UV and visible absorbance, top and bottom fluorescence intensity, glow luminescence, FP, and TRF modes in 6- to 1536-well plates with wavelength selection options from 230-750 nm.

Latest Resources

9

Applications of FilterMax F3 and F5 Multi-Mode Microplate Readers

  • Absorbance

    Absorbance

    Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

    Learn more 

    Cell viability

    Cell viability

    Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones can be characterized on the CloneSelect Imager and the SpectraMax i3x microplate reader and imaging cytometer.

  • ELISA

    Elisa

    Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

    Learn more 

    Fluorescence

    Fluorescence

    Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. We also cover many fluorescence-based assays including cell viability, GPCR activity, and fluorescent nucleic acid quantification.

    Learn more  

  • Fluorescence Polarization (FP)

    Fluorescence Polarization

    Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

    Learn more  

    Fluorescent Protein Detection

    Fluorescent Protein Detection

    Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.

    Read Application Note 

  • Luminescence

    Luminescence

    Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

    Learn more  

    Mycoplasma Monitoring

    Mycoplasma Monitoring

    Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Symptoms of mycoplasma contamination include a reduction in the rate of proliferation and changes in cellular responses, including gene expression. Mycoplasma cannot be detected by simply examining cell cultures under a microscope, so a variety of methods such as fluorescence- and luminescence-based assays have been developed to enable researchers to monitor for contamination.

    Read Application Note 

  • TRF, TR-FRET & HTRF

    Time-resolved

    Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

    Learn more  

Specifications & Options of FilterMax F3 and F5 Multi-Mode Microplate Readers

 

*Using lowest settings and speed read when available.

Resources of FilterMax F3 and F5 Multi-Mode Microplate Readers

Presentations
Videos & Webinars
Expanding Assay Applications with Multi-Mode Readers

Expanding Assay Applications with Multi-Mode Readers from ELISA to AlphaScreen

  • Citation
    Dated: Dec 15, 2020
    Publication Name: ACM SIGPLAN Notices

    Data flow fusion with series expressions in Haskell

    Existing approaches to array fusion can deal with straight-line producer consumer pipelines, but cannot fuse branching data flows where a generated array is consumed by several different consumers. Branching data flows are common and natural to write, but a lack of fusion leads to the creation of an intermediate array at every branch point. We… View more

    Existing approaches to array fusion can deal with straight-line producer consumer pipelines, but cannot fuse branching data flows where a generated array is consumed by several different consumers. Branching data flows are common and natural to write, but a lack of fusion leads to the creation of an intermediate array at every branch point. We present a new array fusion system that handles branches, based on Waters's series expression framework, but extended to work in a functional setting. Our system also solves a related problem in stream fusion, namely the introduction of duplicate loop counters. We demonstrate speedup over existing fusion systems for several key examples.

    Contributors: Ben Lippmeier, Manuel M T Chakravarty, Gabriele Keller, Amos Robinson

     
    Go to article

  • Citation
    Dated: Dec 29, 2013
    Publication Name: Molecular and Cellular Biochemistry volume

    Activity of imidazole compounds on Leishmania (L.) infantum chagasi: reactive oxygen species induced by econazole

    Drug repositioning has been considered a promising approach to discover novel treatments against neglected diseases. Among the major protozoan diseases, leishmaniasis remains a public health threat with few therapeutic alternatives, affecting 12 million people in 98 countries. In this study, we report the in vitro antileishmanial activity of the… View more

    Drug repositioning has been considered a promising approach to discover novel treatments against neglected diseases. Among the major protozoan diseases, leishmaniasis remains a public health threat with few therapeutic alternatives, affecting 12 million people in 98 countries. In this study, we report the in vitro antileishmanial activity of the imidazole drugs clotrimazole, and for the first time in literature, econazole and bifonazole and their potential action to affect the regulation of reactive oxygen species (ROS) of the parasites. The lethal action of the imidazoles was investigated using spectrofluorimetric techniques to detect ROS content, plasma membrane permeability, and mitochondrial membrane potential. The imidazoles showed activity against L.

    Contributors: Juliana Tonini Mesquita, Thais Alves da Costa-Silva, Samanta Etel Treiger Borborema & André Gustavo Tempone  
    Go to article

  • Citation
    Dated: Dec 15, 2013
    Publication Name: Phytomedicine

    Butenolides from Nectandra oppositifolia (Lauraceae) displayed anti-Trypanosoma cruzi activity via deregulation of mitochondria

    From a previous screening of Brazilian biodiversity for antitrypanosomal activity, the n-hexane extract from twigs of Nectandra oppositifolia (Lauraceae) demonstrated in vitro activity against Trypanosoma cruzi. View more

    From a previous screening of Brazilian biodiversity for antitrypanosomal activity, the n-hexane extract from twigs of Nectandra oppositifolia (Lauraceae) demonstrated in vitro activity against Trypanosoma cruzi.

    Contributors: Geanne A. AlvesConservaa1, Thais A.da Costa-Silvaa1, MaiaraAmaralb, Guilherme M.Antarc, Bruno J.Nevesde, Carolina H.Andradee, Andre G.Temponeb João Henrique G.Lago  
    Go to article

FilterMax F3 and F5 Multi-Mode Microplate Readers

Product

Product Number

FilterMax F3 Microplate Reader F3
FilterMax F5 Microplate Reader F5
FilterMax FLUORESCENCE EXCITATION FILTER 360NM BW 35NM
6590-0042
FilterMax FLUORESCENCE EXCITATION FILTER 485NM BW 20NM
6590-0043
FilterMax FLUORESCENCE EXCITATION FILTER 535NM BW 25NM
6590-0044
FilterMax FLUORESCENCE EXCITATION FILTER 430NM BW 35NM
6590-0045
FilterMax FLUORESCENCE EXCITATION FILTER 590NM BW 20NM
6590-0046
FilterMax FLUORESCENCE EXCITATION FILTER 320NM BW 25NM
6590-0047
FilterMax FLUORESCENCE EXCITATION FILTER 405 NM BW 30NM
6590-0048
FilterMax FLUORESCENCE EXCITATION FILTER 465NM BW 35NM
6590-0049
FilterMax FLUORESCENCE EXCITATION FILTER 500NM BW 25NM
6590-0050
FilterMax FLUORESCENCE EXCITATION FILTER 518NM BW 26NM
6590-0051
FilterMax FLUORESCENCE EXCITATION FILTER 525 NM BW 45NM
6590-0052
FilterMax FLUORESCENCE EXCITATION FILTER 540NM BW 25NM
6590-0053
FilterMax FLUORESCENCE EXCITATION FILTER 550NM BW 10 NM
6590-0054
FilterMax FLUORESCENCE EXCITATION FILTER 585NM BW 10NM                                               
6590-0055
 
Fluorescence (FI) Emission Filters
 
Description
 
FilterMax FLUORESCENCE EMISSION FILTER 465NM BW 35NM
6590-0056
FilterMax FLUORESCENCE EMISSION FILTER 535NM BW 25NM
6590-0057
FilterMax FLUORESCENCE EMISSION FILTER 595NM BW 35NM
6590-0058
FilterMax FLUORESCENCE EMISSION FILTER 625NM BW 35NM
6590-0059
FilterMax FLUORESCENCE EMISSION FILTER 430NM BW 35NM
6590-0060
FilterMax FLUORESCENCE EMISSION FILTER 650NM BW 40NM
6590-0061
FilterMax FLUORESCENCE EMISSION FILTER 485NM BW 20NM
6590-0062
FilterMax FLUORESCENCE EMISSION FILTER 500 NM BW 25NM
6590-0063
FilterMax FLUORESCENCE EMISSION FILTER 550NM BW 10NM
6590-0064
FilterMax FLUORESCENCE EMISSION FILTER 574 NM BW 25NM
6590-0065
FilterMax FLUORESCENCE EMISSION FILTER 585NM BW 10NM
6590-0066
FilterMax FLUORESCENCE EMISSION FILTER 635NM BW 35NM
6590-0067
FilterMax FLUORESCENCE EMISSION FILTER 660NM BW 32NM
6590-0068
FilterMax FLUORESCENCE EMISSION FILTER 668NM BW 12NM
6590-0069
FilterMax FLUORESCENCE EMISSION FILTER 670NM BW 40NM
6590-0070
 
Fluorescence Polarization (FP) Filters
 
Product Name
Product Number
FilterMax FLUORESCENCE POLARIZATION (FP) Excitation Filter Visible                               6590-0071
FilterMax FLUORESCENCE POLARIZATION (FP) Emission Filter Visible (QTY 2)
6590-0072
 
 Time-Resolved Fluorescence (TRF) Filters
 
Product Name
Product Number
FilterMax TIME RESOLVED FLUORESCENCE (TRF) Excitation Filter 370NM BW80 NM                         
6590-0073
 
 Absorbance Filters
 
Product Name
Product Number
FilterMax ABSORBANCE FILETER 280NM BW 5,5NM 6590-002
FilterMax ABSORBANCE FILTER 260NM BW 5,5NM 6590-001
FilterMax ABSORBANCE FILTER 320NM BW 5,5NM
6590-0003
FilterMax ABSORBANCE FILTER 420NM BW 8NM
6590-0004
FilterMax ABSORBANCE FILTER 450NM BW 8NM
6590-0005
FilterMax ABSORBANCE FILTER 465NM BW 8NM
6590-0006
FilterMax ABSORBANCE FILTER 470NM BW 8NM
6590-0007
FilterMax ABSORBANCE FILTER 480NM BW 8NM
6590-0008
FilterMax ABSORBANCE FILTER 490NM BW 8NM
6590-0009
FilterMax ABSORBANCE FILTER 492NM BW 8NM
6590-0010
FilterMax ABSORBANCE FILTER 510NM BW 8NM
6590-0011
FilterMax ABSORBANCE FILTER 515NM BW 8NM
6590-0012
FilterMax ABSORBANCE FILTER 520NM BW 8NM
6590-0013
FilterMax ABSORBANCE FILTER 532NM BW 8NM
6590-0014
FilterMax ABSORBANCE FILTER 540NM BW 8NM
6590-0015
FilterMax ABSORBANCE FILTER 548NM BW 8NM
6590-0016
FilterMax ABSORBANCE FILTER 550NM BW 8NM
6590-0017
FilterMax ABSORBANCE FILTER 560NM BW 8NM
6590-0018
FilterMax ABSORBANCE FILTER 562NM BW 8NM
6590-0019
FilterMax ABSORBANCE FILTER 570NM BW 8NM
6590-0020
FilterMax ABSORBANCE FILTER 575NM BW 8NM
6590-0021
FilterMax ABSORBANCE FILTER 580NM BW 8NM
6590-0022
FilterMax ABSORBANCE FILTER 590NM BW 8NM
6590-0023
FilterMax ABSORBANCE FILTER 595NM BW 8NM
6590-0024
FilterMax ABSORBANCE FILTER 600NM BW 8NM
6590-0025
FilterMax ABSORBANCE FILTER 610NM BW 8NM
6590-0026
FilterMax ABSORBANCE FILTER 620NM BW 8NM
6590-0027
FilterMax ABSORBANCE FILTER 630NM BW 8NM
6590-0028
FilterMax ABSORBANCE FILTER 640NM BW 8NM
6590-0029
FilterMax ABSORBANCE FILTER 645NM BW 8NM
6590-0030
FilterMax ABSORBANCE FILTER 650NM BW 8NM
6590-0031
FilterMax ABSORBANCE FILTER 340NM BW 10NM
6590-0032
FilterMax ABSORBANCE FILTER 350NM BW 10NM
6590-0033
FilterMax ABSORBANCE FILTER 360NM BW 10NM
6590-0034
FilterMax ABSORBANCE FILTER 370NM BW 10NM
6590-0035
FilterMax ABSORBANCE FILTER 380NM BW 10NM
6590-0036
FilterMax ABSORBANCE FILTER 405NM BW 10NM
6590-0037
FilterMax ABSORBANCE FILTER 414NM BW 10NM
6590-0038
FilterMax ABSORBANCE FILTER 260NM BW 12NM
6590-0039
FilterMax ABSORBANCE FILTER 280NM BW 12NM
6590-0040
FilterMax ABSORBANCE FILTER 320NM BW 12NM
6590-0041

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