APPLICATION NOTE
AI-enabled automated compound screening for toxicity effects using healthy human intestinal organoids
Ai-enabled toxicity screening using human intestinal organoids
Discover how the CellXpress.ai® Automated Cell Culture System and the ImageXpress® HCS.ai High-Content Screening System deliver a fully automated, AI-powered workflow for compound toxicity screening using healthy human intestinal organoids.
This application note highlights a scalable solution that integrates automated organoid culture, compound dosing, and high-content imaging with machine learning–driven phenotypic analysis. By combining deep learning segmentation (SINAP) and phenotypic classification (Phenoglyphs™) found in IN Carta® Image Analysis Software, the system accurately distinguishes between cytotoxic, cytostatic, and viable organoid responses.
The result is a reproducible, high-throughput platform that enhances sensitivity, reduces manual handling, and provides a more physiologically relevant model for intestinal toxicity assessment.
