Intestinal Organoids
Intestinal organoids are 3D microtissue models that recapitulate structures in the intestinal lumen and on the surrounding intestinal epithelium.
Most intestinal organoids are derived from primary intestinal tissue cultured in Matrigel domes with growth factors. Under these conditions, the intestinal cells self-arrange to form a single epithelial layer. The epithelium contains the essential cell populations found in the adult intestinal epithelium and is arranged in a crypt-villus structure. It can simulate essential intestinal functions, such as nutrient absorption and mucus secretion.
The cell composition and arrangement of the epithelium make intestinal organoids useful for studying intestinal cell biology, regeneration, differentiation, as wells as diseases phenotypes including effects of specific mutations, microbiome, or inflammation process.
Automation of 3D intestinal organoid culture and imaging protocols
Automated imaging and analysis of organoids are important for quantitative assessment of phenotypic changes in organoids, and for increasing the throughput for experiments and tests.
Here we demonstrate a workflow for the automation of 3D intestinal organoid culture. We developed automated methods for the seeding, and media exchange, as well as monitoring the development of intestinal organoids. In addition, this method allows automation of compound testing and evaluation of phenotypic changes.
Watch our short webinar with Dr. Oksana Sirenko, senior scientist as she discusses the protocols used to automate 3D intestinal organoids.
Integrated system for high-throughput intestinal organoid workflow
The automated organoid workcell includes the ImageXpress® Confocal HT.ai High-Content Imaging System, automated CO2 incubator, automated Biomek i7 liquid handler, collaborative robot and rail, and additional, optional instruments including an automated centrifuge, our ImageXpress® Pico Automated Cell Imaging System, and SpectraMax microplate reader. This integrated system allows for automated monitoring, maintenance, and characterization of growth and differentiation of organoids and stem cells, as well as testing the effects of various compounds.
Our lab automation for high-throughput, high-content screening include scientists and engineers who can customize our instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. From incubators, liquid handlers, and robotics to customized software and hardware—and with over 35 years of experience in the life science industry—you can count on us to deliver quality products and provide worldwide support.
Featured asset
Intestinal organoids for automated screening assays. High content imaging and analysis of organoid morphology
In this application, 3D intestinal organoids were developed from primary mouse intestinal cells cultured in Matrigel. Methods for automated liquid handling were developed for cell seeding in Matrigel droplets and for media addition and exchanges. Six-week-old organoids were treated with various compounds followed by the live/dead assay and advanced image analysis to assess effects of treatment. In addition, methods to increase throughput, such as automated organoid assays and compound screening, are presented.
Latest resources on organoid research
The complexity of 3D assays remains a hurdle for the wider adoption of organoid models in research and drug screening. New advanced tools for imaging and analysis, as well as assay automation are critical for increased quality of information, throughput, and precision of complex biological models. Learn how high-throughput, high-content imaging and analysis paired with AI-based analytic tools can improve the accuracy of your organoid studies.
![3D organoids and automation of complex cell assays [Podcast]](/sites/default/files/2023-01/3d-organoids-and-automation-of-complex-cell-assays.jpg)
Resources for Intestinal Organoids
Blog
How 3D Cell Models Will Shape the Future of Drug Discovery
How 3D Cell Models Will Shape the Future of Drug Discovery
Target discovery and drug development rely heavily on 2D cell and animal models to decipher efficacy and toxic effect of drug candidates. Yet, 90% of candidates fail to make it past…
News
Molecular Devices inks deal with HUB Organoids to advance automated intestinal organoid screening technology
Molecular Devices inks deal with HUB Organoids to advance automated intestinal organoid screening technology
SAN JOSE, Calif., and UTRECHT, Netherlands, Feb. 13, 2023 – Molecular Devices, LLC., a leading provider of high-performance life science solutions, and HUB Organoids (HUB), the pioneer in th…
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Blog
A case study for assay-ready patient-derived organoids (PDOs) and high-throughput 3D imaging to advance drug discovery
A case study for assay-ready patient-derived organoids (PDOs) and high-throughput 3D imaging to advance drug discovery
Introduction – the problem. The average cost of bringing a new drug to the clinic is around $1 billion according to a study conducted by the London School of Economics in March 2020…
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Blog
3D organoids and automation of complex cell assays [Podcast]
3D organoids and automation of complex cell assays [Podcast]
As we enter the era of sophisticated drug discovery with gene therapy and personalized medicine, we need to be prepared to study complex diseases, assess the therapeutic effect of…
Publications
Improving Drug Development: Molecular Devices and Cellesce Aim to Advance the Use of Organoids
Improving Drug Development: Molecular Devices and Cellesce Aim to Advance the Use of Organoids
Following our acquisition of Cellesce Ltd, Tanya Samazan from Instrument Business Outlook dove deep into conversation with Molecular Devices President Susan Murphy and Cellesce CEO Vicky Mar…
eBook
Building complexity in organoid models
Building complexity in organoid models
Complex biological systems such as spheroids, organoids, and organ-on-a-chip are becoming more popular for disease modeling and drug screening as they better simulate organs and tissues comp…
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Blog
Advanced technology for automated 3D biology workflows #SLASEurope2022
Advanced technology for automated 3D biology workflows #SLASEurope2022
SLAS Europe 2022, hosted numerous sessions packed with the latest research on emerging topics as well as sessions and panel discussions focused on how to build, and succeed, in a…
Application Note
AI enabled phenotypic analysis of label-free intestinal organoids
AI enabled phenotypic analysis of label-free intestinal organoids
The intestinal crypt system is an important part of the intestinal organoid where mature organoids have more complex and numerous crypt structures. Yet, due to their high turnover rate and…
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Blog
Stem Cell Science and Regenerative Medicine – Technology and Methods Presented at ISSCR 2022
Stem Cell Science and Regenerative Medicine – Technology and Methods Presented at ISSCR 2022
It was another great year at ISSCR 2022! Leaders from across the globe came together to discuss new technologies, share insights, and explore the newest breakthroughs in stem cell…
Scientific Poster
Monitoring growth and characterization of compound effects in 3D intestinal organoids by high content imaging
Monitoring growth and characterization of compound effects in 3D intestinal organoids by high content imaging
3D cell models representing various tissues were successfully used for studying complex biological effects and tissue architecture, however, the complexity of 3D models remains a hurdle for…
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Blog
How automated organoid cell cultures are developed, imaged, and analyzed
How automated organoid cell cultures are developed, imaged, and analyzed
3D cell models are becoming increasingly popular for studying complex biological effects, tissue functionality, and diseases. Their ability to self-organize and mimic…
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eBook
The essential guide to organoids in drug discovery
The essential guide to organoids in drug discovery
Three-dimensional (3D) organoid development is one of the most important advancements in drug discovery research to date.
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Scientific Poster
Intestinal organoids for automated screening assays. High content imaging and analysis of organoid morphology
Intestinal organoids for automated screening assays. High content imaging and analysis of organoid morphology
Here we describe a workflow for automation of organoid culture. The automated method utilizes an integrated work-cell, consisting of several instruments providing automated cell culture, mon…
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Blog
Engineering Next-gen Organoids with Automated Lab Workflows at #SLAS2022
Engineering Next-gen Organoids with Automated Lab Workflows at #SLAS2022
SLAS2022, the Society for Lab Automation and Screening conference offered another exciting year for learning about innovative laboratory technologies. Whether you attended in-person…
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Scientific Poster
Automation of 3D organoid culture workflow with deep-learning based image analysis
Automation of 3D organoid culture workflow with deep-learning based image analysis
3D cell culture as a model system is increasingly popular because it recapitulates the in vivo microenvironment better than 2D cell cultures. Organoids have the capacity for stable different…
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Blog
History of Organoid Research: From Sponge Cells to Functional Organs
History of Organoid Research: From Sponge Cells to Functional Organs
Let's begin with a simple definition of an organoid which refers to a three-dimensional assembly that contains multiple cell types that are arranged with realistic histology, at…
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Application Note
Organoids for disease modeling and in vitro drug screening
Organoids for disease modeling and in vitro drug screening
We describe an automated integrated system that would allow automated monitoring, maintenance, and characterization of growth and differentiation of organoids and stem cells, as well as…
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Publications
Project Profile: Organoid Innovation Center
Project Profile: Organoid Innovation Center
Lab Manager speaks to Dan O’Connor, vice president, drug discovery, Molecular Devices, about the company’s Organoid Innovation Center in San Jose, CA. The center is 180 sq. ft., within a lar…
Customer Breakthrough
Bioneer use the ImageXpress Micro Confocal for high-throughput imaging of 3D disease models
Bioneer use the ImageXpress Micro Confocal for high-throughput imaging of 3D disease models
Revolutionizing early drug discovery for immuno-oncology and neurodegenerative disease modelling: High throughput imaging of 3D models of disease Bioneer A/S is a Danish research-based ser…
Videos & Webinars
The search for answers: Using lab automation with patient-derived tumoroids to find more relevant therapies for clinically aggressive cancers
Intestinal organoids for automated screening assays
Application and Analysis of Organoid Systems
Capturing the complexity of 3D biology: Organoids for disease modelling and toxicity research
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