Cardiotoxicity

3D cardiac spheroids

There is an increasing interest in exploring the use of three-dimensional (3D) spheroids for modeling tissue biology and toxicity assessment. The development of quantitative assays in higher throughput using 3D cultures is an active area of investigation. Studying the formation of 3D spheroids derived from human induced pluripotent stem cells (iPSC), using both high-content imaging (HCI) and fast kinetic fluorescence imaging helps measures the impact of various compounds on the beating rate and pattern of cardiac spheroids as monitored by changes in intracellular calcium levels with calcium-sensitive dyes.

• In Vitro Assessment of Drug Effects on Human iPSC-Derived Cardiac Spheroid Cultures
• Phenotypic Characterization of Compound Effects on iPSC-derived Cardiac & Liver Spheroids
• Assessment of drug effects on cardiomyocyte physiology using human iPSC-derived cardiac spheroids

Assessment of compound-induced pro-arrhythmic effects in vitro

Using human iPSC-derived cardiomyocytes as an in vitro model, we evaluated the responses and concentration dependence to 28 drugs linked to low, intermediate, and high torsades de pointes (TdP) risk categories. The impact of various compounds on the beating rates and patterns of cardiomyocyte spontaneous activity was monitored by changes in intracellular Ca2+ oscillations measured by fast kinetic fluorescence with calcium-sensitive dyes on the FLIPR Penta system.

• High-throughput assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocyte
• Multi-parametric assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes

Calcium oscillation

Induced pluripotent stem cell (iPSC)- derived cardiomyocytes are a particularly attractive in vitro model system due to their use for evaluation of compound effects on both cardiac function and safety. Calcium signal oscillation reflects changes in cytoplasmic calcium concentration making it possible to use a calcium sensitive dye such as the EarlyTox™ Cardiotoxicity Kit.

• Measure Calcium Oscillation in iCell Cardiomyocytes2 on the FLIPR Tetra System
• Explore cardiac function by measuring calcium oscillation or contraction patterns

Calcium oscillation in 3D neuronal spheroids

3D neural spheroids contain a neural network enriched in synapses, creating a highly functional neuronal circuitry that displays spontaneous, synchronized, readily detectable calcium oscillations.

• Phenotypic characterization of neuroactive compound effects
• Functional evaluation of neurotoxic and neuroactive compound effects in iPSC-derived 3D neural co-cultures

Cardiomyocyte assays

Stem cell-derived human cardiomyocytes have phenotypic characteristics and electrophysiological profiles similar to those of native human cardiac cells. Cardiomyocytes in culture are able to form a beating syncytium, which behaves similarly to native cardiomyocytes. Oscillation of intracellular calcium levels occurring with synchronized contractions of the cells can be monitored using a calcium-sensitive dye, and treatment-induced changes in the pattern of oscillation can be monitored via changes in fluorescent signal over time.

• Multiparametric assays for cardiomyocytes on a single platform
• Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
• Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
• Automated Functional Cellular Analyses of Human iPS-derived Cardiomyocytes

Cardiotoxicity assays

Cardiac toxicity is responsible for a large percentage of new drugs that fail in clinical trials. The development of highly predictive in vitro assays suitable for highthroughput screening is critical to improve the ineffeciencies and high costs associated with cardiac safety compound failure.

• High throughput cardiotoxicity assays using stem cell-derived cardiomyocytes
• Predictive Assays for High Throughput Assessment of Cardiac Toxicity and Drug Safety
• EarlyTox Cardiotoxicity Kit provides biologically relevant cardiotoxicity data earlier in the drug discovery process

eBooks

Enjoy a compilation of eBooks related to the real-time, kinetic high-throughput cellular assay screening solution, FLIPR system.

• Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
• Optimized Assays for Breakthrough Research
• Identify Targets Like a Pro

Publications on cardiotoxicity

Curated cardiotoxicity articles from Molecular Devices subject matter experts, featured in trade journals offered on pubmed.gov and Research Gate.

• Assessment of Beating Paramaters in Human Induced Pluripotent Stem Cells Enables Quantitative in Vitro Screening for Cadiotoxicity
• Phenotypic Assays for Characterizing Compound Effects on Induced Pluripotent Stem Cell-Derived Cardiac Spheroids
• In Vitro Cardiotoxicity Assessment of Environmental Chemicals Using an Organotypic Human Induced Pluripotent Stem Cell-Derived Model
• Multiparameter In Vitro Assessment of Compound Effects on Cardiomyocyte Physiology Using iPSC Cells

Toxicology

Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It is a growing concern in our world today as we are exposed to more and more chemicals, both in our environment and in the products we use.

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