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FLIPR Calcium Assay Kit

 

Built as the most comprehensive portfolio of calcium reagents, the kits deliver pre-optimized, homogeneous, fluorescence-based formulations to expedite assay development and screening of GPCR and ion channel targets. FLIPR Calcium Assay Kits provide the most comprehensive method for detecting intracellular calcium changes in a simple and reliable homogeneous assay format. Select from six formulations to complement your target of interest.

FLIPR Assay Kits employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits.

Key Assay Features and Benefits

  • Universal mix-and-read protocol accelerates assay workflow and increases throughput
  • Superior signal-to-noise ratio facilitates confirmation of endogenous or transiently transfected receptor activity during assay development
  • Pre-optimized and validated protocols for the FLIPR® Tetra and FlexStation® Systems ensure you can navigate both routine and unconventional cell lines and targets

FLIPR Calcium 6 and 6-QF Assay Kits

This exclusive fluorophore features the highest quantum yield of any calcium indicator on the market, delivering the greatest signal window available in a calcium assay kit. The substantial increase in signal amplitude helps to rekindle low signal screens; including endogenous, primary or stem cell targets. Additionally, the novel dye is unaffected by organic anion exchange proteins, enabling measurement with minimal to no probenecid present.

When utilized in the quench-free (QF) formulation, the dye is ideal for quench sensitive targets or assays requiring multiplexing of calcium-flux and luminescent assays within one well.

FLIPR Calcium 5 Assay Kit

A novel fluorophore that amplifies the assay signal window while leveraging the superior masking technology used in the FLIPR Calcium 4 Assay Kit to reduce background fluorescence. In addition to the above assays, receptors with low receptor expression and other targets that give a small calcium response are candidates for this kit.

FLIPR Calcium 4 Assay Kit

Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. It is more inert and reduces compound interference. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors.

Latest Resources

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Automated Monitoring of Development and Activity Analysis of iPSC-derived 3D Cerebral Organoids

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Automated Monitoring of Development and Activity Analysis of iPSC-derived 3D Cerebral Organoids

The human brain is highly complex which makes it challenging to study both in vitro and in-model organisms. Cultured neurons do not sufficiently recapitulate the three-…
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Optimized Assays for Breakthrough Research

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Optimized Assays for Breakthrough Research

Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.

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Validation of TRPV1 and ASIC1 Ligand-Gated Ion Channels using Automated Patch Clamp and FLIPR with novel Ca2+ detection dye

Scientific Poster

Validation of TRPV1 and ASIC1 Ligand-Gated Ion Channels using Automated Patch Clamp and FLIPR with novel Ca2+ detection dye

In one series of experiments, we constructed and validated cell based assays in which the FLIPR Calcium 6 assay kit containing this novel calcium fluorophore was used to…

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Optogenetics and the FLIPR: How to Use Light to Set up a Cav1.3 High-Throughput Screening Assay

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Optogenetics and the FLIPR: How to Use Light to Set up a Cav1.3 High-Throughput Screening Assay

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Optimizing a Transient Receptor Potential (TRP) Channel Assay on the FLIPRTETRA® Instrument using hTRPV1-HEK293 Cells and the FLIPR® Calcium 5 Assay Kit

Scientific Poster

Optimizing a Transient Receptor Potential (TRP) Channel Assay on the FLIPRTETRA® Instrument using hTRPV1-HEK293 Cells and the FLIPR® Calcium 5 Assay Kit

This PDF describes optimization of the TRP (transient receptor potential) channel assay on the FLIPR TETRA instrument using hTRPV1-HEK293 cells and the FLIPR calcium 5…

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Functional Characterisation of Recombinant Muscarinic M3 Receptors in Assay-Ready Frozen Cells using a Novel Calcium Assay Kit on the FLIPRTETRA® Instrument

Scientific Poster

Functional Characterisation of Recombinant Muscarinic M3 Receptors in Assay-Ready Frozen Cells using a Novel Calcium Assay Kit on the FLIPRTETRA® Instrument

This poster compares the performance of cryopreserved "frozen" cells vs. cells in culture when used for screening GPRC drug candidates on the FLIPR Tetra instrument.

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Cell Based Assays on the FLIPR TETRA® System : Comparison of the FLIPR Calcium 5 Assay Kit to Other Fluorescence Based Calcium Flux Assays

Scientific Poster

Cell Based Assays on the FLIPR TETRA® System : Comparison of the FLIPR Calcium 5 Assay Kit to Other Fluorescence Based Calcium Flux Assays

This poster explores FLIPR Calcium 5 Assay Kit containing a superior performance calcium-sensitive indicator that does not require media removal & utilizes same…

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Enhance your calcium screens with FLIPR Calcium 6 Assay Kits

Application Note

Enhance your calcium screens with FLIPR Calcium 6 Assay Kits

FLIPR Calcium Assay Kits from Molecular Devices employ sensitive calcium indicators and proprietary masking dyes to enable researchers to conduct highly sensitive…
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Calcium signaling with FLIPR Calcium 6 and 6-QF Assay Kits on the FlexStation 3 reader

Application Note

Calcium signaling with FLIPR Calcium 6 and 6-QF Assay Kits on the FlexStation 3 reader

FLIPR® Calcium Assay Kits from Molecular Devices employ sensitive calcium indicators and proprietary masking dyes to enable researchers to conduct highly sensitive…
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Data of FLIPR Calcium Assay Kits

  • FLIPR Calcium 6 Assay Kit signal window

    FLIPR Calcium 6 Assay Kit signal window

    Comparison of fluorescent signal response to calcium flux during carbachol agonism of Muscarinic M1 receptor in CHO M1 cells. This illustrates the signal-to-noise ratios offered by homogenous assay run in media using the FLIPR Calcium 6 Assay Kit. Combining a novel fluorophore and the proven quench technology, the FLIPR Calcium 6 Assay Kit shows the highest signal to background ratio.

  • Quencher free signal window

    When performing no-wash assays (initial media removal, replaced with assay buffer, no-wash after dye-loading) the novel fluorophore of FLIPR Calcium 6 Assay Kits demonstrates highest signal to background ratio. Obtain same high quality performance in presence or absence of masking dye.

    Quencher free signal window

  • Probenecid free signal window

    Probenecid free signal window

    Novel fluorophore is more resistant to organic anion exchange proteins found in some cell lines. No signal seen when using FLIPR Calcium 5 Assay Kit without probenecid (Ca5 Kit no PBX). With FLIPR Calcium 6 Assay Kit (Ca6), the signal is smaller without probenecid, however EC50s are conserved and Z' still greater than 0.5.

  • FLIPR Calcium 5, 4, & 3 Assay Kit comparison

    Comparison of fluorescent signal response to calcium flux during acetylcholine agonism of Muscarinic M1 receptor in CHO M1 cells. This illustrates the signal-to-noise ratios offered by various FLIPR Calcium Assay Kits with quench technology. Combining the novel fluorophore and the proven quench technology of the FLIPR Calcium 4 Assay Kit, the FLIPR Calcium 5 Assay Kit shows higher signal to background ratio than prior kits.

    FLIPR Calcium 4 & 3 Assay Kit comparison

  • Antagonism data with FLIPR Calcium 5 Assay Kit

    Antagonism data with FLIPR Calcium 5 Assay Kit

    Antagonism of calcium flux in response to EC80 acetylcholine in CHO M1 cells evaluated with the FLIPR Calcium 5 Assay Kit and the FLIPR Tetra System, demonstrating a large signal window and performance consistent with previously published data.

  • Signal trace comparison

    Signal trace on the FLIPR Tetra System of ATP agonism of PY receptor in HEK 293 cells compared using different calcium mobilization kits. Consistent EC50 values among all kits were recorded with no increase in background. The signal window was substantially larger for the FLIPR Calcium 5 Assay Kit.

    Signal trace comparison

  • FLIPR Calcium 4 & 3 Assay Kit comparison

    FLIPR Calcium 4 & 3 Assay Kit comparison

    Reduced background in the FLIPR Calcium 4 Assay Kit (orange) leads to improved ΔF/Fo values compared to the FLIPR Calcium 3 Assay Kit (green) in an assay with especially small calcium response. Assay: Endogenous response to carbachol in HEK 293 cells in a 384-well plate, 10,000 cells/well, media removal before assay on the FLIPR Tetra System.

  • FLIPR Calcium 4 and 3 Assay Kits vs competitor

    In many cases FLIPR Calcium 4 and Calcium 3 Assay Kits perform equally well while at the same time outperforming competitive kits as shown by a Molecular Devices customer in the data above. Assay: Undisclosed non-adherent cells stimulated with a protein activator, no media removal, assay was done on a FLIPR 3 instrument.

    FLIPR Calcium 4 and 3 Assay Kits vs competitor

Technology of FLIPR Calcium Assay Kits

  • FLIPR Calcium Assay Kits Provide a Homogeneous Solution for Assessing GPCRs and Ion Channels

    FLIPR Calcium Assay Kits Provide Homogeneous Solution for GPCRs and Ion Channels

    FLIPR Calcium Assay Kits utilize a calcium sensitive dye that is absorbed into the cell's cytoplasm during incubation. Upon ligand-receptor binding, intracellular calcium is released, binding with the dye, thereby increasing fluorescence intensity. All FLIPR Calcium Assay Kits utilize an extracellular masking technology to block background fluorescence and increase your assay's signal window. The FLIPR Calcium 6-QF Assay Kit is the one exception, enabling assay performance without a masking technology, making it an ideal choice for quench-sensitive applications such as multiplexing with luminescent indicators.

Ordering Options of FLIPR Calcium Assay Kits

FLIPR Calcium 6 Assay Kit

Evaluation Kit (1 Plate) - PN: R8194

  • (3) vials of component A* for Calcium 6
  • (3) vials of component A for Calcium 6-QF
  • (1) bottle of dilution buffer (Component B)
  • (3) vials of component C

* Each reagent vial (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 3 Calcium 6 assay plates and 3 Calcium 6-QF assay plates.

#R8194

$510.00 USD

1
Add to cart

Explorer Kit (10 Plates) - PN: R8190

  • (10) vials of component A*
  • (1) bottle of dilution buffer (Component B)

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

#R8190

$904.00 USD

1
Add to cart

Bulk Kit (100 Plates) - PN: R8191

  • Component A (10 vials*) 

* Each vial is sufficient for 10 plates (96- and 384-well), or 7 plates (1536-well).

The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well).

#R8191

$4,692.00 USD

1
Add to cart

Express Kit (100 Plates) - PN: R8195

  • Component A (2 vials*)

* Each vial is sufficient for 50 plates (96- and 384-well), or 35 plates (1536-well).

The entire Express Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well).

#R8195

$4,692.00 USD

1
Add to cart

FLIPR Calcium 6-QF Assay Kit

Explorer Kit - PN: R8192

  • (10) vials of component A*
  • (1) bottle of dilution buffer (Component B)
  • (10) vials of component C

* Each reagent vial (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

#R8192

$778.00 USD

1
Add to cart

Bulk Kit - PN: R8193

  • (10) vials of component A*
  • (10) vials of component C

* Each reagent vial (Component A, C) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8193

$4,390.00 USD

1
Add to cart

Express Kit - PN: R8196

  • (2) vials of component A*
  • (2) vials of component C

* Each reagent vial (Component A, C) is sufficient for 50 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8196

$4,390.00 USD

1
Add to cart

FLIPR Calcium 5 Assay Kit

Explorer Kit - PN: R8185

  • (10) vials of component A*
  • (1) bottle of dilution buffer (Component B)

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

#R8185

$708.00 USD

1
Add to cart

Bulk Kit - PN: R8186

  • (10) vials of component A*

* Each reagent vial (Component A) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8186

$4,332.00 USD

1
Add to cart

Express Kit - PN: R8187

  • (2) vials of component A*

* Each reagent vial (Component A) is sufficient for 50 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8187

$4,332.00 USD

1
Add to cart

FLIPR Calcium 4 Assay Kit

Explorer Kit - PN: R8142

  • (10) vials of component A*
  • (1) bottle of dilution buffer (Component B)

* Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). Each kit is sufficient for 10 plates.

#R8142

$928.00 USD

1
Add to cart

Bulk Kit - PN: R8141

  • (10) vials of component A*

* Each reagent vial (Component A) is sufficient for 10 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8141

$4,692.00 USD

1
Add to cart

Express Kit - PN: R8143

  • (2) vials of component A*

* Each reagent vial (Component A) is sufficient for 50 plates (96-, 384-, 1536-well). Each kit is sufficient for 100 plates.

#R8143

$4,402.00 USD

1
Add to cart

Resources of FLIPR Calcium Assay Kits

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Presentations
Videos & Webinars
Optogenetics and the FLIPR: How to Use Light to Set up a Cav1.3 High-Throughput Screening Assay

Optogenetics and the FLIPR: How to Use Light to Set up a Cav1.3 High-Throughput Screening Assay

How can we help advance your next big discovery?

Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?

I’d like to…

Request a demo

How can we help advance your next big discovery?

Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?

I’d like to…

Request a demo
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