FLIPR Calcium Assay Kit
Built as the most comprehensive portfolio of calcium reagents, the kits deliver pre-optimized, homogeneous, fluorescence-based formulations to expedite assay development and screening of GPCR and ion channel targets. FLIPR Calcium Assay Kits provide the most comprehensive method for detecting intracellular calcium changes in a simple and reliable homogeneous assay format. Select from six formulations to complement your target of interest.
FLIPR Assay Kits employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits.
Key Assay Features and Benefits
- Universal mix-and-read protocol accelerates assay workflow and increases throughput
- Superior signal-to-noise ratio facilitates confirmation of endogenous or transiently transfected receptor activity during assay development
- Pre-optimized and validated protocols for the FLIPR® Tetra and FlexStation® Systems ensure you can navigate both routine and unconventional cell lines and targets
FLIPR Calcium 6 and 6-QF Assay Kits
FLIPR Calcium 5 Assay Kit
FLIPR Calcium 4 Assay Kit
FLIPR Calcium 3 Assay Kit
Technology of FLIPR Calcium Assay Kits
FLIPR Calcium Assay Kits Provide a Homogeneous Solution for Assessing GPCRs and Ion Channels
FLIPR Calcium Assay Kits utilize a calcium sensitive dye that is absorbed into the cell's cytoplasm during incubation. Upon ligand-receptor binding, intracellular calcium is released, binding with the dye, thereby increasing fluorescence intensity. All FLIPR Calcium Assay Kits utilize an extracellular masking technology to block background fluorescence and increase your assay's signal window. The FLIPR Calcium 6-QF Assay Kit is the one exception, enabling assay performance without a masking technology, making it an ideal choice for quench-sensitive applications such as multiplexing with luminescent indicators.
Resources of FLIPR Calcium Assay Kits
FLIPR Calcium Assay Kits from Molecular Devices employ sensitive calcium indicators and proprietary masking dyes to enable researchers to conduct…
Gq protein-coupled receptor activation is commonly monitored in live cells in real time using calcium-sensitive dyes on a fluorescence plate reader.…
FLIPR® Calcium Assay Kits from Molecular Devices employ sensitive calcium indicators and proprietary masking dyes to enable researchers to conduct…
Combining a novel fluorophore and proven masking technology, the FLIPR Calcium 5 Assay Kit delivers reliable pharmacology, a larger signal window,…
Eliminate cardiotoxic compounds and identify potential cardiac drug candidates earlier in the drug discovery process with the FLIPR Tetra System,…
The FLIPR Tetra System delivers a screening platform to address real-time kinetic cell-based assays in 96-, 384- and 1536-well format. Regardless of…
In this study, we demonstrate the novel utility of optogenetic tools to control membrane potential in a reversible and precise fashion for screening…
This PDF describes optimization of the TRP (transient receptor potential) channel assay on the FLIPR TETRA instrument using hTRPV1-HEK293 cells and…
This poster compares the performance of cryopreserved "frozen" cells vs. cells in culture when used for screening GPRC drug candidates on the FLIPR…
This poster explores FLIPR Calcium 5 Assay Kit containing a superior performance calcium-sensitive indicator that does not require media removal…
Explore this scientific poster see how a novel calcium-sensing dye (FLIPR Calcium 6 Assay Kit) was used for Ligand Gated Ion channels (LIGCs).
Get detailed information about Calcium release-activated calcium channels by exploring this PDF. Learn how CRAC channels are playing an important r…
Cell based assays on FLIPR Tetra System to compare a novel FLIPRCcalcium 6 and 6-QF assay to other fluorescence-based calcium flux assays.
Optogenetics and the FLIPR: How to Use Light to Set up a Cav1.3 High-Throughput Screening Assay
Compatible Products & Services of FLIPR Calcium Assay Kits
Ready to get started?
We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.