FLIPR Calcium Assay Kit
Built as the most comprehensive portfolio of calcium reagents, the kits deliver pre-optimized, homogeneous, fluorescence-based formulations to expedite assay development and screening of GPCR and ion channel targets. FLIPR Calcium Assay Kits provide the most comprehensive method for detecting intracellular calcium changes in a simple and reliable homogeneous assay format. Select from six formulations to complement your target of interest.
FLIPR Assay Kits employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits.
Key Assay Features and Benefits
- Universal mix-and-read protocol accelerates assay workflow and increases throughput
- Superior signal-to-noise ratio facilitates confirmation of endogenous or transiently transfected receptor activity during assay development
- Pre-optimized and validated protocols for the FLIPR® Tetra and FlexStation® Systems ensure you can navigate both routine and unconventional cell lines and targets
FLIPR Calcium 6 and 6-QF Assay Kits
FLIPR Calcium 5 Assay Kit
FLIPR Calcium 4 Assay Kit
FLIPR Calcium 3 Assay Kit
Data of FLIPR Calcium Assay Kits
FLIPR Calcium 6 Assay Kit signal window
Comparison of fluorescent signal response to calcium flux during carbachol agonism of Muscarinic M1 receptor in CHO M1 cells. This illustrates the signal-to-noise ratios offered by homogenous assay run in media using the FLIPR Calcium 6 Assay Kit. Combining a novel fluorophore and the proven quench technology, the FLIPR Calcium 6 Assay Kit shows the highest signal to background ratio.
Quencher free signal window
When performing no-wash assays (initial media removal, replaced with assay buffer, no-wash after dye-loading) the novel fluorophore of FLIPR Calcium 6 Assay Kits demonstrates highest signal to background ratio. Obtain same high quality performance in presence or absence of masking dye.
Probenecid free signal window
Novel fluorophore is more resistant to organic anion exchange proteins found in some cell lines. No signal seen when using FLIPR Calcium 5 Assay Kit without probenecid (Ca5 Kit no PBX). With FLIPR Calcium 6 Assay Kit (Ca6), the signal is smaller without probenecid, however EC50s are conserved and Z' still greater than 0.5.
FLIPR Calcium 5, 4, & 3 Assay Kit comparison
Comparison of fluorescent signal response to calcium flux during acetylcholine agonism of Muscarinic M1 receptor in CHO M1 cells. This illustrates the signal-to-noise ratios offered by various FLIPR Calcium Assay Kits with quench technology. Combining the novel fluorophore and the proven quench technology of the FLIPR Calcium 4 Assay Kit, the FLIPR Calcium 5 Assay Kit shows higher signal to background ratio than prior kits.
Antagonism data with FLIPR Calcium 5 Assay Kit
Antagonism of calcium flux in response to EC80 acetylcholine in CHO M1 cells evaluated with the FLIPR Calcium 5 Assay Kit and the FLIPR Tetra System, demonstrating a large signal window and performance consistent with previously published data.
Signal trace comparison
Signal trace on the FLIPR Tetra System of ATP agonism of PY receptor in HEK 293 cells compared using different calcium mobilization kits. Consistent EC50 values among all kits were recorded with no increase in background. The signal window was substantially larger for the FLIPR Calcium 5 Assay Kit.
FLIPR Calcium 4 & 3 Assay Kit comparison
Reduced background in the FLIPR Calcium 4 Assay Kit (orange) leads to improved ΔF/Fo values compared to the FLIPR Calcium 3 Assay Kit (green) in an assay with especially small calcium response. Assay: Endogenous response to carbachol in HEK 293 cells in a 384-well plate, 10,000 cells/well, media removal before assay on the FLIPR Tetra System.
FLIPR Calcium 4 and 3 Assay Kits vs competitor
In many cases FLIPR Calcium 4 and Calcium 3 Assay Kits perform equally well while at the same time outperforming competitive kits as shown by a Molecular Devices customer in the data above. Assay: Undisclosed non-adherent cells stimulated with a protein activator, no media removal, assay was done on a FLIPR 3 instrument.
Technology of FLIPR Calcium Assay Kits
FLIPR Calcium Assay Kits Provide a Homogeneous Solution for Assessing GPCRs and Ion Channels
FLIPR Calcium Assay Kits utilize a calcium sensitive dye that is absorbed into the cell's cytoplasm during incubation. Upon ligand-receptor binding, intracellular calcium is released, binding with the dye, thereby increasing fluorescence intensity. All FLIPR Calcium Assay Kits utilize an extracellular masking technology to block background fluorescence and increase your assay's signal window. The FLIPR Calcium 6-QF Assay Kit is the one exception, enabling assay performance without a masking technology, making it an ideal choice for quench-sensitive applications such as multiplexing with luminescent indicators.
Resources of FLIPR Calcium Assay Kits
FLIPR Calcium Assay Kits from Molecular Devices employ sensitive calcium indicators and proprietary masking dyes to enable researchers to conduct highly sensitive fluorescent screens…
Gq protein-coupled receptor activation is commonly monitored in live cells in real time using calcium-sensitive dyes on a fluorescence plate reader. Automated liquid handling within…
FLIPR® Calcium Assay Kits from Molecular Devices employ sensitive calcium indicators and proprietary masking dyes to enable researchers to conduct highly sensitive fluorescent screens…
Combining a novel fluorophore and proven masking technology, the FLIPR Calcium 5 Assay Kit delivers reliable pharmacology, a larger signal window, and improved assay performance. With…
Eliminate cardiotoxic compounds and identify potential cardiac drug candidates earlier in the drug discovery process with the FLIPR Tetra System, together with ScreenWorks Peak Pro…
The FLIPR Tetra System delivers a screening platform to address real-time kinetic cell-based assays in 96-, 384- and 1536-well format. Regardless of the FLIPR Tetra System microplate…
In this study, we demonstrate the novel utility of optogenetic tools to control membrane potential in a reversible and precise fashion for screening state-dependent calcium channel…
Validation of TRPV1 and ASIC1 Ligand-Gated Ion Channels using Automated Patch Clamp and FLIPR with novel Ca2+ detection dye
In one series of experiments, we constructed and validated cell based assays in which the FLIPR Calcium 6 assay kit containing this novel calcium fluorophore was used to examine the act…
Optimizing a Transient Receptor Potential (TRP) Channel Assay on the FLIPRTETRA® Instrument using hTRPV1-HEK293 Cells and the FLIPR® Calcium 5 Assay Kit
This PDF describes optimization of the TRP (transient receptor potential) channel assay on the FLIPR TETRA instrument using hTRPV1-HEK293 cells and the FLIPR calcium 5 assay kit.
Functional Characterisation of Recombinant Muscarinic M3 Receptors in Assay-Ready Frozen Cells using a Novel Calcium Assay Kit on the FLIPRTETRA® Instrument
This poster compares the performance of cryopreserved "frozen" cells vs. cells in culture when used for screening GPRC drug candidates on the FLIPR Tetra instrument.
Cell Based Assays on the FLIPR TETRA® System : Comparison of the FLIPR Calcium 5 Assay Kit to Other Fluorescence Based Calcium Flux Assays
This poster explores FLIPR Calcium 5 Assay Kit containing a superior performance calcium-sensitive indicator that does not require media removal & utilizes same proven quench techno…
Calcium Release-Activated Channel (CRAC) Assays on the FLIPR® Tetra System: Evaluation of a Novel Fluorescent Calcium Reagent
Get detailed information about Calcium release-activated calcium channels by exploring this PDF. Learn how CRAC channels are playing an important role in intracellular Ca2+ homeostasis.
Cell Based Assays on the FLIPR® Tetra System: Comparison of a Novel FLIPR® Calcium Assay to other Fluorescence based Calcium Flux Assays
Cell based assays on FLIPR Tetra System to compare a novel FLIPRCcalcium 6 and 6-QF assay to other fluorescence-based calcium flux assays.
Videos and Demos
Optogenetics and the FLIPR: How to Use Light to Set up a Cav1.3 High-Throughput Screening Assay
Compatible Products & Services of FLIPR Calcium Assay Kits
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