COVID-19 RESPONSE - We are committed to supporting our scientific community during this pandemic. Learn more 

Easy-to-use software enabling robust and high-throughput cardiotoxicity screening using the FLIPR Penta system

 

Bring simplicity to your multi-user environment with unique protocols utilizing ScreenWorks® Software on the FLIPR® Penta High-Throughput Cellular Screening System. The software controls equipment intelligence and instrument motion as well as capturing data for analysis and export during highly kinetic assays. It records the signal from both standard GPCR assays in fluorescent or luminescent format and up to 100 fluorescent images per second during an iPSC cardiomyocyte fast calcium oscillation assay. The optional add-on, Peak Pro 2 software module, enables you to perform advanced peak analysis. It is especially helpful for analysis of iPSC derived cardiomyocyte and neuron calcium oscillation assays.

  • Bring simplicity to a multi-user environment

    Bring simplicity to a multi-user environment

    Protocol setup is flexible and user friendly – only relevant options are displayed, based on the installed fluidics and optics hardware, making it easy to choose parameters that are right for each assay.

  • Easily capture data for analysis and export

    Easily capture data for analysis and export

    Live and information-rich data in 96-, 384-, or 1536-well plate format is displayed during testing. Specific wells can be grouped for analysis or data export purposes.

  • Intuitive, user-friendly software interface

    Intuitive, user-friendly software interface

    Graphs and tables can be easily copied and pasted into documents, presentations or worksheets, and different reductions can be selected for group statistics, making results readily accessible.

ScreenWorks Software after a basic GPCR calcium flux protocol has been executed. In analysis mode, data grouping and a four parameter curve fit make it possible to quickly calculate the EC50 value from the curve.

ScreenWorks Software after a basic GPCR calcium flux protocol has been executed. In analysis mode, data grouping and a four parameter curve fit make it possible to quickly calculate the EC50 value from the curve.

Features

  • Fluorescent or luminescent read modes

    Fluorescent or luminescent read modes

    Standard fluorescence or optional aequorin luminescence detection read modes

  • Reduced screening time

    Reduced screening time

    Simultaneous transfer of 96, 384, or 1536 liquid or cells in suspension

  • Versatility

    Versatility

    Complex quadrant, multiple aspiration, or dispense liquid handling

  • Kinetic reduction

    Kinetic reduction

    Single or ratiometric kinetic cell-based reading

  • Automated protocols

    Automated protocols

    Simple-to-create automation protocols with cell suspension delivery

  • Optional Peak Pro 2 software module

    Optional Peak Pro 2 software module

    Added module with easy signal oscillation anomaly detection

High-throughput early toxicity screening with optional Peak Pro 2 software module

 

Advanced peak detection and event characterization measurements and analysis functionality are available with the optional Peak Pro 2 software module. This data analysis capability is especially useful with cardiomyocyte and neuronal protocols. The Peak Pro 2 analysis algorithm reduces false detections without the need to condition the raw data using search vectors.

The FLIPR Penta system with high-speed camera (HS EMCCD) option, for monitoring calcium oscillation in response to GPCR and ion channel modulator with Peak Pro 2 software module, allows you to measure and analyze patterns of calcium oscillations before and after treatment of human iPSC-derived cardiomyocytes and neurons. Images can be taken up to 100 times per second. You can analyze patterns using more than 30 parameters.

New safety measures in the drug discovery world, such as the NIH CiPA initiative, have placed added emphasis on early assessment of clinical safety and potential. The instrument hardware flexibility and easy-to-use Peak Pro 2 software module can help you assess and quantitate oscillation patterns consistent with potential cardiotoxicity earlier in the drug discovery process. It is also possible to characterize Ca2+ oscillation patterns in neuronal cells and spheroids. Panel A shows some of the parameters that you can measure. Panel B shows control and two examples of oscillation patterns.

ScreenWorks Peak Pro 2 software module

ScreenWorks Peak Pro 2 software module

Panel A) Calcium oscillation measurements available in Peak Pro 2 software module. Panel B) Calcium oscillations: Control; E-4031: hERG blocker; Dofetilide: oscillation irregularities.

 


 

ScreenWorks Software is part of the FLIPR Penta High-Throughput Cellular Screening System

High-throughput kinetic screening for toxicology and lead compound identification

  • High speed, high sensitivity EMCCD camera for both fluorescent and luminescent detection
  • Data acquisition of up to 100 Hz
  • Peak Pro 2 analysis module with easy oscillation anomaly detection
  • Over 30 peak measurement options
FLIPR Penta High-Throughput Cellular Screening System

FLIPR Penta High-Throughput Cellular Screening System

 

Applications of ScreenWorks Software

  • Calcium Flux

    Calcium Flux

    The evaluation of calcium flux is a long accepted, tried and true measure of cellular activity. Calcium flux can be used as a measurement for a host of cellular processes including neurotransmitter release, GPCR activity, voltage or ligand gated ion channels, and cardiomyocyte beat patterns, among many others. The FLIPR Penta System is the drug discovery tool for evaluation of calcium flux in high and ultra-high throughput due to its ease of use, sensitivity and user configurability.

    Cardiomyocyte screening

    Cardiomyocyte Screening

    Early prediction of drug-induced functional cardiotoxicity requires robust in vitro systems suitable for high-throughput screening. Readily-available iPSC-derived human cardiomyocytes may be used in conjunction with calcium-sensitive dyes, and beat rates are monitored as changes in intracellular calcium. These calcium peaks can be analyzed with our ScreenWorks PeakPro software which provides the investigator a suite of powerful tools for quantification of cardiomyocyte activity.

  • GPCR

    GPCR App

    Fluorescent dyes sensitive to changes in intracellular calcium have become increasingly popular in G protein-coupled receptor (GPCR) drug discovery. These dyes make GPCR screening assays highly sensitive, easy to perform, and low cost compared to other assays. Instruments capable of measuring fluorescent-based calcium indicators in high-throughput have become significantly more affordable making them obtainable even for academic groups.

    Read Application Note  

    Membrane Potential

    Membrane Potential

    There are dozens of identified disorders caused by impaired ion channel function. Ion channels are important drug discovery targets because they are highly druggable and specific. The FLIPR® Membrane Potential Assay Kits provide a fast, simple and reliable fluorescence-based assay for detecting changes in voltage across the cell membrane mediated by ion channel to evaluate target or off-target effects based on the application.

    Read Application Note  

  • Neuronal assays

    Neuronal Assays

    The FLIPR Penta System is an excellent tool for monitoring calcium flux related to neuronal activity. Newly developed commercially available iPSC neurons have made high-throughput neuronal screening a reality. These cells and assays are essential for monitoring a variety of factors including neural cell culture conditions, drug compound effects, and the effect of environmental neurotoxins. Neuronal activity and neurotransmitter release can be evaluated with fluorescence-based calcium indicator dyes combining fast, simple, and powerful assays with scalable throughput.

    Potassium Assays

    Potassium assays

    The majority of ion channel drug discovery solutions focus on potassium channels since they are the most prevalent type of ion channel, functioning in cardiac muscle action and hormone release. Potassium assay kits can be used to measure the effect of drug molecules on thallium transport through the potassium channel as a surrogate for potassium channel activity.

    Potassium assay resources:

Resources of ScreenWorks Software

Presentations
Videos & Webinars
CiPA Initiative and Validation

CiPA initiative and validation of high-throughput methods to characterize compound effects in human iPSC-derived cardiomyocytes on the FLIPR Penta system

ScreenWorks Software

Ordering information Part Number
ScreenWorks Software Site License® 0320-0392
ScreenWorks Peak Pro 1 Software module for Screenworks 5.x Software 5075630
ScreenWorks Peak Pro 2 Software module 5074843
ScreenWorks Peak Pro 1 Software module for Screenworks 4.2 Software and lower 5016900

*The host pc that ships with the Flipr Penta system is preinstalled with Screenworks 5.x Software. One license of Screenworks 5.x Software supports five installations, including one for the host PC controlling the Flipr Penta system, and four for data analysis PCs.

**Peak Pro 2 software module is only compatible with Screenworks 5.x software and above. A 14-day trial is included with Screenworks Software.