Easy-to-use software enabling robust and high-throughput cardiotoxicity screening using the FLIPR Penta system
Bring simplicity to your multi-user environment with unique protocols utilizing ScreenWorks® Software on the FLIPR® Penta High-Throughput Cellular Screening System. The software controls equipment intelligence and instrument motion as well as capturing data for analysis and export during highly kinetic assays. It records the signal from both standard GPCR assays in fluorescent or luminescent format and up to 100 fluorescent images per second during an iPSC cardiomyocyte fast calcium oscillation assay. The optional add-on, Peak Pro 2 software module, enables you to perform advanced peak analysis. It is especially helpful for analysis of iPSC derived cardiomyocyte and neuron calcium oscillation assays.
Bring simplicity to a multi-user environment
Protocol setup is flexible and user friendly – only relevant options are displayed, based on the installed fluidics and optics hardware, making it easy to choose parameters that are right for each assay.
Easily capture data for analysis and export
Live and information-rich data in 96-, 384-, or 1536-well plate format is displayed during testing. Specific wells can be grouped for analysis or data export purposes.
Intuitive, user-friendly software interface
Graphs and tables can be easily copied and pasted into documents, presentations or worksheets, and different reductions can be selected for group statistics, making results readily accessible.
ScreenWorks Software after a basic GPCR calcium flux protocol has been executed. In analysis mode, data grouping and a four parameter curve fit make it possible to quickly calculate the EC50 value from the curve.
Fluorescent or luminescent read modes
Standard fluorescence or optional aequorin luminescence detection read modes
Reduced screening time
Simultaneous transfer of 96, 384, or 1536 liquid or cells in suspension
Complex quadrant, multiple aspiration, or dispense liquid handling
Single or ratiometric kinetic cell-based reading
Simple-to-create automation protocols with cell suspension delivery
Optional Peak Pro 2 software module
Added module with easy signal oscillation anomaly detection
High-throughput early toxicity screening with optional Peak Pro 2 software module
Advanced peak detection and event characterization measurements and analysis functionality are available with the optional Peak Pro 2 software module. This data analysis capability is especially useful with cardiomyocyte and neuronal protocols. The Peak Pro 2 analysis algorithm reduces false detections without the need to condition the raw data using search vectors.
The FLIPR Penta system with high-speed camera (HS EMCCD) option, for monitoring calcium oscillation in response to GPCR and ion channel modulator with Peak Pro 2 software module, allows you to measure and analyze patterns of calcium oscillations before and after treatment of human iPSC-derived cardiomyocytes and neurons. Images can be taken up to 100 times per second. You can analyze patterns using more than 30 parameters.
New safety measures in the drug discovery world, such as the NIH CiPA initiative, have placed added emphasis on early assessment of clinical safety and potential. The instrument hardware flexibility and easy-to-use Peak Pro 2 software module can help you assess and quantitate oscillation patterns consistent with potential cardiotoxicity earlier in the drug discovery process. It is also possible to characterize Ca2+ oscillation patterns in neuronal cells and spheroids. Panel A shows some of the parameters that you can measure. Panel B shows control and two examples of oscillation patterns.
Panel A) Calcium oscillation measurements available in Peak Pro 2 software module. Panel B) Calcium oscillations: Control; E-4031: hERG blocker; Dofetilide: oscillation irregularities.
ScreenWorks Software is part of the FLIPR Penta High-Throughput Cellular Screening System
High-throughput kinetic screening for toxicology and lead compound identification
- High speed, high sensitivity EMCCD camera for both fluorescent and luminescent detection
- Data acquisition of up to 100 Hz
- Peak Pro 2 analysis module with easy oscillation anomaly detection
- Over 30 peak measurement options
FLIPR Penta High-Throughput Cellular Screening System
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Applications of ScreenWorks Software
Resources of ScreenWorks Software
*The host pc that ships with the Flipr Penta system is preinstalled with Screenworks 5.x Software. One license of Screenworks 5.x Software supports five installations, including one for the host PC controlling the Flipr Penta system, and four for data analysis PCs.
**Peak Pro 2 software module is only compatible with Screenworks 5.x software and above. A 14-day trial is included with Screenworks Software.