Patch clamp electrophysiology
Electrophysiology is one of the foundational disciplines in neuroscience and cardiac physiology for the evaluation of ion channels. The Patch-clamp technique is a versatile electrophysiological tool for understanding ion channel behavior.
Every cell expresses ion channels, but the most common cells to study with patch-clamp techniques include neurons, muscle fibers, cardiomyocytes, and oocytes overexpressing single ion channels. To evaluate single ion channel conductance, a microelectrode forms a high resistance seal with the cellular membrane, and a patch of cell membrane containing the ion channel of interest is removed. Alternatively, while the microelectrode is sealed to the cell membrane, this small patch can be ruptured giving the electrode electrical access to the whole cell. Voltage is then applied, forming a voltage clamp, and membrane current is measured. Current clamp can also be used to measure changes in membrane voltage called membrane potential. Voltage or current change within cell membranes can be altered by applying compounds to block or open channels. These techniques enable researchers to understand how ion channels behave both in normal and disease states and how different drugs, ions, or other analytes can modify these conditions.
Resources of Electrophysiology
Videos and Demos
How to Combine Traces, Calculate Rise or Decay Time Constant, and Perform Curve Fitting Using Axon pCLAMP Software
How to Create Customized Command Waveforms Using the pCLAMP Software
The Use of Sequencing Keys, User List, and Stimulus File with pCLAMP Software
Synchronizing Electrophysiology and Imaging Solution with Axon pCLAMP and MetaMorph Software
Online Statistics, Membrane Test Between Sweeps in Clampex and Analysis of Synaptic Events with the Clampfit™ Data Analysis
Use of the Axoporator 800A for Single-cell Electroporation for Transfection and Dye-labeling
Using the Axoclamp 900A for Two-Electrode Voltage-Clamp of Xenopus Oocytes Expressing Ion Channels
Writing Long-Term Potentiation and Depression Protocols and the Use of Filters in Data Acquisition and the Clampfit Application
Series Resistance Compensated or Not
Using Electrophysiological Studies to Accelerate Mechanistic Study in Reception and Transmission
Update and Hardware Choices for Optogenetics Considerations for Synchronized Light Patterning
Investigations of the Effects of Amyloid-Beta Proteins on hSlo1.1, a BK Channel, in a Xenopus Oocyte Model
Nanopores-Electronic Tools for Single-Molecule Biophysics and Bio-Nanotechnologies
Axon Amplifiers and pCLAMP Software-key Features Reviews (Chinese version)
Basic Single Channel Analysis Using Clampfit
Action Potential Analysis in Clampfit Module
A Walkthrough of the Protocol Editor in the pCLAMP Data Acquisition Module
A Walkthrough of Protocol Editor in pCLAMP (Chinese version)
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