EarlyTox Cardiotoxicity Kit
The EarlyTox™ Cardiotoxicity Kit provides a fast, simple, and reliable fluorescence-based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes. Leveraging our proprietary masking technology with our novel calcium sensitive indicator, researchers can:
- Eliminate cardiotoxic compounds and identify potential drug candidates
- Measure 384 samples in minutes rather than hours
- Minimize non-specific effect of dye on beat characteristics
- Reduce well-to-well variation and improve data quality
- Largest signal dynamic range available
- Benchmark against three reference compounds: isoproterenol, sotalol and propranolol
Latest Resources
Data of EarlyTox Cardiotoxicity Kit
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Comparison of reference compounds on cardiomyocyte beat rate
(A) Calcium signal change in untreated iPSC cardiomyocytes incubated for two hours with EarlyTox Cardiotoxicity Dye indicated by calcium peaks. (B) Propranolol slows peak frequency of calcium in iPSC cells. (C) Isoproterenol increases calcium peak frequency in iPSC cardiomyocytes. (D) Sotalol slows peak frequency of calcium in iPSC cardiomyocytes and alters the normal pattern.
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Beat frequency comparison
Beat frequencies were recorded during a four hour period. EarlyTox Cardiotoxicity Dye had the highest beat frequency in all wells.
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Impact of dye on averge peak amplitude
Over time, EarlyTox Cardiotoxicity Dye had the highest average peak amplitude. Signal from the other dyes decreased over time.
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Comparison of impact of dye on cariomyocyte beating over time
Thirty-four control wells were compared with each dye over a four hour period. All of the EarlyTox Cardiotoxicity Dye wells remained beating up to four hours. Silenced wells occurred much earlier in the Fluo-4 Direct wells and the FLIPR Calcium 5 Kit wells and were nearly all gone by four hours.
Technology of EarlyTox Cardiotoxicity Kit
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EarlyTox™ Cardiotoxicity Kits provide a homogeneous solution to predict compound toxicity and efficacy
EarlyTox Cardiotoxicity Kits utilize a calcium sensitive dye that is absorbed into the cell's cytoplasm during incubation. When dye binds with calcium ions in the cell cytoplasm, fluorescent intensity increases, enabling measurement of any changes in calcium concentration. The masking technology does not enter the cell, but reduces extracellular background fluorescence. This helps improve the assay signal window, providing more detail to the beat patterns.
Role of calcium in the mechanism of cardiomyocyte contraction-relaxation
EarlyTox Cardiotoxicity Kit can be used for characterizing the impact of pharmacological compounds on peak frequency (BPM), peak amplitude and beat pattern and is designed to work with stem cell-derived cardiomyocytes or primary cardiomyocytes. Peak frequencies are determined from changes in intracellular calcium concentration monitored by the EarlyTox Cardiotoxicity Dye. Role of calcium in cardiomyocyte contraction-relaxation includes: 2.1. Membrane depolarization occurs, calcium channels open and calcium enters cytosol. 2.2. Intracellular calcium triggers calcium release from sarcoplasmic reticulum. 2.3. Cytoplasmic calcium binds to troponin, activates sarcomere. 2.4. Cardiomyocyte contraction occurs. 2.5. Removal of calcium by active transport into the sarcoplasmic reticulum and calcium exchange with extracellular fluid. 2.6. Cycle repeats.
Resources of EarlyTox Cardiotoxicity Kit
News
Molecular Devices and HeartBeat.bio announce collaboration to automate and scale cardiac organoids for high-throughput screening in drug discovery
Molecular Devices and HeartBeat.bio announce collaboration to automate and scale cardiac organoids for high-throughput screening in drug discovery
SAN JOSE, Calif., and VIENNA, Sept. 12, 2022 – Molecular Devices, LLC., a leading provider of high-performance life science solutions, and HeartBeat.bio AG, a biotech company…
Application Note
Calcium flux assay for in vitro neurotoxicity studies and drug screening
Calcium flux assay for in vitro neurotoxicity studies and drug screening
Calcium imaging, or monitoring changes in intracellular calcium, is an extremely useful technique for investigating the variety of roles that calcium ions have in functioning neurons.…
eBook
Optimized Assays for Breakthrough Research
Optimized Assays for Breakthrough Research
Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers.
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Application Note
Multiparametric assays for cardiomyocytes on a single platform
Multiparametric assays for cardiomyocytes on a single platform
Recently, it has been shown that changes in intracellular calcium flux associated with cardiomyocyte contractions can be monitored on a fluorescence imaging plate reader (FLIPR Tetra…
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Application Note
EarlyTox Cardiotoxicity Kit provides biologically relevant cardiotoxicity data earlier in the drug discovery process
EarlyTox Cardiotoxicity Kit provides biologically relevant cardiotoxicity data earlier in the drug discovery process
The EarlyTox Cardiotoxicity Kit contains a novel calcium sensitive dye and a proprietary masking technology optimized for measuring changes in cytoplasmic calcium which are associated…
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Application Note
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
The assessment of cardiotoxicity is important in the early stages of drug discovery to enable elimination of potentially toxic compounds from further development. This is critical to…
Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery
