EarlyTox Cardiotoxicity Kit

The EarlyTox™ Cardiotoxicity Kit provides a fast, simple, and reliable fluorescence-based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes. Leveraging our proprietary masking technology with our novel calcium sensitive indicator, researchers can:

  • Eliminate cardiotoxic compounds and identify potential drug candidates
  • Measure 384 samples in minutes rather than hours
  • Minimize non-specific effect of dye on beat characteristics
  • Reduce well-to-well variation and improve data quality
  • Largest signal dynamic range available
  • Benchmark against three reference compounds: isoproterenol, sotalol and propranolol

Technology of EarlyTox Cardiotoxicity Kit

  • EarlyTox Cardiotoxicity Kits Principle Diagram

    EarlyTox™ Cardiotoxicity Kits provide a homogeneous solution to predict compound toxicity and efficacy

    EarlyTox Cardiotoxicity Kits utilize a calcium sensitive dye that is absorbed into the cell's cytoplasm during incubation. When dye binds with calcium ions in the cell cytoplasm, fluorescent intensity increases, enabling measurement of any changes in calcium concentration. The masking technology does not enter the cell, but reduces extracellular background fluorescence. This helps improve the assay signal window, providing more detail to the beat patterns.

  • Role of Calcium in the Mechanism of Cardiomyocyte Contraction Relaxation

    Role of calcium in the mechanism of cardiomyocyte contraction-relaxation

    EarlyTox Cardiotoxicity Kit can be used for characterizing the impact of pharmacological compounds on peak frequency (BPM), peak amplitude and beat pattern and is designed to work with stem cell-derived cardiomyocytes or primary cardiomyocytes. Peak frequencies are determined from changes in intracellular calcium concentration monitored by the EarlyTox Cardiotoxicity Dye. Role of calcium in cardiomyocyte contraction-relaxation includes: 2.1. Membrane depolarization occurs, calcium channels open and calcium enters cytosol. 2.2. Intracellular calcium triggers calcium release from sarcoplasmic reticulum. 2.3. Cytoplasmic calcium binds to troponin, activates sarcomere. 2.4. Cardiomyocyte contraction occurs. 2.5. Removal of calcium by active transport into the sarcoplasmic reticulum and calcium exchange with extracellular fluid. 2.6. Cycle repeats.

Ordering Options of EarlyTox Cardiotoxicity Kit

EarlyTox Cardiotoxicity Kit

Explorer Kit

  • (2) Vials of Component A*
  • (1) Bottle of dilution buffer (Component B)
  • (1) Vial each of three reference compounds: isoproterenol, sotalol and propranolol

* Each reagent vial (Component A) is sufficient for 1 plate (96- or 384-wells). Each kit is sufficient for 2 plates.

#R8210

$330.00 USD

1

Bulk Kit

  • (2) Vials of Component A**
  • (1) Bottle of dilution buffer (Component B)
  • (1) Vial each of three reference compounds: isoproterenol, sotalol and propranolol

** Each reagent vial (Component A) is sufficient for 5 plates (96-, 384- or 1536-wells). Each kit is sufficient for 10 plates.

#R8211

$1,032.00 USD

1

Resources of EarlyTox Cardiotoxicity Kit

Videos and Demos

Vitro Assay Tools for Cardiac Toxicity and Discovery

Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery

Compatible Products & Services of EarlyTox Cardiotoxicity Kit

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.