EarlyTox Cardiotoxicity Kit

The EarlyTox™ Cardiotoxicity Kit provides a fast, simple, and reliable fluorescence-based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes. Leveraging our proprietary masking technology with our novel calcium sensitive indicator, researchers can:

  • Eliminate cardiotoxic compounds and identify potential drug candidates
  • Measure 384 samples in minutes rather than hours
  • Minimize non-specific effect of dye on beat characteristics
  • Reduce well-to-well variation and improve data quality
  • Largest signal dynamic range available
  • Benchmark against three reference compounds: isoproterenol, sotalol and propranolol

Data of EarlyTox Cardiotoxicity Kit

  • Comparison of reference compounds on cardiomyocyte beat rate

    Comparison of reference compounds on cardiomyocyte beat rate

    (A) Calcium signal change in untreated iPSC cardiomyocytes incubated for two hours with EarlyTox Cardiotoxicity Dye indicated by calcium peaks. (B) Propranolol slows peak frequency of calcium in iPSC cells. (C) Isoproterenol increases calcium peak frequency in iPSC cardiomyocytes. (D) Sotalol slows peak frequency of calcium in iPSC cardiomyocytes and alters the normal pattern.

  • Beat frequency comparison

    Beat frequencies were recorded during a four hour period. EarlyTox Cardiotoxicity Dye had the highest beat frequency in all wells.

    Beat frequency comparison

  • Impact of dye on averge peak amplitude

    Impact of dye on averge peak amplitude

    Over time, EarlyTox Cardiotoxicity Dye had the highest average peak amplitude. Signal from the other dyes decreased over time.

  • Comparison of impact of dye on cariomyocyte beating over time

    Thirty-four control wells were compared with each dye over a four hour period. All of the EarlyTox Cardiotoxicity Dye wells remained beating up to four hours. Silenced wells occurred much earlier in the Fluo-4 Direct wells and the FLIPR Calcium 5 Kit wells and were nearly all gone by four hours.

    Comparison of impact of dye on cariomyocyte beating over time

Technology of EarlyTox Cardiotoxicity Kit

  • EarlyTox Cardiotoxicity Kits Principle Diagram

    EarlyTox™ Cardiotoxicity Kits provide a homogeneous solution to predict compound toxicity and efficacy

    EarlyTox Cardiotoxicity Kits utilize a calcium sensitive dye that is absorbed into the cell's cytoplasm during incubation. When dye binds with calcium ions in the cell cytoplasm, fluorescent intensity increases, enabling measurement of any changes in calcium concentration. The masking technology does not enter the cell, but reduces extracellular background fluorescence. This helps improve the assay signal window, providing more detail to the beat patterns.

  • Role of Calcium in the Mechanism of Cardiomyocyte Contraction Relaxation

    Role of calcium in the mechanism of cardiomyocyte contraction-relaxation

    EarlyTox Cardiotoxicity Kit can be used for characterizing the impact of pharmacological compounds on peak frequency (BPM), peak amplitude and beat pattern and is designed to work with stem cell-derived cardiomyocytes or primary cardiomyocytes. Peak frequencies are determined from changes in intracellular calcium concentration monitored by the EarlyTox Cardiotoxicity Dye. Role of calcium in cardiomyocyte contraction-relaxation includes: 2.1. Membrane depolarization occurs, calcium channels open and calcium enters cytosol. 2.2. Intracellular calcium triggers calcium release from sarcoplasmic reticulum. 2.3. Cytoplasmic calcium binds to troponin, activates sarcomere. 2.4. Cardiomyocyte contraction occurs. 2.5. Removal of calcium by active transport into the sarcoplasmic reticulum and calcium exchange with extracellular fluid. 2.6. Cycle repeats.

Ordering Options of EarlyTox Cardiotoxicity Kit

EarlyTox Cardiotoxicity Kit

Explorer Kit

  • (2) Vials of Component A*
  • (1) Bottle of dilution buffer (Component B)
  • (1) Vial each of three reference compounds: isoproterenol, sotalol and propranolol

* Each reagent vial (Component A) is sufficient for 1 plate (96- or 384-wells). Each kit is sufficient for 2 plates.

#R8210

$330.00 USD

1

Bulk Kit

  • (2) Vials of Component A**
  • (1) Bottle of dilution buffer (Component B)
  • (1) Vial each of three reference compounds: isoproterenol, sotalol and propranolol

** Each reagent vial (Component A) is sufficient for 5 plates (96-, 384- or 1536-wells). Each kit is sufficient for 10 plates.

#R8211

$1,032.00 USD

1

Resources of EarlyTox Cardiotoxicity Kit

Videos and Demos

Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery

Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery

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We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.