EarlyTox Cardiotoxicity Kit
The EarlyTox™ Cardiotoxicity Kit provides a fast, simple, and reliable fluorescence-based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes. Leveraging our proprietary masking technology with our novel calcium sensitive indicator, researchers can:
- Eliminate cardiotoxic compounds and identify potential drug candidates
- Measure 384 samples in minutes rather than hours
- Minimize non-specific effect of dye on beat characteristics
- Reduce well-to-well variation and improve data quality
- Largest signal dynamic range available
- Benchmark against three reference compounds: isoproterenol, sotalol and propranolol
Technology of EarlyTox Cardiotoxicity Kit
EarlyTox™ Cardiotoxicity Kits provide a homogeneous solution to predict compound toxicity and efficacy
EarlyTox Cardiotoxicity Kits utilize a calcium sensitive dye that is absorbed into the cell's cytoplasm during incubation. When dye binds with calcium ions in the cell cytoplasm, fluorescent intensity increases, enabling measurement of any changes in calcium concentration. The masking technology does not enter the cell, but reduces extracellular background fluorescence. This helps improve the assay signal window, providing more detail to the beat patterns.
Role of calcium in the mechanism of cardiomyocyte contraction-relaxation
EarlyTox Cardiotoxicity Kit can be used for characterizing the impact of pharmacological compounds on peak frequency (BPM), peak amplitude and beat pattern and is designed to work with stem cell-derived cardiomyocytes or primary cardiomyocytes. Peak frequencies are determined from changes in intracellular calcium concentration monitored by the EarlyTox Cardiotoxicity Dye. Role of calcium in cardiomyocyte contraction-relaxation includes: 2.1. Membrane depolarization occurs, calcium channels open and calcium enters cytosol. 2.2. Intracellular calcium triggers calcium release from sarcoplasmic reticulum. 2.3. Cytoplasmic calcium binds to troponin, activates sarcomere. 2.4. Cardiomyocyte contraction occurs. 2.5. Removal of calcium by active transport into the sarcoplasmic reticulum and calcium exchange with extracellular fluid. 2.6. Cycle repeats.
Resources of EarlyTox Cardiotoxicity Kit
Cardiomyocyte beating and cytotoxicity assays on the SpectraMax i3 Multi-Mode Microplate Platform with SpectraMax MiniMax Imaging Cytometer
Recently, it has been shown that changes in intracellular calcium flux associated with cardiomyocyte contractions can be monitored on a fluorescence imaging plate reader (FLIPR Tetra…
EarlyTox Cardiotoxicity Kit provides biologically relevant cardiotoxicity data earlier in the drug discovery process
The EarlyTox Cardiotoxicity Kit contains a novel calcium sensitive dye and a proprietary masking technology optimized for measuring changes in cytoplasmic calcium which are associated…
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
The assessment of cardiotoxicity is important in the early stages of drug discovery to enable elimination of potentially toxic compounds from further development. This is critical to…
Videos and Demos
Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery
Compatible Products & Services of EarlyTox Cardiotoxicity Kit
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