FLIPR Penta High-Throughput Cellular Screening System

High-throughput kinetic screening for toxicology and lead compound identification
An industry-renowned instrument for monitoring GPCRs and ion channels
Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per second and quickly cherry pick events such as Early Afterdepolarization-like events (EAD-like events), fibrillation, and irregular beating.
Since its introduction, the system has developed a reputation for reliability and ease of use. The system can be configured based on library size, detection mode, screening format, assay, and target, making the transition from assay development to lead optimization a seamless process.
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Assess toxicity effects
The HS-EMCCD camera option allows for up to 100 measurements per second, providing detailed information about cardiomyocyte and/or neuronal oscillation. Combined with the ScreenWorks® Peak Pro 2 software analysis module, compound-induced pro-arrhythmic effects such as EAD-like events can be easily identified and flagged.
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Identify lead compounds
With seven LED sets, many filter options, and fluorescence or luminescence detection, the FLIPR system supports many assays, including calcium flux, potassium, and membrane potential.
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Configure to your throughput
The FLIPR Penta system can be configured to match user needs. From manual assays to automated solutions, to measuring 96-, 384-, or 1536-well samples at a time, to using wash or no-wash assay kits in adherent or suspension cell mode, the system can be upgraded as needs change in the future.
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FLIPR Penta High-Throughput Cellular Screening System
Features
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Complex event analysis
ScreenWorks Peak Pro 2 software analysis, powered by pCLAMP 11, gives you the unique ability to characterize calcium oscillation peak data in one software program. Compounds can be flagged that cause waveform irregularities that may indicate hazards for arrhythmia, seizures, or other toxicity effects quickly and without having to export data. Distinctly different calcium oscillations in neurons can also be characterized.
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Configurable optics
The FLIPR Penta system can be matched to user needs starting with a standard EMCCD camera, a more cost-effective solution that is perfect for GPCR and Membrane Potential assays, or with the new HS EMCCD that can capture up to 100 images per second and offers exceptional luminescence performance. The camera, LEDs, and filters can be configured at the time of purchase or later when assay needs change.
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User-changeable pipette modules
Pipette heads can be changed in minutes without the use of tools from 96, 384, or 1536 channels to accommodate assay throughput needs.
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Ease of use
ScreenWorks software seamlessly incorporates drag-and-drop protocols, intuitive buttons, and color-coded plate layout to simplify workflows. View signal traces, EC50 curves, and Z factors on-the-fly without having to export data to another software program.
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Validated solution
Purchasing a FLIPR system from Molecular Devices assures users that they are investing in a technology that can grow with them. Hardware, software, and assay kits have been validated on the system by an industry-renowned leader with over 30 years of experience.
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Custom automation options*
The drug discovery process has shared methods, yet each project is unique. The Advanced Workflow Engineering Solutions Team offers a variety of customized services from automated compound plate delivery to fully integrated workstations with automated incubation.
*Price, time to deliver, and specifications will vary based on technical requirements agreed upon. Solution requirements may cause adjustment to standard performance.
ScreenWorks Software with customizable protocols and data analysis for high-throughput kinetic assays
The FLIPR Penta system utilizes our ScreenWorks Software to define and run experimental protocols. Using a drag-and-drop interface, protocols can be easily set up to include:
- Standard fluorescent or luminescent read modes or optional aequorin luminescence detection
- Simultaneous transfer of 96, 384, or 1536 liquid or cells in suspension
- Complex quadrant, multiple aspirations or multiple dispensing liquid handling
- Single or ratiometric kinetic cell-based reading
- Tip washing with up to two solvents
- Cell suspension delivery with automatable cleaning protocols
- Simple-to-create automation protocols
- Optional Peak Pro software module with easy signal oscillation anomaly detection
Latest Resources
Applications of FLIPR Penta High-Throughput Cellular Screening System
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Calcium Flux
The evaluation of calcium flux is a long accepted, tried and true measure of cellular activity. Calcium flux can be used as a measurement for a host of cellular processes including neurotransmitter release, GPCR activity, voltage or ligand gated ion channels, and cardiomyocyte beat patterns, among many others. The FLIPR Penta System is the drug discovery tool for evaluation of calcium flux in high and ultra-high throughput due to its ease of use, sensitivity and user configurability.
Cardiomyocyte screening
Early prediction of drug-induced functional cardiotoxicity requires robust in vitro systems suitable for high-throughput screening. Readily-available iPSC-derived human cardiomyocytes may be used in conjunction with calcium-sensitive dyes, and beat rates are monitored as changes in intracellular calcium. These calcium peaks can be analyzed with our ScreenWorks PeakPro software which provides the investigator a suite of powerful tools for quantification of cardiomyocyte activity.
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Cardiotoxicity
Cardiotoxicity or cardiac toxicity is a term used to define chemicals that are toxic to the heart, causing muscle damage or heart electrophysiology dysfunction.
The assessment of cardiotoxicity is important in the early stages of drug discovery to eliminate potentially toxic compounds from further development.
GPCRs (G protein-coupled receptors)
GPCRs or G protein-coupled receptors are proteins located on the cell surface that recognize extracellular substances and transmit signals across the cell membrane. GPCRs do this by activating guanine nucleotide-binding proteins (G protein) that are responsible for signal transduction inside the cell.
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Ion Channel
Ion channels are pores in the cellular membrane that allow the passage of ions across the impermeant lipid cell membrane. The flow of calcium, potassium, and sodium is very important in many cellular processes such as muscle contraction in the heart, pancreatic insulin release, the transmission of impulses in the nervous system such as pain, and T cell activation.
Membrane Potential
There are dozens of identified disorders caused by impaired ion channel function. Ion channels are important drug discovery targets because they are highly druggable and specific. The FLIPR® Membrane Potential Assay Kits provide a fast, simple and reliable fluorescence-based assay for detecting changes in voltage across the cell membrane mediated by ion channel to evaluate target or off-target effects based on the application.
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Neuronal assays
The FLIPR Penta System is an excellent tool for monitoring calcium flux related to neuronal activity. Newly developed commercially available iPSC neurons have made high-throughput neuronal screening a reality. These cells and assays are essential for monitoring a variety of factors including neural cell culture conditions, drug compound effects, and the effect of environmental neurotoxins. Neuronal activity and neurotransmitter release can be evaluated with fluorescence-based calcium indicator dyes combining fast, simple, and powerful assays with scalable throughput.
Potassium Assays
The majority of ion channel drug discovery solutions focus on potassium channels since they are the most prevalent type of ion channel, functioning in cardiac muscle action and hormone release. Potassium assay kits can be used to measure the effect of drug molecules on thallium transport through the potassium channel as a surrogate for potassium channel activity.
Potassium assay resources:
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Toxicology
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It is a growing concern in our world today as we are exposed to more and more chemicals, both in our environment and in the products we use.
Specifications & Options of FLIPR Penta High-Throughput Cellular Screening System
Resources of FLIPR Penta High-Throughput Cellular Screening System
Blog
Stem Cell Science and Regenerative Medicine – Technology and Methods Presented at ISSCR 2022
Stem Cell Science and Regenerative Medicine – Technology and Methods Presented at ISSCR 2022
It was another great year at ISSCR 2022! Leaders from across the globe came together to discuss new technologies, share insights, and explore the newest breakthroughs in stem cell…
Scientific Poster
Structural organization in 3D human iPSC-derived cardiac tri-culture microtissues
Structural organization in 3D human iPSC-derived cardiac tri-culture microtissues
The human heart is a complex organ providing highly regulated processes of moving blood through the body. The adult human ventricle is comprised of cardiomyocytes, endothelial cells,…
Blog
Stem cell science insights and breakthroughs presented at #ISSCR2021
Stem cell science insights and breakthroughs presented at #ISSCR2021
If you didn't get a chance to visit us at our poster sessions during ISSCR 2021, don’t fret. We've gathered all our sessions right here for you. The ISSCR Annual Meeting brought…
Brochure
ScreenWorks Software
ScreenWorks Software
Bring simplicity to your multi-user environment with unique protocols utilizing ScreenWorks® Software on the FLIPR® Penta High-Throughput Cellular Screening System. The software cont…
Scientific Poster
High-throughput assessment of compound-induced pro-arrhythmic effects in human IPSC-derived cardiomyocytes
High-throughput assessment of compound-induced pro-arrhythmic effects in human IPSC-derived cardiomyocytes
Development of biologically relevant and predictive cell-based assays for compound screening and toxicity assessment is a major challenge in drug discovery. The focus of this study w…
Data Sheet
Pin tool liquid handling for the FLIPR system
Pin tool liquid handling for the FLIPR system
The FLIPR® High-Throughput Cellular Screening System includes a pin tool option for nanoliter liquid transfer. The pin tool pipettor is a complementary addition to the existing 96-,…
Flyer
FLIPR® Penta and Tetra Systems
FLIPR® Penta and Tetra Systems
FLIPR® Penta and Tetra Systems Instrument LED and filter combinations.
eBook
Accelerate vaccine development
Accelerate vaccine development
Immunology is now, more than anytime in recent history, one of the top fields of research. Monoclonal antibodies (mAbs) continue to enjoy intense interest as potential therapeutics.…
Application Note
Development of optimized workflows to enable calcium mobilization assays on the SpectraMax iD3 and iD5 readers
Development of optimized workflows to enable calcium mobilization assays on the SpectraMax iD3 and iD5 readers
As a laboratory’s requirements often change according to ongoing research needs, having a multi-mode microplate reader that offers multiple detection modes such as absorbance,…
Publications
Advanced Cell Screening and Imaging Reveals How the Cardiac System Responds in Drug Development
Advanced Cell Screening and Imaging Reveals How the Cardiac System Responds in Drug Development
In drug discovery, time and monetary investments increase significantly the further candidates advance through the preclinical phase of testing, analysis and evaluation. By excluding…
Publications
Neuroscience: bridging the gap between cell-based and human research
Neuroscience: bridging the gap between cell-based and human research
Neurological disorders impact up to a billion people around the world and appear to be on the rise [1]. In fact, those affecting the central and peripheral nervous systems – includin…
Application Note
Phenotypic characterization of neuroactive compound effects
Phenotypic characterization of neuroactive compound effects
The FLIPR Penta system is powered by a high-speed EMCCD camera and the new ScreenWorks Peak Pro 2 software. The system allows measurement and analysis of complex patterns of calcium…
Scientific Poster
Multiplexed automated assays for neurotoxicity evaluation using induced pluripotent stem cell-derived neural 3D cell models
Multiplexed automated assays for neurotoxicity evaluation using induced pluripotent stem cell-derived neural 3D cell models
Cell-based phenotypic assays have become an increasingly attractive alternative to traditional in vitro and in vivo testing in pharmaceutical drug development and toxicological safet…
Scientific Poster
Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures
Complex event analysis for neurotoxic profiling of compound effects on human iPSC-derived neural spheroid 3D cultures
To speed up the development of more effective and safer drugs, there is an increasing need for more complex, biologically relevant and predictive cell-based assays for drug discovery…
Publications
Drug discovery & development award winners revealed at SLAS2020
Drug discovery & development award winners revealed at SLAS2020
The winners of the 2020 Scientists’ Choice Awards® were announced by SelectScience Editor-in-Chief Kerry Parker to an audience of industry leaders and scientists in San Di…
Application Note
Multi-parametric assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes
Multi-parametric assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes
Development of biologically relevant, predictive cell-based assays for compound screening and toxicity assessment is a major challenge in drug discovery.
Scientific Poster
Multi-parametric assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes
Multi-parametric assessment of compound-induced pro-arrhythmic effects in human iPSC-derived cardiomyocytes
Development of biologically relevant and predictive cell-based assays for compound screening and toxicity assessment is a major challenge in drug discovery
Brochure
FLIPR Penta High-Throughput Cellular Screening System
FLIPR Penta High-Throughput Cellular Screening System
Building on the success of the industry-leading FLIPR® platform for monitoring of GPCRs and ion channels, the FLIPR® High-Throughput Cellular Screening System offers a new high-speed…
Presentations
Functional and mechanistic neurotoxicity profiling using human iPSC-derived neural 3D cultures
Functional and mechanistic neurotoxicity profiling using human iPSC-derived neural 3D cultures
Expertise in human iPSC-derived neural and cardiac platforms; Focus on adapting platforms to HTS and producing assay-ready solutions to accelerate drug discovery; Neural platform:…
eBook
Quantitate Neuron Potential
Quantitate Neuron Potential
Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.
eBook
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
Phenotypic Screening With iPSC-Derived Cardiomyocytes and Neurons
This new eBook discusses early drug safety testing using HTS iPSC-derived cells with imaging. Download it now!
Scientific Poster
Neurotoxicity profiling using 3D human iPSC-derived neural spheroids
Neurotoxicity profiling using 3D human iPSC-derived neural spheroids
Test compound effects and screen for neurotoxic chemicals using 3D neural spheroids.
Publications
Rising growth of the global high‑content screening software and services market
Rising growth of the global high‑content screening software and services market
Developments in informatics solutions and high-content imaging instruments, increased focus on cell-based research, and rising government support of research and development for drug…
Customer Breakthrough
University College London uses the SpectraMax i3x reader and FLIPR Tetra System to develop personalized medicines
University College London uses the SpectraMax i3x reader and FLIPR Tetra System to develop personalized medicines
Professor Andrea Townsend-Nicholson is interested in personalised medicine - helping develop bespoke therapeutics for individuals.
Research in Andrea’s group focuses primarily…
Customer Breakthrough
StemoniX uses three of our solutions to validate their microBrain 3D Assay Ready Plates
StemoniX uses three of our solutions to validate their microBrain 3D Assay Ready Plates
Current models available to neuroscience researchers have limitations that hamper the development of new medicines. Complex models, such as post-mortem brains and animal models, more…
Application Note
Measure Calcium Oscillation in iCell Cardiomyocytes2 on the FLIPR Tetra System
Measure Calcium Oscillation in iCell Cardiomyocytes2 on the FLIPR Tetra System
Shorten growth timelines when screening compound effects on calcium transients in beating cardiomyocytes by using the iCell® Cardiomyocytes2 Kit (Cellular Dynamics International).…
Data Sheet
FLIPR Tetra Instrument LED and Filter Combinations
FLIPR Tetra Instrument LED and Filter Combinations
This document provides the information about FLIPR Tetra Instrument LED and Filter Combinations. Explore this document for more detailed information.
Application Note
Characterization of hERG channel blockers using the FLIPR Potassium Assay Kit on the FLIPR Tetra System
Characterization of hERG channel blockers using the FLIPR Potassium Assay Kit on the FLIPR Tetra System
Drug-induced inhibition of the human ether-à-go-go-related gene (hERG) ion channel has been related to the susceptibility of patients to potentially fatal ventricular tachyarrhythmia,…
Application Note
Assessment of drug effects on cardiomyocyte physiology using human iPSC-derived cardiac spheroids
Assessment of drug effects on cardiomyocyte physiology using human iPSC-derived cardiac spheroids
Cell models are becoming more complex in order to better mimic in vivo microenvironments and provide greater predictivity for compound efficacy and toxicity. There is an increasing…
Application Note
Enhance your calcium screens with FLIPR Calcium 6 Assay Kits
Enhance your calcium screens with FLIPR Calcium 6 Assay Kits
FLIPR Calcium Assay Kits from Molecular Devices employ sensitive calcium indicators and proprietary masking dyes to enable researchers to conduct highly sensitive fluorescent screens…
Application Note
Comparison of Photina luminescent calcium mobilization assays on the FlexStation 3 reader and FLIPR Tetra System
Comparison of Photina luminescent calcium mobilization assays on the FlexStation 3 reader and FLIPR Tetra System
G protein-coupled receptors (GPCRs) represent one of the most important therapeutic targets in drug discovery research. GPCRs are membrane-localized proteins that play an important…
Application Note
Introducing ScreenWorks® Peak Pro™ Software for FLIPR Tetra® Cellular Screening Systems
Introducing ScreenWorks® Peak Pro™ Software for FLIPR Tetra® Cellular Screening Systems
ScreenWorks Peak Pro Software: Visualize and analyze the temporal response curves associated with changes in intracellular calcium ion concentration. The new analysis features simplify…
Application Note
Measuring GPCR Activity Using the Tango GPCR Assay on the FLIPR Tetra Fluorimetric Imaging Plate Reader System
Measuring GPCR Activity Using the Tango GPCR Assay on the FLIPR Tetra Fluorimetric Imaging Plate Reader System
The FLIPR Tetra System has long been used for uHTS and HTS of fast-kinetic GPCR and ion channel assays. The limitations of current microplate readers with respect to format and…
Application Note
Live cell Gi- and Gs -coupled GPCR second messenger signaling on the FLIPR Tetra System
Live cell Gi- and Gs -coupled GPCR second messenger signaling on the FLIPR Tetra System
In this application protocol we demonstrate the use of the modified luminescent firefly luciferase-based Promega GloSensor™ cAMP Assay on the FLIPR Tetra® High Throughput Cellular…
Application Note
Homogeneous solution for GPCR assays with FLIPR Calcium 5 Assay Kit
Homogeneous solution for GPCR assays with FLIPR Calcium 5 Assay Kit
Combining a novel fluorophore and proven masking technology, the FLIPR Calcium 5 Assay Kit delivers reliable pharmacology, a larger signal window, and improved assay performance. With…
Application Note
High throughput cardiotoxicity assays using stem cell-derived cardiomyocytes
High throughput cardiotoxicity assays using stem cell-derived cardiomyocytes
Eliminate cardiotoxic compounds and identify potential cardiac drug candidates earlier in the drug discovery process with the FLIPR Tetra System, together with ScreenWorks Peak Pro…
Application Note
Comparison of intracellular calcium measurements with FLIPR Calcium Assay Kit
Comparison of intracellular calcium measurements with FLIPR Calcium Assay Kit
The FLIPR Tetra System delivers a screening platform to address real-time kinetic cell-based assays in 96-, 384- and 1536-well format. Regardless of the FLIPR Tetra System microplate…
Application Note
Development of a Cav 1.3 channel assay using optogenetic methods on the FLIPR Tetra System
Development of a Cav 1.3 channel assay using optogenetic methods on the FLIPR Tetra System
In this study, we demonstrate the novel utility of optogenetic tools to control membrane potential in a reversible and precise fashion for screening state-dependent calcium channel…
Application Note
Development of a cell-based potassium-chloride transporter assay using the FLIPR Potassium Assay Kit
Development of a cell-based potassium-chloride transporter assay using the FLIPR Potassium Assay Kit
The FLIPR Potassium Assay Kit can be used to measure the functional activity of the hKCC2 cation-chloride cotransporter using a homogeneous, no-wash protocol. The assay kit displays a…
Application Note
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
The assessment of cardiotoxicity is important in the early stages of drug discovery to enable elimination of potentially toxic compounds from further development. This is critical to…
Application Note
Optimization of NaV1.5 channel assay with FLIPR Membrane Potential Assay Kits
Optimization of NaV1.5 channel assay with FLIPR Membrane Potential Assay Kits
FLIPR® Membrane Potential (FMP) Assay Kits provide a rapid and reliable fluorescence-based method to detect changes in membrane potential brought about by compounds that modulate or…
Scientific Poster
Evaluation of Cryopreserved Bacmam Transduced Aequorin Cells in 384-and 1536-Well Suspension Cell Assays Using the Aequorin Option for the FLIPRTETRA® System
Evaluation of Cryopreserved Bacmam Transduced Aequorin Cells in 384-and 1536-Well Suspension Cell Assays Using the Aequorin Option for the FLIPRTETRA® System
Explore PDF to learn about the FLIPR TETRA suspension cell system that provides an effective method to perform Aequorin assays over an extended period of time enabling robust HTS.…
Scientific Poster
Fluorescence-Based Neurotransmitter Transporter Uptake Assay in Primary Neuronal Cultures
Fluorescence-Based Neurotransmitter Transporter Uptake Assay in Primary Neuronal Cultures
Read this PDF to know how fluorescence-based assay kit is useful for the measurement of neurotransmitter transporter uptake activity in cell lines stably expressing the human DAT, NE…
Scientific Poster
Ratiometric Calcium Flux Measurements using Fura‐2 Ca2+ Indicator on the FLIPR® Tetra System
Ratiometric Calcium Flux Measurements using Fura‐2 Ca2+ Indicator on the FLIPR® Tetra System
Read this PDF to explore LED light sources with wavelengths at 340 nm and 380 nm which enable ratiometric Fura‐2 measurements on the FLIPR Tetra system.
Scientific Poster
Calcium Release-Activated Channel (CRAC) Assays on the FLIPR® Tetra System: Evaluation of a Novel Fluorescent Calcium Reagent
Calcium Release-Activated Channel (CRAC) Assays on the FLIPR® Tetra System: Evaluation of a Novel Fluorescent Calcium Reagent
Get detailed information about Calcium release-activated calcium channels by exploring this PDF. Learn how CRAC channels are playing an important role in intracellular Ca2+ homeostas…
Scientific Poster
Cell Based Assays on the FLIPR® Tetra System: Comparison of a Novel FLIPR® Calcium Assay to other Fluorescence based Calcium Flux Assays
Cell Based Assays on the FLIPR® Tetra System: Comparison of a Novel FLIPR® Calcium Assay to other Fluorescence based Calcium Flux Assays
Cell based assays on FLIPR Tetra System to compare a novel FLIPRCcalcium 6 and 6-QF assay to other fluorescence-based calcium flux assays.
Scientific Poster
Automated Functional Cellular Analyses of Human iPS-derived Cardiomyocytes
Automated Functional Cellular Analyses of Human iPS-derived Cardiomyocytes
This PDF explores live cell assays for measuring the impact of pharmacological compounds on rate & magnitude of beating cardiomyocytes using high content imaging & electrophy…
Scientific Poster
Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
Learn about cell assays for measuring the impact of pharmacological compounds on rate & magnitude of beating cardiomyocytes using FLIPR Tetra & ImageXpress Micro Systems.
Scientific Poster
A Novel Homogenous Potassium Ion Channel Assay for High-Throughput Screening
A Novel Homogenous Potassium Ion Channel Assay for High-Throughput Screening
Ion channels are a class of membrane proteins that mediate the movement of charged ions across the cell membrane. Potassium channels constitute the largest and most diverse group of…
Brochure
Pin Tool Liquid Handling for the FLIPR Tetra System
Pin Tool Liquid Handling for the FLIPR Tetra System
Explore this data sheet to learn about Pin tool liquid handling for FLIPR Tetra system which allows direct transfer of compounds that have been suspended in 100% DMSO or ethanol.
Data Sheet
FLIPR Tetra Aequorin Option
FLIPR Tetra Aequorin Option
FLIPR Tetra System provides industry-standard platform for real-time kinetic cellular assays which is user-configurable & easy to run and provides unmatched flexibility.
Data Sheet
Calcium assays for the FLIPR System
Calcium assays for the FLIPR System
The FLIPR® Calcium Assay Kits have been the premier choice for measuring changes in intracellular calcium during drug discovery and research. Built as the most comprehensive calcium…
Publications
A Chemical-Biological Similarity-Based Grouping of Complex Substances as a Prototype Approach for Evaluating Chemical Alternatives
A Chemical-Biological Similarity-Based Grouping of Complex Substances as a Prototype Approach for Evaluating Chemical Alternatives
Comparative assessment of potential human health impacts is a critical step in evaluating both chemical alternatives and existing products on the market. Most alternatives assessment…
Publications
High-Content Assay Multiplexing for Toxicity Screening in Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Hepatocytes
High-Content Assay Multiplexing for Toxicity Screening in Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Hepatocytes
Cell-based high-content screening (HCS) assays have become an increasingly attractive alternative to traditional in vitro and in vivo testing in pharmaceutical drug development and t…
Publications
Assessment of Beating Paramaters in Human Induced Pluripotent Stem Cells Enables Quantitative in Vitro Screening for Cadiotoxicity
Assessment of Beating Paramaters in Human Induced Pluripotent Stem Cells Enables Quantitative in Vitro Screening for Cadiotoxicity
Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes show promise for screening during early drug development. Here, we tested a hypothesis that in vitro assessment of m…
Publications
Multiparameter In Vitro Assessment of Compound Effects on Cardiomyocyte Physiology Using iPSC Cells
Multiparameter In Vitro Assessment of Compound Effects on Cardiomyocyte Physiology Using iPSC Cells
A large percentage of drugs fail in clinical studies due to cardiac toxicity; thus, development of sensitive in vitro assays that can evaluate potential adverse effects on cardiomyoc…


Leverage automated, end-to-end workflows to enable complex organoid assays

Structural organization and functional analysis of compound responses in 3D human iPSC-derived cardiac tri-culture microtissues

Disease modeling in the 21st century: Automated organoid assays with 3D imaging

Implementing 3D Neural Spheroids in Drug Discovery

Toxicity studies of iPSC-derived cardiomyocytes and neuronal spheroids

CiPA initiative and validation of high-throughput methods to characterize compound effects in human iPSC-derived cardiomyocytes on the FLIPR Penta system

(On-demand webinar) FLIPR in drug discovery: Ion channel screening and cardiac safety testing

Optogenetics and the FLIPR: How to Use Light to Set up a Cav1.3 High-Throughput Screening Assay

Latest Advances in Calcium Flux Assays

Innovative Assays for the FLIPR Tetra System

Developing an in Vitro Tl+ Flux Assay for Potassium Channel Agonist Identification

Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery

StemoniX microBrain 3D Assay Ready Plates for HTS
Number of Citations*: 9,480
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
- Dated: Nov 30, 2020Publication Name: Humana Press
Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR)
Ionized calcium plays a major role in the regulation of cellular processes in both eukaryotic and prokaryotic cells (1). A wide variety of cell surface receptors and ion channels utilize a calcium signal to initiate events such as cell motility, contraction, and secretion. To a large extent, the advent of fluorescent indicators of free calcium ion… View moreIonized calcium plays a major role in the regulation of cellular processes in both eukaryotic and prokaryotic cells (1). A wide variety of cell surface receptors and ion channels utilize a calcium signal to initiate events such as cell motility, contraction, and secretion. To a large extent, the advent of fluorescent indicators of free calcium ion concentration that could be loaded into cells in a nondisruptive manner has been responsible for our current knowledge of cellular calcium homeostasis (2). Typically, the fluorescent signal has been monitored using cuvet-based fluorometers or by confocal fluorescent microscopy. Although acceptable for a number of applications, these methods are relatively labor intensive and are not suitable for screening large numbers of compounds.
- Dated: Mar 27, 2017Publication Name: Journal of Biomedical Science
Development and validation of a high-throughput calcium mobilization assay for the orphan receptor GPR88
GPR88 is an orphan G protein-coupled receptor highly expressed in the striatum and is implicated in basal ganglia-associated disorders. However, the receptor functions of GPR88 are still largely unknown due to the lack of potent and selective ligands appropriate for central nervous system investigation. Development of a high-throughput screening… View moreGPR88 is an orphan G protein-coupled receptor highly expressed in the striatum and is implicated in basal ganglia-associated disorders. However, the receptor functions of GPR88 are still largely unknown due to the lack of potent and selective ligands appropriate for central nervous system investigation. Development of a high-throughput screening assay for GPR88 should facilitate the discovery of novel ligands to probe GPR88 functions.
Contributors: Ann M. Decker, Elaine A. Gay, Kelly M. Mathews, Taylor C. Rosa, Tiffany L. Langston, Rangan Maitra & Chunyang Jin
Go to article - Dated: Apr 15, 2005Publication Name: The Journal of Biological Chemistry
c-FLIPR, a New Regulator of Death Receptor-induced Apoptosis
c-FLIPs (c-FLICE inhibitory proteins) play an essential role in regulation of death receptor-induced apoptosis. Multiple splice variants of c-FLIP have been described on the mRNA level; so far only two of them, c-FLIPL and c-FLIPS, had been found to be expressed at the protein level. In this report, we reveal the endogenous expression of a third… View morec-FLIPs (c-FLICE inhibitory proteins) play an essential role in regulation of death receptor-induced apoptosis. Multiple splice variants of c-FLIP have been described on the mRNA level; so far only two of them, c-FLIPL and c-FLIPS, had been found to be expressed at the protein level. In this report, we reveal the endogenous expression of a third isoform of c-FLIP. We demonstrate its presence in a number of T and B cell lines as well as in primary human T cells. We identified this isoform as c-FLIPR, a death effector domain-only splice variant previously identified on the mRNA level. Impor-/tantly, c-FLIPR is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex upon CD95 stimulation. Several properties of c-FLIPR are similar to c-FLIPS: both isoforms have a short half-life, a similar pattern of expression during activation of primary human T cells, and are strongly induced in T cells upon CD3/CD28 costimulation. Taken together, our data demonstrate endogenous expression of c-FLIPR and similar roles of c-FLIPR and c-FLIPS isoforms in death receptor-mediated apoptosis.
Contributors: Alexander Golks, Dirk Brenner, Cornelius Fritsch, Peter H. Krammer‡ and Inna N. Lavrik
Go to article - Dated: Mar 01, 1996Publication Name: Journal of Biomolecular Screening
FLIPR: A New Instrument for Accurate, High Throughput Optical Screening
Modern optical screening assays demand high data throughput along with uncompromised data fidelity. FLIPR (Fluorescent Imaging Plate Reader) was developed to perform quantitative optical screening for cell-based kinetic assays. FLIPR incorporates an integrated design, including low-level optical detection, precise temperature control, and precise… View moreModern optical screening assays demand high data throughput along with uncompromised data fidelity. FLIPR (Fluorescent Imaging Plate Reader) was developed to perform quantitative optical screening for cell-based kinetic assays. FLIPR incorporates an integrated design, including low-level optical detection, precise temperature control, and precise fluid handling, all in one package. The unique aspect of FLIPR is that all 96 wells of a standard microplate are stimulated and optically measured simultaneously. Kinetic updates on all 96 wells can be obtained in under 1 sec, allowing for transient signals to be quantified. Demonstrated applications include measurements of intracellular calcium, intracellular pH, and membrane potential.
FLIPR Penta High-Throughput Cellular Screening System
Product | Part Number |
FLIPR Penta High-Throughput Cellular Screening System | FLIPR PENTA |
Field Installations | |
FLIPR Penta or Tetra Camera Conversion Kit, EMCCD to HSEMCCD | 5069580 |
FLIPR Penta or Tetra Cell Suspension, Field Installation Kit | 0310-5339 |
FLIPR Cycler (Previously TETRACycler) | 5074848 |
Pipettor Heads | |
FLIPR Pipettor Head Kit, 96 | 0200-6071 |
FLIPR Pipettor Head Kit, 384 | 0200-6072 |
FLIPR Pipettor Head Kit, 1536 | 0200-6073 |
FLIPR Pin Tool Head Kit, 384 | 384 Pin Tool HD Kit |
FLIPR Pin Tool Head Kit, 1536 | 1536 Pin Tool HD Kit |
Pipetting Consumables | |
FLIPR Penta or Tetra Pipette tips, black, non-sterile, 96-well 50 racks/case | 9000-0762 |
FLIPR Penta or Tetra Pipette tips, clear, non-sterile, 96-well 50 racks/case | 9000-0761 |
FLIPR Penta or Tetra Pipette tips, black, non-sterile, 384-well 50 racks/case | 9000-0764 |
FLIPR Penta or Tetra Pipette tips, clear, non-sterile, 384-well 50 racks/case | 9000-0763 |
FLIPR 1536 tip gasket, non-sterile, 40/case | 9000-0746 |
FLIPR 1536 tip block | 0200-6112 |
FLIPR Pin Tool head, 384 | 384 Pin Tool |
FLIPR Pin Tool head, 1536 | 1536 Pin Tool |
Optical Accessories | |
LED Modules and Emission filter accessories are available for both versions of the FLIPR Penta or Tetra instrument for Calcium sensitive dyes, Membrane Potential dyes, Voltage Sensor dyes and other applications. Please follow this link to a .pdf of the wavelengths and ordering information | |
Optional Cell Suspension System and Consumables | |
FLIPR Suspension Reservoir | 0200-6222 |
FLIPR 250 mL Spinner Flask Assembly | 0200-6223 |
FLIPR 500 mL Spinner Flask Assembly | 0200-6224 |
FLIPR 1 L Spinner Flask Assembly | 0200-6225 |
FLIPR 2 L Spinner Flask Assembly | 0200-6226 |
FLIPR 3 L Spinner Flask Assembly | 0200-6227 |
FLIPR Potassium Assay Kit | |
Evaluation Kit | R8330 |
Explorer Kit | R8222 |
Bulk Kit | R8223 |
FLIPR Calcium 4 Assay Kit | |
Explorer Kit | R8142 |
Bulk Kit | R8141 |
Express Kit | R8143 |
FLIPR Calcium 5 Assay Kit | |
Explorer Kit | R8185 |
Bulk Kit | R8186 |
Express Kit | R8187 |
FLIPR Calcium 6 Assay Kit | |
Evaluation Kit (1 Plate) | R8194 |
Explorer Kit (10 Plates) | R8190 |
Bulk Kit (100 Plates) | R8191 |
Express Kit (100 Plates) | R8195 |
FLIPR Membrane Potential Assay Kit | |
Evaluation Kit | R8128 |
Explorer Kit RED | R8126 |
Bulk Kit RED | R8123 |
Explorer Kit BLUE | R8042 |
Bulk Kit BLUE | R8034 |
FLIPR Calcium 6-QF Assay Kit | |
Explorer Kit | R8192 |
Bulk Kit | R8193 |
Express Kit | R8196 |
1. Please contact your local Molecular Devices Sales Representative for details regarding this configure to-order item.
FLIPR Cycler and Robotic Integration
Optional Cell Suspension System and Consumables
An optional cell management system for the FLIPR Penta and Tetra Systems simplifies luminescent calcium mobilization assays and increases throughput by eliminating the need to plate cells and the need for separate compound plates during aequorin assays. Cells are held in spinner flask which maintains a uniform cell suspension and promotes assay robustness. During the assay, cells are pumped to the reservoir for pipetting and returned to the spinner flask. Adjustable stir speed, pump speeds and pipetting parameters simplify the optimization of cell handling for different cell lines. A variety of flask sizes are available to scale the volume for assay development through high throughput screening. Requires Part number 03010-5339 field installation of Cell Suspension system.
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The evaluation of calcium flux is a long accepted, tried and true measure of cellular activity.…

Cardiomyocyte screening
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Cardiotoxicity
Cardiotoxicity or cardiac toxicity is a term used to define chemicals that are toxic to the heart,…

GPCRs (G protein-coupled receptors)
GPCRs or G protein-coupled receptors are proteins located on the cell surface that recognize…

Ion Channel
Ion channels are pores in the cellular membrane that allow the passage of ions across the…

Membrane Potential
There are dozens of identified disorders caused by impaired ion channel function. Ion channels are…

Neuronal assays
The FLIPR Penta System is an excellent tool for monitoring calcium flux related to neuronal…

Potassium Assays
The majority of ion channel drug discovery solutions focus on potassium channels since they are the…

Toxicology
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It…
Customer Breakthrough


SUCCESS STORY
University College London uses the SpectraMax i3x reader and FLIPR Tetra System to develop personalized medicines
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…
How can we help advance your next big discovery?
Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges. How can we help you today?
I’d like to…