FLIPR Penta High-Throughput Cellular Screening System
High-throughput kinetic screening for toxicology and lead compound identification
High-throughput kinetic screening for toxicology and lead compound identification
Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per second and quickly cherry pick events such as Early Afterdepolarization-like events (EAD-like events), fibrillation, and irregular beating.
Since its introduction, the system has developed a reputation for reliability and ease of use. The system can be configured based on library size, detection mode, screening format, assay, and target, making the transition from assay development to lead optimization a seamless process.
ScreenWorks Peak Pro 2 software analysis, powered by pCLAMP 11, gives you the unique ability to characterize calcium oscillation peak data in one software program. Compounds can be flagged that cause waveform irregularities that may indicate hazards for arrhythmia, seizures, or other toxicity effects quickly and without having to export data. Distinctly different calcium oscillations in neurons can also be characterized.
The FLIPR Penta system can be matched to user needs starting with a standard EMCCD camera, a more cost-effective solution that is perfect for GPCR and Membrane Potential assays, or with the new HS EMCCD that can capture up to 100 images per second and offers exceptional luminescence performance. The camera, LEDs, and filters can be configured at the time of purchase or later when assay needs change.
Pipette heads can be changed in minutes without the use of tools from 96, 384, or 1536 channels to accommodate assay throughput needs.
ScreenWorks software seamlessly incorporates drag-and-drop protocols, intuitive buttons, and color-coded plate layout to simplify workflows. View signal traces, EC50 curves, and Z factors on-the-fly without having to export data to another software program.
Purchasing a FLIPR system from Molecular Devices assures users that they are investing in a technology that can grow with them. Hardware, software, and assay kits have been validated on the system by an industry-renowned leader with over 40 years of experience.
The drug discovery process has shared methods, yet each project is unique. The Advanced Workflow Engineering Solutions Team offers a variety of customized services from automated compound plate delivery to fully integrated workstations with automated incubation.
The FLIPR Penta system utilizes our ScreenWorks Software to define and run experimental protocols. Using a drag-and-drop interface, protocols can be easily set up to include:
The evaluation of calcium flux is a long accepted, tried and true measure of cellular activity. Calcium flux can be used as a measurement for a host of cellular processes including neurotransmitter release, GPCR activity, voltage or ligand gated ion channels, and cardiomyocyte beat patterns, among many others. The FLIPR Penta System is the drug discovery tool for evaluation of calcium flux in high and ultra-high throughput due to its ease of use, sensitivity and user configurability.
Early prediction of drug-induced functional cardiotoxicity requires robust in vitro systems suitable for high-throughput screening. Readily-available iPSC-derived human cardiomyocytes may be used in conjunction with calcium-sensitive dyes, and beat rates are monitored as changes in intracellular calcium. These calcium peaks can be analyzed with our ScreenWorks PeakPro software which provides the investigator a suite of powerful tools for quantification of cardiomyocyte activity.
Cardiotoxicity or cardiac toxicity is a term used to define chemicals that are toxic to the heart, causing muscle damage or heart electrophysiology dysfunction.
The assessment of cardiotoxicity is important in the early stages of drug discovery to eliminate potentially toxic compounds from further development.
GPCRs or G protein-coupled receptors are proteins located on the cell surface that recognize extracellular substances and transmit signals across the cell membrane. GPCRs do this by activating guanine nucleotide-binding proteins (G protein) that are responsible for signal transduction inside the cell.
Ion channels are pores in the cellular membrane that allow the passage of ions across the impermeant lipid cell membrane. The flow of calcium, potassium, and sodium is very important in many cellular processes such as muscle contraction in the heart, pancreatic insulin release, the transmission of impulses in the nervous system such as pain, and T cell activation.
There are dozens of identified disorders caused by impaired ion channel function. Ion channels are important drug discovery targets because they are highly druggable and specific. The FLIPR® Membrane Potential Assay Kits provide a fast, simple and reliable fluorescence-based assay for detecting changes in voltage across the cell membrane mediated by ion channel to evaluate target or off-target effects based on the application.
The FLIPR Penta System is an excellent tool for monitoring calcium flux related to neuronal activity. Newly developed commercially available iPSC neurons have made high-throughput neuronal screening a reality. These cells and assays are essential for monitoring a variety of factors including neural cell culture conditions, drug compound effects, and the effect of environmental neurotoxins. Neuronal activity and neurotransmitter release can be evaluated with fluorescence-based calcium indicator dyes combining fast, simple, and powerful assays with scalable throughput.
The majority of ion channel drug discovery solutions focus on potassium channels since they are the most prevalent type of ion channel, functioning in cardiac muscle action and hormone release. Potassium assay kits can be used to measure the effect of drug molecules on thallium transport through the potassium channel as a surrogate for potassium channel activity.
Potassium assay resources:
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It is a growing concern in our world today as we are exposed to more and more chemicals, both in our environment and in the products we use.
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Bring simplicity to your multi-user environment with unique protocols utilizing ScreenWorks® Software on the FLIPR® Penta High-Throughput Cellular Screening System. The software cont…
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FLIPR® Penta and Tetra Systems Instrument LED and filter combinations.
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Neurological disorders impact up to a billion people around the world and appear to be on the rise [1]. In fact, those affecting the central and peripheral nervous systems – includin…
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The FLIPR Penta system is powered by a high-speed EMCCD camera and the new ScreenWorks Peak Pro 2 software. The system allows measurement and analysis of complex patterns of calcium…
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Development of biologically relevant, predictive cell-based assays for compound screening and toxicity assessment is a major challenge in drug discovery.
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Development of biologically relevant and predictive cell-based assays for compound screening and toxicity assessment is a major challenge in drug discovery
Brochure
Building on the success of the industry-leading FLIPR® platform for monitoring of GPCRs and ion channels, the FLIPR® High-Throughput Cellular Screening System offers a new high-speed…
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Expertise in human iPSC-derived neural and cardiac platforms; Focus on adapting platforms to HTS and producing assay-ready solutions to accelerate drug discovery; Neural platform:…
eBook
Gain valuable insights into neurobiology, from evaluation of ion channels to analysis of 3D neuron organoids.
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This new eBook discusses early drug safety testing using HTS iPSC-derived cells with imaging. Download it now!
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Test compound effects and screen for neurotoxic chemicals using 3D neural spheroids.
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This document provides the information about FLIPR Tetra Instrument LED and Filter Combinations. Explore this document for more detailed information.
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Drug-induced inhibition of the human ether-à-go-go-related gene (hERG) ion channel has been related to the susceptibility of patients to potentially fatal ventricular tachyarrhythmia,…
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ScreenWorks Peak Pro Software: Visualize and analyze the temporal response curves associated with changes in intracellular calcium ion concentration. The new analysis features simplify…
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FLIPR® Membrane Potential (FMP) Assay Kits provide a rapid and reliable fluorescence-based method to detect changes in membrane potential brought about by compounds that modulate or…
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Explore PDF to learn about the FLIPR TETRA suspension cell system that provides an effective method to perform Aequorin assays over an extended period of time enabling robust HTS.…
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Read this PDF to explore LED light sources with wavelengths at 340 nm and 380 nm which enable ratiometric Fura‐2 measurements on the FLIPR Tetra system.
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Cell based assays on FLIPR Tetra System to compare a novel FLIPRCcalcium 6 and 6-QF assay to other fluorescence-based calcium flux assays.
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Learn about cell assays for measuring the impact of pharmacological compounds on rate & magnitude of beating cardiomyocytes using FLIPR Tetra & ImageXpress Micro Systems.
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FLIPR Penta High-Throughput Cellular Screening System
Leverage automated, end-to-end workflows to enable complex organoid assays
Structural organization and functional analysis of compound responses in 3D human iPSC-derived cardiac tri-culture microtissues
Disease modeling in the 21st century: Automated organoid assays with 3D imaging
Implementing 3D Neural Spheroids in Drug Discovery
Toxicity studies of iPSC-derived cardiomyocytes and neuronal spheroids
CiPA initiative and validation of high-throughput methods to characterize compound effects in human iPSC-derived cardiomyocytes on the FLIPR Penta system
(On-demand webinar) FLIPR in drug discovery: Ion channel screening and cardiac safety testing
Optogenetics and the FLIPR: How to Use Light to Set up a Cav1.3 High-Throughput Screening Assay
Latest Advances in Calcium Flux Assays
Innovative Assays for the FLIPR Tetra System
Developing an in Vitro Tl+ Flux Assay for Potassium Channel Agonist Identification
Novel in Vitro Assay Tools for Cardiac Toxicity and Discovery
StemoniX microBrain 3D Assay Ready Plates for HTS
Latest Citations: For a complete list, please click here .
*Source: https://scholar.google.com/
Ionized calcium plays a major role in the regulation of cellular processes in both eukaryotic and prokaryotic cells (1). A wide variety of cell surface receptors and ion channels utilize a calcium signal to initiate events such as cell motility, contraction, and secretion. To a large extent, the advent of fluorescent indicators of free calcium ion concentration that could be loaded into cells in a nondisruptive manner has been responsible for our current knowledge of cellular calcium homeostasis (2). Typically, the fluorescent signal has been monitored using cuvet-based fluorometers or by confocal fluorescent microscopy. Although acceptable for a number of applications, these methods are relatively labor intensive and are not suitable for screening large numbers of compounds.
GPR88 is an orphan G protein-coupled receptor highly expressed in the striatum and is implicated in basal ganglia-associated disorders. However, the receptor functions of GPR88 are still largely unknown due to the lack of potent and selective ligands appropriate for central nervous system investigation. Development of a high-throughput screening assay for GPR88 should facilitate the discovery of novel ligands to probe GPR88 functions.
c-FLIPs (c-FLICE inhibitory proteins) play an essential role in regulation of death receptor-induced apoptosis. Multiple splice variants of c-FLIP have been described on the mRNA level; so far only two of them, c-FLIPL and c-FLIPS, had been found to be expressed at the protein level. In this report, we reveal the endogenous expression of a third isoform of c-FLIP. We demonstrate its presence in a number of T and B cell lines as well as in primary human T cells. We identified this isoform as c-FLIPR, a death effector domain-only splice variant previously identified on the mRNA level. Impor-/tantly, c-FLIPR is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex upon CD95 stimulation. Several properties of c-FLIPR are similar to c-FLIPS: both isoforms have a short half-life, a similar pattern of expression during activation of primary human T cells, and are strongly induced in T cells upon CD3/CD28 costimulation. Taken together, our data demonstrate endogenous expression of c-FLIPR and similar roles of c-FLIPR and c-FLIPS isoforms in death receptor-mediated apoptosis.
Modern optical screening assays demand high data throughput along with uncompromised data fidelity. FLIPR (Fluorescent Imaging Plate Reader) was developed to perform quantitative optical screening for cell-based kinetic assays. FLIPR incorporates an integrated design, including low-level optical detection, precise temperature control, and precise fluid handling, all in one package. The unique aspect of FLIPR is that all 96 wells of a standard microplate are stimulated and optically measured simultaneously. Kinetic updates on all 96 wells can be obtained in under 1 sec, allowing for transient signals to be quantified. Demonstrated applications include measurements of intracellular calcium, intracellular pH, and membrane potential.
| Product | Part Number |
| FLIPR Penta High-Throughput Cellular Screening System | FLIPR PENTA |
| Field Installations | |
| FLIPR Penta or Tetra Camera Conversion Kit, EMCCD to HSEMCCD | 5069580 |
| FLIPR Penta or Tetra Cell Suspension, Field Installation Kit | 0310-5339 |
| FLIPR Cycler (Previously TETRACycler) | 5074848 |
| Pipettor Heads | |
| FLIPR Pipettor Head Kit, 96 | 0200-6071 |
| FLIPR Pipettor Head Kit, 384 | 0200-6072 |
| FLIPR Pipettor Head Kit, 1536 | 0200-6073 |
| FLIPR Pin Tool Head Kit, 384 | 384 Pin Tool HD Kit |
| FLIPR Pin Tool Head Kit, 1536 | 1536 Pin Tool HD Kit |
| Pipetting Consumables | |
| FLIPR Penta or Tetra Pipette tips, black, non-sterile, 96-well 50 racks/case | 9000-0762 |
| FLIPR Penta or Tetra Pipette tips, clear, non-sterile, 96-well 50 racks/case | 9000-0761 |
| FLIPR Penta or Tetra Pipette tips, black, non-sterile, 384-well 50 racks/case | 9000-0764 |
| FLIPR Penta or Tetra Pipette tips, clear, non-sterile, 384-well 50 racks/case | 9000-0763 |
| FLIPR 1536 tip gasket, non-sterile, 40/case | 9000-0746 |
| FLIPR 1536 tip block | 0200-6112 |
| FLIPR Pin Tool head, 384 | 384 Pin Tool |
| FLIPR Pin Tool head, 1536 | 1536 Pin Tool |
| Optical Accessories | |
| LED Modules and Emission filter accessories are available for both versions of the FLIPR Penta or Tetra instrument for Calcium sensitive dyes, Membrane Potential dyes, Voltage Sensor dyes and other applications. Please follow this link to a .pdf of the wavelengths and ordering information | |
| Optional Cell Suspension System and Consumables | |
| FLIPR Suspension Reservoir | 0200-6222 |
| FLIPR 250 mL Spinner Flask Assembly | 0200-6223 |
| FLIPR 500 mL Spinner Flask Assembly | 0200-6224 |
| FLIPR 1 L Spinner Flask Assembly | 0200-6225 |
| FLIPR 2 L Spinner Flask Assembly | 0200-6226 |
| FLIPR 3 L Spinner Flask Assembly | 0200-6227 |
| FLIPR Potassium Assay Kit | |
| Evaluation Kit | R8330 |
| Explorer Kit | R8222 |
| Bulk Kit | R8223 |
| FLIPR Calcium 4 Assay Kit | |
| Explorer Kit | R8142 |
| Bulk Kit | R8141 |
| Express Kit | R8143 |
| FLIPR Calcium 5 Assay Kit | |
| Explorer Kit | R8185 |
| Bulk Kit | R8186 |
| Express Kit | R8187 |
| FLIPR Calcium 6 Assay Kit | |
| Evaluation Kit (1 Plate) | R8194 |
| Explorer Kit (10 Plates) | R8190 |
| Bulk Kit (100 Plates) | R8191 |
| Express Kit (100 Plates) | R8195 |
| FLIPR Membrane Potential Assay Kit | |
| Evaluation Kit | R8128 |
| Explorer Kit RED | R8126 |
| Bulk Kit RED | R8123 |
| Explorer Kit BLUE | R8042 |
| Bulk Kit BLUE | R8034 |
| FLIPR Calcium 6-QF Assay Kit | |
| Explorer Kit | R8192 |
| Bulk Kit | R8193 |
| Express Kit | R8196 |
1. Please contact your local Molecular Devices Sales Representative for details regarding this configure to-order item.
FLIPR Cycler and Robotic Integration
Optional Cell Suspension System and Consumables
An optional cell management system for the FLIPR Penta and Tetra Systems simplifies luminescent calcium mobilization assays and increases throughput by eliminating the need to plate cells and the need for separate compound plates during aequorin assays. Cells are held in spinner flask which maintains a uniform cell suspension and promotes assay robustness. During the assay, cells are pumped to the reservoir for pipetting and returned to the spinner flask. Adjustable stir speed, pump speeds and pipetting parameters simplify the optimization of cell handling for different cell lines. A variety of flask sizes are available to scale the volume for assay development through high throughput screening. Requires Part number 03010-5339 field installation of Cell Suspension system.
Transform your incredible images into captivating results
The evaluation of calcium flux is a long accepted, tried and true measure of cellular activity.…
Early prediction of drug-induced functional cardiotoxicity requires robust in vitro systems…
Cardiotoxicity or cardiac toxicity is a term used to define chemicals that are toxic to the heart,…
GPCRs or G protein-coupled receptors are proteins located on the cell surface that recognize…
Ion channels are pores in the cellular membrane that allow the passage of ions across the…
There are dozens of identified disorders caused by impaired ion channel function. Ion channels are…
The FLIPR Penta System is an excellent tool for monitoring calcium flux related to neuronal…
The majority of ion channel drug discovery solutions focus on potassium channels since they are the…
Toxicology is the study of adverse effects of natural or man-made chemicals on living organism. It…
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