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      The emerging 3D biology field uses human model systems like organoids to revolutionize drug discovery and our understanding of disease.

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      Molecular Devices adds proprietary patient-derived organoid technology with acquisition of Cellesce

      Dec 06, 2022

      • First-of-its-kind technology from Cellesce creates consistent patient-derived organoids for large-scale drug screening
      • Acquisition strengthens Molecular Devices’ position as a 3D biology solutions innovator
      • Combined expertise will accelerate industry adoption of physiologically-relevant cell models for drug discovery

       

      Visit the OIC

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      Cellsce
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      3D Biology: The paradigm shift in next-generation drug discovery [Interactive infographic]
      Apr 19, 2023The drug discovery landscape is changing. Bringing a drug to market has historically been time-consuming and costly, with many candidates failing in the first phase of…
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      MOLECULAR DEVICES EXPANDS GLOBAL R&D HUB IN AUSTRIA

      Oct 12, 2022        
      Larger site will be future home of the Organoid Innovation Center – Salzburg, a collaborative space for advancing automated cell line development, organoid development, and screening solutions to improve drug discovery

       

      Visit the OIC

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      Silver Sponsor Molecular Devices at Society for Laboratory Automation and Screening 2023 International Conference and Exhibition
      Feb 22, 2023 Showcasing new industry collaborations, automated technology, and workflow innovations that span 3D biology, cell line development, and drug…
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      Conference | North America | Boston, MA USA– Jun 14 – Jun 17, 2023 Booth 305  The world's best and brightest in stem cell research and regenerative medicine convene once a year for the ISSCR Annual Meeting, and you…
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      Conference | Europe | Berlin, Germany, Europe– Jun 26 – Jun 30, 2023 Microphysiological systems (MPS) comprise a number of bioengineering breakthroughs that reproduce organ architecture and function in vitro. Fueled by…
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  4. SLAS 2023 snapshot: innovations, learnings, and what you may have missed
Molecular Devices Lab Notes

SLAS 2023 snapshot: innovations, learnings, and what you may have missed

  • May 3, 2023
  • Laura Dranschak

We caught up with Laura Dranschak, Director of Commercial Operations & Custom Solutions, to catch up on all things SLAS 2023. See what all the buzz was about on the show floor, what the automation industry holds for labs of the future, and download our latest automation research poster on 3D complex assays.

  • What was the vibe like at SLAS 2023?
    • The excitement and interest in robotics and automation were contagious. The industry is putting drug discovery at the center of innovation and customers are driving this journey by embracing automation and its value.
  • Any new products or innovations that stood out?
    • SLAS showcased many innovative products driven by customer feedback and collaboration.
      Our own innovative solutions showcased at SLAS were driven by customers as well. For instance, the QPix XE, a smaller version of our industry leading QPix automated colony picker, is a direct result of customer requests for a bench top colony picker that is affordable for academics and startups. Artificial intelligence (AI) is rapidly evolving—faster, better, and increasingly flexible science backed by data analytics and customer 360 insight, enabling a holistic approach to customer workflows.
      QPix XE
  • What were the popular topics at the event?
    • Conversations were less about assay automation and increasingly about complete end-to-end workflow automation. Automating single-cell printing through monoclonality assurance to organoid cell line creation. Other popular topics included customer accessibility, data analysis trends, and compliance, just to name a few.
  • Tell us about your Automated Workflow Solutions team that was at the show.
    • Automating workflows requires multiple roles working together to execute on customer goals. From sales through procurement, each project is a new challenge. No one associate is an expert in everything, so cross-company collaboration between integrators and vendors is essential for furthering advancements in lab automation. It was great to see the networking between companies at SLAS. Executing excellent custom instrumentation and automated workflows demands multi-faceted and experienced associates. This need is underscored for Molecular Devices, as we also manufacture the core research instrumentation within these workflows. Our team needs not only the expertise of a traditional automation integrator, but also our own instrumentation and literacy in a multitude of automation scheduling software programs. My team leaders Madhavee Buddhikot, Joseph Erthal, and Constantin Radu have a combined 35 years with Molecular Devices in a variety of roles ranging from Technical Support through Service. This question made me curious about the team as a whole, including myself. The team has over 115 years of experience at Molecular Devices ranging from optical engineering to workflow execution. This longevity of tenure gives Molecular Devices Custom & Automated Solutions unmatched agility and flexibility from inquiry to post-installation.                

      Who would you rather design an addition for your home? The team that designed and built the home or an unknown contractor? The original team offers incredible value from adjusting design on the fly to rapid feasibility assessment. Similarly, my team consists of many former R&D associates who now customize and create workflows with the same instrumentation they brought to market.              

      Automated Lab Components
  • How does workflow automation help researchers regain control of their schedules?
    • Science does not follow a M-F schedule, and many weekends and holidays are spent in the lab. Workflow automation helps researchers regain control by increasing efficiency, throughput, and experimental confidence. Not to mention reducing the "wait, what tube?" ruminations.
  • Any parting words?
    • Researchers trust us with their science. Projects are rapidly growing in volume and complexity and, as an industry, we are continuously improving and rising to the challenge. Being part of the solution feels like we’ve come full circle.                

      I had chills leaving SLAS. Helping others is something that motivates and excites me. Many of us go to sleep each night hoping a loved one is cured from diseases like cancer to MS & ALS to lupus. Thanks to the impressive innovation and collaboration highlighted at SLAS, giving someone more time with their loved ones or relief from pain or a cure to cancer are all closer to reality.

Take a look at the automation solutions shared at the show

  • Automating patient-derived colorectal cancer organoids for high-throughput screening
  • Automation of 3D bioprinting assays for high-content imaging and assessment of compound effects
  • Leveraging Automated Workflows to Enable Complex Organoid Assays
  • Novel analysis of neural outgrowth in 3D human brain micro-tissues
  • Optimizing Animal free CloneDetect assay with Protein G or A to maximize the sensitivity of real-time detection of human IgG antibody production for therapeutic protein engineering and cell line development using ClonePix System
  • Single-cell dispensing and screening of cell lines for monoclonality verification using the impedance-based single-cell dispenser and high-throughput fluorescence-based imager

Automating patient-derived colorectal cancer organoids for high-throughput screening

Automating patient-derived colorectal cancer organoids for high-throughput screening

Presented by Angeline Lim, PhD

For this poster, we developed an end-to-end, automated workflow starting with assay-ready colorectal cancer organoids that tracked the effects of various compounds on colorectal organoid size, morphology, texture, and additional morphological and phenotypic readouts. Overall, our results show the superior potential of PDOs vs. other tissues in both precision medicine and high-throughput drug discovery applications when using automation with high-content imaging.

Download poster

Automation of 3D bioprinting assays for high-content imaging and assessment of compound effects

Automation of 3D bioprinting assays for high-content imaging and assessment of compound effects

Presented by Prathyushakrishna Macha, PhD

The automation of a 3D cell model results in a significant reduction in the time and effort involved, as well as an increase in assay precision and throughput. Here we describe methods for an automated generation of organoids and 3D models using automated 3D bioprinting. An increase in throughput and ease of operation was achieved through automation. Also, imaging and data analysis methods provided valuable information about complex compound effects in 3D printed and cell-tissue-engineered models

Download poster

Leveraging Automated Workflows to Enable Complex Organoid Assays

Leveraging Automated Workflows to Enable Complex Organoid Assays

Presented by Oksana Sirenko, PhD

In this poster we demonstrate the tools for increasing throughput and automation in organoid assays and provide strategies aimed at enabling reliable, scalable, and reproducible cell culturing, monitoring, imaging, and analysis of complex biological models.

Download poster

Novel analysis of neural outgrowth in 3D human brain micro-tissues

Novel analysis of neural outgrowth in 3D human brain micro-tissues

Presented by Angeline Lim, PhD

In this study, we reconstructed RealBrain® micro-tissues in 3D and developed a method to quantify the number of neurons and their respective outgrowths. With this approach, we confirmed the efficacy of the compounds with the quantification of neurite outgrowth analysis and found that NGF and PD acid significantly increase, while Rotenone reduces the number of neurites compared to the control. Thus, this type of analysis can be used in multiple applications, such as assessing the effects of compounds on neurons and neural networks.

Download poster

Optimizing Animal free CloneDetect assay with Protein G or A to maximize the sensitivity of real-time detection of human IgG antibody production for therapeutic protein engineering and cell line development using ClonePix System

Optimizing Animal free CloneDetect assay with Protein G or A to maximize the sensitivity of real-time detection of human IgG antibody production for therapeutic protein engineering and cell line development using ClonePix System

Presented by Paula Zadek, PhD

In this study, we explore the usage of recombinant Pro A or Pro G combined with CloneDetect K8495 to enhance the fluorescent signal for IgG detection/quantification. The results have shown that adding Pro G at the final concentration of 1.0 ug/ml produced the best outcome compared to the control assay without adding Pro G optimizer. The combination of Pro G and CloneDetect K8495 amplified the fluorescent signal and improved detection, due to the stabilization of the complexes formed by the target protein, Pro G, and CloneDetect reagent. This approach can be applied to a variety of cell line models that express proteins that bind to the stabilized complex formed with CloneDetect.

Download poster

Single-cell dispensing and screening of cell lines for monoclonality verification using the impedance-based single-cell dispenser and high-throughput fluorescence-based imager

Single-cell dispensing and screening of cell lines for monoclonality verification using the impedance-based single-cell dispenser and high-throughput fluorescence-based imager

Presented by Prathyushakrishna Macha, PhD

In this study, we demonstrate a workflow to increase throughput and automate single-cell isolation procedures and clonal outgrowth compared to limiting dilution method. Combining single-cell dispensing and imaging into a workflow provides an innovative and simple impedance-based method for the isolation of intact single cells and imaging. Its incorporation into an automated platform allows the increase in dispensing throughput and reliability and has the potential to facilitate gene editing. In addition, it can generate multiple engineered cell lines that carry specific indel and can facilitate the development of several bioprocess developments or drug screening studies.

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To summarize, SLAS 2023 was an event full of innovation and collaboration with industry-wide focus squarely on the customers at the center of innovation—and automation is driving this journey. As an industry, we are continuously improving to meet the growing volume and complexity of research projects. Through workflow automation, researchers can increase efficiency, throughput, and experimental confidence to advance discoveries and improve the quality of life everywhere.

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