A real-time clone screening reagent for clonal cell antibody production in cell line development
Key Features
- Real-time in situ detection, no sample preparation or handling required
- Cell-culture validated, sterile and azide-free
- Regulatory friendly, safe to use on cell substrates
- High specificity and affinity for all sub-classes of mouse or human IgGs
- Stability in semi-solid culture media over prolonged periods of culture
- Simple, unit concentrations for optimize performance on ClonePix Systems
- Compatible with other cell analyzer instrument with green fluorescent channels, e.g. CloneSelect® Imager-FL, CloneSelect® Single Cell Printer (SCP), Flow Cytometry (FACs)
New and Improved CloneDetect
Image, analyze, rank and pick secreting clones
CloneDetect is a FITC fluorescein-labeled goat polyclonal antibody with high affinity and specificity for all subclasses of mouse or human IgG. CloneDetect has been manufactured to ISO9001 Quality Assurance guidelines. All raw materials are from USDA registered farm and clinically certified healthy animals. Final products are subjected to safety assessment and pass sterility, viral and mycoplasma test for easy transition to GMP drug development environment.
For applications required to satisfy highest regulatory demands, Recombinant CloneDetect offers a unique recombinant alternative. Recombinant CloneDetect is a FITC fluorescein labeled recombinant monoclonal antibody molecule (mouse IgG), which are produced by engineered CHO cells. The product has a high specificity and affinity for human IgG Fc region. In addition to subjected to a rigorous regimen of safety assessment of sterility, mycoplasma and a panel of adventitious viral agents, each batch comes with certificate of analysis of endotoxin levels.
Screen and select mammalian clones secreting mouse or human IgG
CloneDetect - Human
|
CloneDetect |
Target |
Specificity |
BSA-Free |
Sterile, |
Mycoplasma, |
Endotoxin |
Clonality |
Origin |
|
|---|---|---|---|---|---|---|---|---|---|
| Human IgG (H+L) Specific, Fluorescein |
#K8200 |
Human |
IgG (H+L) |
No |
Yes |
Yes |
No |
Polyclonal |
Sheep |
| Human IgG (H+L) Specific, Fluorescein, BSA Free |
#K8202 |
Human |
IgG (H+L) |
Yes |
Yes |
Yes |
No |
Polyclonal |
Sheep |
| Human IgG (FC) Specific, Fluorescein |
#K8205 |
Human |
IgG Fc |
No |
Yes |
No |
No |
Polyclonal |
Sheep |
CloneDetect - Mouse
|
Clone Detect |
Target |
Specificity |
BSA-Free |
Sterile, |
Mycoplasma, |
Endotoxin |
Clonality |
Origin |
|
|---|---|---|---|---|---|---|---|---|---|
| Mouse IgG (H+L) Specific, Fluorescein |
#K8220 |
Mouse |
IgG (H+L) |
No |
Yes |
No |
No |
Polyclonal |
Sheep |
| Mouse IgG (Fc) Specific, Fluorescein |
#K8225 |
Mouse |
IgG Fc |
No |
Yes |
No |
No |
Polyclonal |
Sheep |
| Updated Mouse IgG (Fc) Specific, Fluorescein |
#K8425 (New) |
Mouse |
IgG Fc |
No |
Yes |
No |
No |
Polyclonal |
Goat |
| Updated Mouse IgG (H+L) Specific, Fluorescein |
#K8420 (New) |
Mouse |
IgG (H+L) |
No |
Yes |
No |
No |
Polyclonal |
Goat |
CloneDetect
|
Clone Detect |
Target |
Specificity |
BSA-Free |
Sterile, |
Mycoplasma, |
Endotoxin |
Clonality |
Origin |
|
|---|---|---|---|---|---|---|---|---|---|
| CloneDetect anti-human IgG (H+L), FITC labeled Sterile, azide-free |
#K8400 |
Human |
IgG (H+L) |
No |
Yes |
Yes |
No |
Polyclonal |
Goat |
Recombinant CloneDetect
|
Clone Detect |
Target |
Specificity |
BSA-Free |
Sterile, |
Mycoplasma, |
Endotoxin |
Clonality |
Origin |
|
|---|---|---|---|---|---|---|---|---|---|
| Recombinant CloneDetect, anti-human IgG (Fc), FITC labeled, Sterile, azide-free |
#K8495 |
Human |
IgG Fc |
Yes |
Yes |
Yes |
Yes |
Recombinant Monoclonal |
CHO cells (animal origin free) |
Latest Resources
Resources of CloneDetect
Scientific Poster
Optimizing animal-free CloneDetect assay to maximize the sensitivity of real-time local detection of human IgG antibody production using ClonePix system
Optimizing animal-free CloneDetect assay to maximize the sensitivity of real-time local detection of human IgG antibody production using ClonePix system
How to screen and select clones of interest with high specificity and high efficiency is known as one of the bottlenecks for therapeutic antibody engineering and cell line development.…
Application Note
To achieve over 5 fold of human IgG protein detection signal enhancement with 1 round of CloneDetect K8495 assay optimization
To achieve over 5 fold of human IgG protein detection signal enhancement with 1 round of CloneDetect K8495 assay optimization
Protein G (Pro G) is a large cell surface binding protein that has a repeating 55-residue domain that binds with high affinity to the FC region of IgG. Together with Protein A (Pro A…
Data Sheet
CloneDetect
CloneDetect
Added to semi-solid media at the moment of plating, Mouse CloneDetect diffuses freely, creating immunoprecipitation complexes with target immunoglobulins as they are secreted from th…
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Data Sheet
Get to Your High-Value Clones Faster with Complete CHO Media Solution
Get to Your High-Value Clones Faster with Complete CHO Media Solution
Molecular Devices offers complete CHO media solution and high-throughput colony picking and screening technology that is used with optimally-formulated media to maximize productivity…
eBook
CloneMedia™ and XP Media™ CHO Growth A Reagents
CloneMedia™ and XP Media™ CHO Growth A Reagents
Explore CloneMedia & XP Media CHO Growth A Reagents, that provides consistent media formulations throughout all stages of cell line development & antibody discovery workflows…
Brochure
Reagents and Supplies
Reagents and Supplies
Molecular Devices offer products and unrivaled solutions that utilize imaging & intelligent image analysis to support basic research, pharmaceutical & biotherapeutic developm…
Infographic
The Evolution of CHO Cells' Role in Cell Line Development
The Evolution of CHO Cells' Role in Cell Line Development
Check out the evolution timeline of CHO cells from CHO-ori to CHO-K1SV. Read about the properties of CHO cells that make them ideal for cell line development.
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Brochure
CloneSelect Imager
CloneSelect Imager
CloneSelect Imager system produces objective, quantitative, and consistent results in less time to overcome the challenges associated with conventional cell confluence techniques.…
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Scientific Poster
Rapid Monoclonality Verification Methods to Boost Cell Line Development
Rapid Monoclonality Verification Methods to Boost Cell Line Development
Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA.
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Application Note
Confident Identification of Monoclonal CHO-S Cells Grown in Semi-Solid Media using the CloneSelect Imager
Confident Identification of Monoclonal CHO-S Cells Grown in Semi-Solid Media using the CloneSelect Imager
Regulatory criteria to obtain approval of biological drugs are constantly evolving. One of the regulatory metrics for biological cell lines, the digital documentation of monoclonality…
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Brochure
Accelerate Cell Line Development
Accelerate Cell Line Development
Explore PDF to learn how you can accelerate your cell line development and get to your high value hits faster.
ClonePix 2
