High-content imaging solution with proprietary spinning disc confocal

The ImageXpress® Micro Confocal system is a high-content solution that can switch between widefield and confocal imaging of fixed and live cells. It can capture high quality images of whole organisms, thick tissues, 2D and 3D models, and cellular or intracellular events. The spinning disc confocal and sCMOS camera enable imaging of fast and rare events like cardiac cell beating and stem cell differentiation. With the MetaXpress software, the system enables many confocal imaging applications from 3D assay development to screening. 

  • Acquire higher quality images

    Acquire higher quality images

    Capture excellent contrast, high resolution images with our proprietary AgileOptix™ spinning disc confocal technology, wide field of view, and bright light source.

  • Customize image acquisition and analysis

    Customize image acquisition and analysis

    Take ultimate control over acquisition and analysis parameters, which enables many applications from 3D structure analysis to target imaging of specific objects within an organism or cell populations. Run more applications with flexible options and high-performance customizations.

  • Analyze more data in less time

    Analyze more data in less time

    MetaXpress® PowerCore™ software accelerates analysis speed in a high-throughput environment. The software distributes image processing jobs to a multi-CPU environment.

ImageXpress Micro Confocal Video

ImageXpress Micro Confocal Video

Features

  • Wide dynamic range

    Quantify low and high intensity signals in a single image with >3 log dynamic range intensity detection.

  • Exclusive AgileOptix spinning disc confocal

    The technology provides increased sensitivity with custom optics, high-powered, solid state light engines, and sCMOS technology. Swappable disc geometries provide flexibility between speed and resolution.

  • Wide field of view

    Wide field of view enables whole-well confocal imaging and eliminates missed targets.

  • Optional on-board robotic fluidics

    For assays that involve compound addition, well washing, and media exchange, optional on-board robotic fluidics are available.

  • Accurate 3D measurements

    MetaXpress 3D analysis module is optimized for confocal imaging, enabling 3D measurements of volume and distance.

  • Multiple imaging modes

    The system offers phase contrast and brightfield label-free imaging, fluorescence, widefield, colorimetric, and confocal imaging.

*NEW* High-performance customizations for high-content screening solution

Get optimized imaging capabilities including water immersion objectives, high intensity lasers, and deep tissue penetrating, confocal disk module for live cell imaging, hypoxia studies, thick cell samples, and multiplexed assays.

AWES Illustration

Cellular Image Gallery

Micro Confocal High-Content Imaging System
ImageXpress High-content imaging solution
ImageXpress Micro Confocal Imaging System
ImageXpress Micro Imaging System
Micro Confocal Imaging System
 
Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.

Applications of ImageXpress Micro Confocal High-Content Imaging System

  • 3D Cell Imaging and Analysis

    3D Cell Imaging and Analysis

    Three-dimensional (3D) cell models are physiologically relevant and more closely represent tissue microenvironments, cell-to-cell interactions, and biological processes that occur in vivo. Now you can generate more predictive data by incorporating technologies like the ImageXpress system with the integrated 3D Analysis Module in MetaXpress® software. This single interface will enable you to meet 3D acquisition and analysis challenges without compromise to throughput or data quality, giving you confidence in your discoveries.

    Read Application Note 

    3D Gel Matrix Assays

    3D Gel Matrix Assays

    The development and integration of three-dimensional (3D) assay models are becoming popular to drive translational biology. Our ImageXpress system allows superior visualization of thick samples by minimizing background fluorescence and increasing sharpness, resulting in accurate image segmentation. Increase the biological relevance of your screening assays with a seamless integration between acquisition and analysis of cells in a 3D space to yield volume, intensity, and distance measurements.

    Read Application Note 

  • Neurite Outgrowth / Neurite Tracing

    Neurite Outgrowth

    Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.

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    Organ-on-a-Chip

    Organ on a Chip Assays

    Emulating organ physiology by co-culturing cells in a supportive 3D matrix and using microfluidic channels to perfuse nutrients or compounds over the resulting cellular structures, is rapidly gaining popularity as a biologically relevant screening model for new drugs or toxicity. The ImageXpress system provides a turnkey solution for both 2D or 3D applications and can accommodate many different types of slide and plate formats.

  • Spheroid Assays

    Spheroid Assays

    Three-dimensional (3D) cells cultured into spheroids provide a more complete and physiologically predictive model especially for cancer research. Acquiring measurements from the larger structure involves acquiring images from different depths within the body of the spheroid and analyzing them in 3D or collapsing into a single 2D stack before analysis. The ImageXpress Micro system and MetaXpress software are designed to make acquisition and analysis of 3D spheroids fast and accurate.

    Read Application Note 

    Toxicity Screening

    Toxicity Screening

    Screening for off-target or toxic effects is very important during the development of new drugs and for the extension of the therapeutic potential of existing molecules. ImageXpress systems are fully integrated hardware and software platforms for automated acquisition and analysis of images for high-throughput cell-based cytotoxicity testing. Configured with optional environmental control, living cell responses or kinetic reactions can be monitored in real time for several days.

    Read Application Note 

Specifications & Options of ImageXpress Micro Confocal High-Content Imaging System

Resources of ImageXpress Micro Confocal High-Content Imaging System

Videos and Demos

3D in Vitro Models

Optimizing high-content screening tools for physiologically-relevant 3D in vitro models

Morphological Characterization of 3D Neuronal Networks in a Microfluidic Platform

Morphological Characterization of 3D Neuronal Networks in a Microfluidic Platform

3D Imaging of Cancer Spheroids

3D Imaging of Cancer Spheroids

High-Content Screening for Identifying miRNAs

High-Content Screening for Identifying miRNAs

Identification of Selective Inhibitors of the STAT3 Signaling Pathway

Identification of Selective Inhibitors of the STAT3 Signaling Pathway

Oliver Kepp and Jayne Hesley - Detect Cell Death Signatures with High Content Imaging

Oliver Kepp and Jayne Hesley - Hallmarks of Cancer - Detect and Quantify Cell Death Signatures with High Content Imaging

Multidimensional ImageXpress Micro Confocal System

Multidimensional High-Throughput Imaging with the New ImageXpress Micro Confocal System

Pushing the Boundaries of High Content Screening

Pushing the Boundaries of High Content Screening

Preparing Assays for Genome-wide RNAi Screens

Preparing Assays for Genome-wide RNAi Screens Using High Content Microscopy

Multiplexed High Content Hepatoxicity Assays

Multiplexed High Content Hepatoxicity Assays Using iPSC-Derived Hepatocytes

High Content Imaging Analysis of Cell Sheet Morphogenesis Utilizing in Virto Tissue Models

High Content Imaging Analysis of Cell Sheet Morphogenesis Utilizing in Virto Tissue Models

Simple and Flexible High Content Imaging of Complex Biological Events

Simple and Flexible High Content Imaging Enabling Quantitation of Complex Biological Events

Contemporary Automation and High Content Imaging Tools

Contemporary Automation and High Content Imaging Tools for Screening Stem Cell-Derived Cardiomyocytes

3D Image Analysis from Subcellular Structures to Spheroids

Implement High-Throughput 3D Image Analysis for Samples from Subcellular Structures to Spheroids

Live Cell Imaging to Investigate Cell Division Timing

Live Cell Imaging to Investigate the Regulation of Cell Division Timing

High-throughput RNA Screening to Identify Host Factors

Using High-throughput RNA Screening to Identify Host Factors That Impact Viral Infection

Application of HCA Tools for Antibody Drug Discovery

Application of HCA Tools for Antibody Drug Discovery

3D Spheroid Assays Using High-Content Imaging

Setting up 3D Spheroid Assays Using High-Content Imaging

Physiologically-relevant tissue models using a high-throughput Organ-on-a-Chip platform

Physiologically-Relevant Tissue Models Using a High-Throughput Organ-on-a-Chip Platform

Pluripotent Stem Cell Applications in Drug Discovery

Emerging Induced Pluripotent Stem Cell Applications in Drug Discovery

StemoniX microBrain 3D Assay Ready Plates for HTS

StemoniX microBrain 3D Assay Ready Plates for HTS

Related Products & Services of ImageXpress Micro Confocal High-Content Imaging System

Scalable, high-performance, high-content screening solutions

Molecular Devices can successfully tailor the ImageXpress Micro Confocal High-Content Imaging System to include customized software and hardware including the features described herein, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated workcell. With over 30 years of experience in the life science industry, you can count on us to deliver quality products and provide worldwide support.

Capture more data at greater
depths for 3D and thick
tissue samples

Download Flyer
3D Tissue Sample3D Tissue

Water Immersion Objectives

Water immersion objectives can offer signal increases of up to 4X† which can help scientists see deeper into 3D and thick tissue samples at lower exposure times.

High-Intensity Lasers

High output laser excitation can reduce exposure times by up to 75%.† The 5-channel laser light source has an output of 400—1,000mw/channel. The 8-channel laser light source (including nIR) with 1,000mw/channel is ideal for customers with increased multiplexing requirements.

  • Obtain sharper images with higher signal-to-noise
  • Generate up to a 2X† boost in scan speed attributed to significantly reduced exposure times
  • Run FRET experiments using lasers for CFP and YFP
Standard
Standard Intensity Image
Laser
High-Intensity Lasers Image

Images taken at the same exposure.

Deep tissue penetrating, confocal disk module

An upgrade to the deep tissue penetrating, confocal disk module, combined with a laser light source, can improve resolution when imaging thick tissue samples†.

  • Improve suppression of out-of-focus light
  • Reduce haze (pinhole crosstalk)
  • Penetrate deeper into thick tissue samples for sharper images
Standard spinning disk
Standard Spinning Disc
Deep tissue penetrating, confocal disk module
Deep Tissue Penetrating with Confocal Disk

Images taken at the same exposure.

Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.

Featured Applications

Customer Breakthrough

Hapix hcs phrama
Hapix hcs phrama

SUCCESS STORY

HCS Pharma uses ImageXpress Micro Confocal Systems for phenotypic screening of physiologically relevant 3D cell models

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Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Maximize flexibility in application workflow and experimental design

Contact our specialist today to discuss how to implement a solution that works for you

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

Maximize flexibility in application workflow and experimental design

Contact our specialist today to discuss how to implement a solution that works for you