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Water Immersion Objectives

What is water immersion technology?

 

What is water immersion technology

Water immersion, in terms of high-content imaging, is the technology that automatically and consistently places a layer of water between the objective (magnification) and the sample.

Many cell imaging systems are designed to have air between the objective and the sample. When using air objectives for 3D imaging, a significant portion of the fluorescent light that is emitted from a sample is refracted away from the objective and becomes lost. This reduces the signal that is collected from the sample, and causes the sample to appear distorted.

Oil objectives have been the gold standard for 3D confocal imaging, because they collect more light from the sample, which increases the signal intensity and enhances the image resolution. However, oil objectives are still problematic, as oil can be difficult to handle in an automated fashion and these objectives can also cause some distortion when imaging large 3D samples.

 

Water immersion objectives for the ImageXpress Micro Confocal system

 

Scientists often experience difficulties screening complex models and obtaining better data quality. With water immersion objectives on the ImageXpress® Micro Confocal High-Content Imaging System, they can increase signal up to four times, improve z-resolution, and decrease optical aberrations for sharper, crisper images.

To address 3D image analysis workflow challenges, our MetaXpress® 3D analysis software module enables scientists to generate greater phenotypic data within a single interface without compromising throughput or data quality, giving scientists confidence in their discoveries.

 

3D assay using water immersion objective

Increased average intensity in 3D assay using water immersion objective. Nuclei of spheroids at 50 ms exposure, maximum projection using confocal imaging.

Water immersion cell count

Data and images were acquired during development using customer samples. Results may vary.

 

 

Gain the sensitivity to capture more phenotypic data at greater depths

Water immersion objectives can offer signal increases of up to 4X which can help scientists see deeper into 3D and thick tissue samples at lower exposure times.

Data and images were acquired during development using customer samples. Results may vary.

 

 

 

 

More great resources

 

  • Gain deeper insights into cellular 3D structures

    Gain deeper insights into cellular 3D structures

    Gain deeper insights into cellular 3D structures with water immersion objectives for high-content imaging. With the increasing interest in using three-dimensional (3D) cultures for assay development and phenotypic screening for a range of cellular models, water immersion objectives are essential for capturing more data at greater depths in 3D structures, such as spheroids and thick tissues.

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    Water immersion objectives for automated high-content imaging

    Water immersion objectives for automated high-content imaging

    Water immersion objectives for automated high-content imaging to improve precision and quality of complex biological assays determining if water immersion objectives, used to improve image quality in complex biological assays, could be used in a high-throughput environment.

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  • 3D image analysis and characterization of angiogenesis

    3D image analysis and characterization of angiogenesis

    3D image analysis and characterization of angiogenesis in organ-on-a-chip model

    • Visualize angiogenic sprouting and 3D reconstitution of structures
    • Perform quantitative assessment of angiogenesis, including number of sprouts, total volume, and average intensity
    • Generate physiologically-relevant results using the OrganoPlate® platform

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    Monitor mitochondria dynamics and phenotype with HCI

    Monitor mitochondria dynamics and phenotype with HCI

    Monitor mitochondria dynamics and phenotype with high-content imaging

    • Achieve better visualization of changes in mitochondrial shape and structure
    • Quantify and measure phenotypic changes in a more efficient and precise manner
    • Understand mechanisms of disease and assess compound toxicity in a variety of cell models

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  • Water immersion technology and high-content imaging: A closer look

    Water immersion technology and high-content imaging: A closer look

    Three-dimensional (3D) cellular assays have emerged as a valuable tool in drug discovery and biological research, as they closely mimic in vivo environments and are shown to generate more physiologically relevant data than many 2D assays. With the increased utilization of 3D cultures for phenotypic screening and toxicity assessment, there is a growing need to optimize assay sensitivity, resolution, and throughput.

    Read more  

    Improve 3D acquisition and analysis with water immersion objectives

    Improve 3D acquisition and analysis with water immersion objectives

    Sebastian Peck is a senior product manager for cellular imaging at Molecular Devices, one of the world's leading providers of high-performance bioanalytical measurement systems, software, and consumables for life science research, pharmaceutical, and biotherapeutic development.

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Resources for Water Immersion Objectives

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Water Immersion Objectives & High-Content Imaging

Gain deeper insights into cellular 3D structures with water immersion objectives