User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
User error: "attributes" is an invalid render array key in Drupal\Core\Render\Element::children() (line 97 of core/lib/Drupal/Core/Render/Element.php).
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Water Immersion Objectives

What is water immersion technology?

What is water immersion technology

Water immersion, in terms of high-content imaging, is the technology that automatically and consistently places a layer of water between the objective (magnification) and the sample.

Many cell imaging systems are designed to have air between the objective and the sample. When using air objectives for 3D imaging, a significant portion of the fluorescent light that is emitted from a sample is refracted away from the objective and becomes lost. This reduces the signal that is collected from the sample, and causes the sample to appear distorted.

Oil objectives have been the gold standard for 3D confocal imaging, because they collect more light from the sample, which increases the signal intensity and enhances the image resolution. However, oil objectives are still problematic, as oil can be difficult to handle in an automated fashion and these objectives can also cause some distortion when imaging large 3D samples.

Water immersion objectives for the ImageXpress Micro Confocal system

Scientists often experience difficulties screening complex models and obtaining better data quality. With water immersion objectives on the ImageXpress® Micro Confocal High-Content Imaging System, they can increase signal up to four times, improve z-resolution, and decrease optical aberrations for sharper, crisper images.

To address 3D image analysis workflow challenges, our MetaXpress® 3D analysis software module enables scientists to generate greater phenotypic data within a single interface without compromising throughput or data quality, giving scientists confidence in their discoveries.

3D assay using water immersion objective

Increased average intensity in 3D assay using water immersion objective. Nuclei of spheroids at 50 ms exposure, maximum projection using confocal imaging.

Water immersion cell count

^†Data and images were acquired during development using customer samples. Results may vary.

Gain the sensitivity to capture more phenotypic data at greater depths

Water immersion objectives can offer signal increases of up to 4X^† which can help scientists see deeper into 3D and thick tissue samples at lower exposure times.

Gain up to 4 times more signal at depth

Improve z-resolution and decrease optical aberrations for more accurate reconstruction of 3D samples.

Gain sharper, crisper images

Better light collection results in images with brighter intensity at lower exposure times, contributing to better quality of data.

Run your samples with confidence

MetaXpress software includes integrated, user-intuitive sensors and alerts to indicate the system health of the water controller system to give you peace of mind when running your samples.

Acquire 3D samples at high throughput

The ImageXpress Micro Confocal system with water immersion objectives automatically replenishes the water during acquisition, so the entire microplate sample can be acquired at high throughput.

Simplify viewing and quantification of 3D structures

Analyze spheroids, microtissues, cells in a 3D matrix, and small organisms that are acquired as a stack of z-planes. Utilize the MetaXpress 3D Analysis Module to evaluate volume, XYZ location, distance to neighboring objects, diameter, depth, various intensity measurements, texture, or number of objects.

Shorten image acquisition time

QuickID targeted acquisition images at low magnification to find objects of interest or rare events, then, automatically acquires high-magnification images with the required wavelengths, z-stack, or time points. This analysis process shortens acquisition time and reduces imaging storage requirements by acquiring selected targets at high-magnification.

^†Data and images were acquired during development using customer samples. Results may vary.

More great resources

Gain deeper insights into cellular 3D structures
Water immersion objectives for automated high-content imaging
3D image analysis and characterization of angiogenesis
Monitor mitochondria dynamics and phenotype with HCI
Water immersion technology and high-content imaging: A closer look
Improve 3D acquisition and analysis with water immersion objectives

Gain deeper insights into cellular 3D structures

Gain deeper insights into cellular 3D structures with water immersion objectives for high-content imaging. With the increasing interest in using three-dimensional (3D) cultures for assay development and phenotypic screening for a range of cellular models, water immersion objectives are essential for capturing more data at greater depths in 3D structures, such as spheroids and thick tissues.

Water immersion objectives for automated high-content imaging to improve precision and quality of complex biological assays determining if water immersion objectives, used to improve image quality in complex biological assays, could be used in a high-throughput environment.

3D image analysis and characterization of angiogenesis in organ-on-a-chip model

Visualize angiogenic sprouting and 3D reconstitution of structures
Perform quantitative assessment of angiogenesis, including number of sprouts, total volume, and average intensity
Generate physiologically-relevant results using the OrganoPlate^® platform

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Monitor mitochondria dynamics and phenotype with HCI

Monitor mitochondria dynamics and phenotype with high-content imaging

Achieve better visualization of changes in mitochondrial shape and structure
Quantify and measure phenotypic changes in a more efficient and precise manner
Understand mechanisms of disease and assess compound toxicity in a variety of cell models

Read application note

Water immersion technology and high-content imaging: A closer look

Three-dimensional (3D) cellular assays have emerged as a valuable tool in drug discovery and biological research, as they closely mimic in vivo environments and are shown to generate more physiologically relevant data than many 2D assays. With the increased utilization of 3D cultures for phenotypic screening and toxicity assessment, there is a growing need to optimize assay sensitivity, resolution, and throughput.

Improve 3D acquisition and analysis with water immersion objectives

Sebastian Peck is a senior product manager for cellular imaging at Molecular Devices, one of the world's leading providers of high-performance bioanalytical measurement systems, software, and consumables for life science research, pharmaceutical, and biotherapeutic development.

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High-performance Customizations

High-performance Customizations

Capture more data at greater depths for 3D and thick tissue samples Scalable, high-performance, high-content screening solutions with the ImageXpress® Micro Confocal system

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Overcome the challenges of high-throughput 3D imaging

Overcome the challenges of high-throughput 3D imaging

Thanks to recent advances in imaging technologies, we are now able to observe and analyze complex cellular networks in three dimensions. Through 3D imaging, we can acquire and analyz…

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7 tips for optimizing your 3D cell imaging and analysis workflow

7 tips for optimizing your 3D cell imaging and analysis workflow

As the field of cell biology moves toward increasing complexity of assays, 3D cell systems have proven to provide more physiologically relevant information as compared to traditional…

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Advancing scientific discovery to improve quality of life

Advancing scientific discovery to improve quality of life

Accomplishing scientific breakthroughs can be a slow and arduous process. However, time is of the essence when dementia, cancer, cardiac disease, COVID-19, and more continue to upend…

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Water immersion technology and high-content imaging: A closer look

Water immersion technology and high-content imaging: A closer look

Three-dimensional (3D) cellular assays have emerged as a valuable tool in drug discovery and biological research, as they closely mimic in vivo environments and are shown to generate…

Download Blog

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Application Note

3D image analysis and characterization of angiogenesis in organ-on-a-chip model

3D image analysis and characterization of angiogenesis in organ-on-a-chip model

Angiogenesis is the physiological process of formation and remodeling of new blood vessels and capillaries from pre-existing blood vessels. This can be achieved through endothelial…

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Monitor mitochondria dynamics and phenotype with high-content imaging

Monitor mitochondria dynamics and phenotype with high-content imaging

Mitochondria are the main energy source for cells and play a key role in regulating cellular metabolism. Mitochondria can change their structures depending on environmental conditions…

Read Application Note

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Scientific Poster

Water immersion objectives for automated high-content imaging to improve precision and quality of complex biological assays

Water immersion objectives for automated high-content imaging to improve precision and quality of complex biological assays

The purpose of these studies was to determine if water immersion objectives, used to improve image quality in complex biological assays, could be used in a high-throughput environment.

Download Scientific Poster

Download Scientific Poster
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Water Immersion Objectives for the ImageXpress Micro Confocal system

Water Immersion Objectives for the ImageXpress Micro Confocal system

Gain the sensitivity to capture more phenotypic data at greater depths. Our high-content imagers and expert team are here to help your lab simplify imaging and analysis of complex, 3D a…

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Download Flyer

Videos & Webinars

Water Immersion Objectives & High-Content Imaging

Gain deeper insights into cellular 3D structures with water immersion objectives

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