Solid state lasers for high-performance laser excitation
With the increasing use of highly-complex cell-based 2D and 3D assays in biologic research, there is a pressing demand for improvements to the capabilities of automated high-content imaging. The ImageXpress® Confocal HT.ai High-Content Imaging System is a high-throughput solution that utilizes an 8-channel high-intensity laser light source to penetrate into deep tissue while reducing exposure times and increasing focus.
The illumination power of the laser light source significantly increases image intensity and assay sensitivity, which is especially important for dim samples. Imaging systems with lasers substantially decrease exposure times, which result in increased imaging speed and assay throughput. 3D imaging especially benefit from the laser light source with higher image intensities and improve image quality, which result in increased assay sensitivity and imaging speed.
**Data and images were acquired during development using customer samples. Results may vary. Highlighted features’ price, time to deliver, and specifications will vary based on mutually agreed technical requirements. Solution requirements may cause adjustment to standard performance.
AgileOptix technolgy with powerful, solid state light engine
AgileOptix technology makes it possible for the system to deliver the sensitivity and throughput needed for demanding applications by combining an advanced solid-state light engine with seven laser lines and eight filter combinations, proprietary spinning disk technology, state-of-the-art optics, and a scientific CMOS sensor.
Molecular Devices can successfully tailor the ImageXpress Confocal system to include customized software and hardware including the features described herein, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated work cell. Check out our new Organoid Innovation Center where we showcase these cutting-edge technologies with novel 3D biology methods to address key challenges of scaling complex 3D biology.
Sales are subject to our Custom Product Purchase Terms available at www.moleculardevices.com/custom-products-purchase-terms.
Laser and LED light source specifications
The new modification of the ImageXpress Confocal HT.ai system equipped with multiline laser light source with matched filters spanning 405 nm to 730 nm excitation wavelength provides drastically increased illumination power to the sample. Laser light source results in brighter images, increased sensitivity, and increased speed and throughput speed of assays. The impact is especially important for the assays where sensitivity and timing are limiting factors.
Decreased exposure times with laser illumination
Confocal imaging and 3D image analysis are especially useful for capturing the complexity of 3D biological assays like organoids and spheroids. Here, we compared a standard imaging system with LED excitation to one with laser excitation.
Imaging 3D cancer spheroids

A. DRAQ5 and Phalloidin-AF488 overlay of an untreated spheroid plus nuclear segmentation and area masks of the 2D projection image. Low magnification QC of spheroid staining could be used to locate treated and control spheroids in the well for accurate centering before acquiring at a higher magnification. B. Exposure times optimized to acquire images of equivalent brightness. Different exposures were required for different staining protocols. C. Speed of acquiring a 96-well spheroid plate in the ImageXpress Confocal HT.ai system at 20X using LED vs laser light sources.

Imaging 3D lung organoids

A. Confocal image of organoid culture (maximum projection), 10X. B. Images were taken using same exposure times for LED or laser light sources, then cell count and average intensities of objects (cells) shown for representative images. C. Exposure times were matched for 14-bit intensity range for both laser and LED. Optimized exposure times and duration of the experiment were substantially reduced by using lasers.

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Organoid Innovation Center
Our lab automation solutions include scientists and engineers who can customize our instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. From incubators, liquid handlers, and robotics to customized software and hardware—and with over 35 years of experience in the life science industry—you can count on us to deliver quality products and provide worldwide support.
Learn more about how robotics-driven automation workcells and AI-based image analysis can help you develop an efficient, end-to-end workflow for your organoid development process.
3D Cell Models
3D cell cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion characteristics. Utilization of 3D cellular assays adds value to research and screening campaigns, spanning the translational gap between 2D cell cultures and whole-animal models. By reproducing important parameters of the in vivo environment, 3D models can provide unique insight into the behavior of stem cells and developing tissues in vitro.
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Improve sensitivity, speed, and assay quality
Improve sensitivity, speed, and assay quality for complex biological assays
Using a high-power laser light source, the system significantly increases light throughput to the sample, which results in brighter images, increased sensitivity, and increased assay throughput. The impact is especially important for the assays where sensitivity and imaging time are the limiting factors.
Monitor lung organoids using 3D high content imaging
Organoid models have increasingly gained popularity in biologic research and screening to recapitulate complexity of real tissues. To model the in vivo human lungs, we have cultured primary human lung epithelial cells under conditions that promote the formation of 3D structures recapitulating the morphological and functional characteristics of the airway.
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Organoids
Organoids are three-dimensional (3D) multi-cellular microtissues that are designed to closely mimic the complex structure and functionality of human organs. Organoids typically consist of a co-culture of cells which demonstrate a high order of self-assembly to allow for an even better representation of complex in vivo cell responses and interactions, as compared to traditional 2D cell cultures.
Using a laser light source for high-content automated imaging
In this study, we demonstrate improvement in assay sensitivity, precision, and speed of acquisition with a new configuration of the ImageXpress® Confocal HT.ai High-Content Imaging System that utilizes laser light source. The system drastically increases the power of illumination that results in brighter images, increased sensitivity, and increased speed and throughput speed of assays.
Latest Resources
Resources for Confocal Laser Illumination
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Custom Organoid Expansion Service
Custom Organoid Expansion Service
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Brochure
ImageXpress Confocal HT.ai
ImageXpress Confocal HT.ai
The ImageXpress® Confocal HT.ai High-Content Imaging System utilizes a seven-channel laser light source with eight imaging channels to enable highly multi-plexed assays while maintainin…
Publications
Using 3D cancer cell models to push forward personalized medicine
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Scientific Poster
Using a laser light source for high-content automated imaging
Using a laser light source for high-content automated imaging
In this study, we demonstrate improvement in assay sensitivity, precision, and speed of acquisition with a new configuration of the ImageXpress® Confocal HT.ai High-Content Imaging Syst…
Application Note
Improve sensitivity, speed, and assay quality for complex biological assays
Improve sensitivity, speed, and assay quality for complex biological assays
Here, we demonstrate improvement in assay sensitivity, quality, and speed of acquisition with the ImageXpress® Confocal HT.ai, our high-content imaging laser-based system.
Blog
Water immersion technology and high-content imaging: A closer look
Water immersion technology and high-content imaging: A closer look
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Advanced Cell Screening and Imaging Reveals How the Cardiac System Responds in Drug Development
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Improving acquisition and analysis of 3D cell model assays with water immersion objectives
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High-performance Customizations
High-performance Customizations
Capture more data at greater depths for 3D and thick tissue samples Scalable, high-performance, high-content screening solutions with the ImageXpress® Micro Confocal system
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Enhancing 3D Disease Models: Automated, High-Throughput, Phenotypic Screening with Organ-on-a-Chip