APPLICATION NOTE
Screening of 3D spheroids using a robust, sensitive, next-generation high-content imager
Advancing 3D Imaging for Oncology Research: High-Content, High-Throughput Analysis with AI-Driven Precision
In this application note, we demonstrate the performance of the ImageXpress® HCS.ai System for high-throughput imaging and analysis of 3D spheroid models. We show accurate quantification of apoptosis and morphology changes with over 2× improvement in image quality and rapid, reliable autofocus across U-bottom plates. Using IN Carta® Software with both traditional and AI-based segmentation methods—including deep learning via the SINAP module—enable robust analysis even in low-signal conditions, supporting complex drug screening workflows. Together, these capabilities position the ImageXpress HCS.ai System as a powerful, end-to-end solution for advanced 3D imaging and analysis in drug discovery.
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Product family
Imaging
Product primary application
IN Carta Image Analysis Software; ImageXpress HCS.ai High-Content Screening System; MetaXpress High-Content Image Acquisition and Analysis Software; 3D Cell Models; Cancer Research Solutions; Cell Imaging & Analysis; Colorectal Cancer Organoids; Disease Modeling; Organoid Innovation Center; Organoids; Spheroids
CMP
701Rn00000grG5CIAU
Title
Screening of 3D spheroids using a robust, sensitive, next-generation high-content imager
Resource URL
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